Shiga toxigenic, enteroinvasive and enteropathogenic Escherichia coli in fish from experimental fish farm (Layo), Cte dIvoire

Abstract

The objective of this study was to know the sanitary quality of fish coming from Layo farm. Twenty fishes (Oreochromis niloticus), were selected in four ponds and Escherichia coli were isolated in gills and viscera according to microbiological methods. One hundred and twenty strains of E. coli were isolated, and their virulence was performed by polymerase chain reaction (PCR) using specific primers. Eleven (11) strains (9.16%) including 7 strains of gills (11.66%) and 4 strains of viscera (6.66%) had virulence genes eae, Stx1, Stx2 or ial. Atypical Enteropathogenic E. coli (EPEC, eae+, lack of bfp) was isolated from gills (5%) and viscera (1.66%). Shiga toxigenic E. coli (STEC) with genes eae + Stx2, Stx1 and Stx2 were isolated in viscera (5%) and gills (3.33%). For Enteroinvasive E. coli (EIEC), ial gene was isolated in gills (3.33%) but no ipah gene. Enterotoxinogen E. coli (ETEC) with lt gene and Enteroaggregating E. coli (EAEC) with aggA gene were not detected in this study. This study revealed that some fish from Layo farm are carriers of virulent E. coli that can cause serious human diseases and can lead to consumer death if cooking is insufficient or by cross-contamination. This therefore poses a real public health problem.

Molecular characterization and antimicrobial resistance among diarrheagenic and non-diarrheagenic Escherichia coli from healthy and diseased freshwater farmed fish

“…A previous study in Turkey, 60 % of E. coli from cage culture O. mykiss (Onmaz et al, 2020) (Prakasan et al, 2022). Few previous reports in West Africa pathotypes of E. coli from farm O. niloticus were screened with different genes; with a percent prevalence of 8.33% Shiga toxigenic E. coli (STEC), 3.33% EIEC and 31% EHEC and 28% Shigella was isolated from market fish in Houston, Texas, USA (Okyer et al, 2018;Kouadio-Ngbesso et al, 2019). In a previous study, the pathogenic E. coli was revealed in O. niloticus and water with different virulence genes in Egypt (Galal et al, 2013).…”

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“…Recent work [5] on the waters in the ponds of this experimental farm revealed their strong contaminations with faecal coliforms and Vibrio. Our recent studies conducted [6], revealed the presence of enteropathogenic, toxigenic and enteroinvasive strains of E. coli in the gills and viscera of fish Oreochromis niloticus caught in Layo ponds, an aquaculture station. The presence of multiresistant germs in environment has also been detected [7,8].…”