Shiga toxin-producing bacteria as emerging enteric pathogens associated with outbreaks of foodborne illness in the Islamic Republic of Iran

Tajeddin, Elahe; Ganji, Leila; Hasani, Zahra; Mostafaei, Fahimeh Sadat Ghoalm; Azimirad, Masoumeh; Torabi, Parisa; Mohebbi, Seyed Reza; Aghili, Nooshin; Gouya, Mohammad Mehdí; Eshrati, Babak; Rahbar, Mohammad; Samiee, Siamak Mirab; Farzami, Marjan Rahnamaye; Zali, Mohammad Reza; Alebouyeh, Masoud

doi:10.26719/emhj.19.102

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…DEC pathotypes from different locations around the globe are genetically diverse (Yu et al, 2018). In outbreak situations, Vibrio cholerae, Escherichia coli and Clostridium difficile have been reported as the causative agents of diarrhoea in Bangkok (Dalsgaard et al, 1999), China (Scheutz et al, 2011;Escher et al, 2014), Yemen (Camacho et al, 2018;Weill et al, 2019), Iran (Tajeddin et al, 2019) and Nigeria (Eko et al, 1994;Lawoyin et al, 1999;Usman et al, 2005;Elimian et al, 2019). The role of viruses as agents of diarrhoea outbreaks among children cannot be overemphasized.…”

Section: Aetiology Of Diarrhoeal Diseasesmentioning

confidence: 99%

Childhood diarrhoeal diseases in developing countries

Ugboko

Nwinyi

Oranusi

et al. 2020

Heliyon

148

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Diarrhoeal diseases collectively constitute a serious public health challenge globally, especially as the leading cause of death in children (after respiratory diseases). Childhood diarrhoea affecting children under the age of five accounts for approximately 63% of the global burden. Accurate and timely detection of the aetiology of these diseases is very crucial; but conventional methods, apart from being laborious and time-consuming, often fail to identify difficult-to-culture pathogens. The aetiological agent of an average of up to 40% of cases of diarrhoea cannot be identified. This review gives an overview of the recent trends in the epidemiology and treatment of diarrhoea and aims at highlighting the potentials of metagenomics technique as a diagnostic method for enteric infections.

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Section: Aetiology Of Diarrhoeal Diseasesmentioning

confidence: 99%

Childhood diarrhoeal diseases in developing countries

Ugboko

Nwinyi

Oranusi

et al. 2020

Heliyon

148

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“…In the group of non– E. coli strains from wastewater samples, the prevalence of Shiga toxin genes stx 1 (38% of non– E. coli strains) and stx 2 (21% of non– E. coli strains) was also considerably higher than in E. coli strains from wastewater samples, where stx 1 was identified in 5% of the strains and stx 2 was detected in only 3% of the strains. Shiga toxins are produced mainly by E. coli and Shigella dysenteriae, but they are also synthesized by other bacteria of the family Enterobacteriaceae, including Citrobacter freundii , Enterobacter cloacae , and Shigella flexneri (Herold et al 2004 ; Tajeddin et al 2020 ). Shiga toxins are encoded by bacteriophages, which is why they are highly mobile and can be easily transferred between bacteria (Bai et al 2018 ).…”

Section: Resultsmentioning

confidence: 99%

The challenges in the identification of Escherichia coli from environmental samples and their genetic characterization

Osińska

Korzeniewska

Korzeniowska‐Kowal

et al. 2022

Environ Sci Pollut Res

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Escherichia coli bacteria are an essential indicator in evaluations of environmental pollution, which is why they must be correctly identified. This study aimed to determine the applicability of various methods for identifying E. coli strains in environmental samples. Bacterial strains preliminary selected on mFc and Chromocult media as E. coli were identified using MALDI Biotyper techniques, based on the presence of genes characteristic of E. coli (uidA, uspA, yaiO), as well as by 16S rRNA gene sequencing. The virulence and antibiotic resistance genes pattern of bacterial strains were also analyzed to investigate the prevalence of factors that may indicate adaptation to unsupportive environmental conditions and could have any significance in further identification of E. coli. Of the strains that had been initially identified as E. coli with culture-based methods, 36–81% were classified as E. coli with the use of selected techniques. The value of Cohen’s kappa revealed the highest degree of agreement between the results of 16S rRNA gene sequencing, the results obtained in the MALDI Biotyper system, and the results of the analysis based on the presence of the yaiO gene. The results of this study could help in the selection of more accurate and reliable methods which can be used in a preliminary screening and more precise identification of E. coli isolated from environmental samples.

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“…Moreover, using a different methodological approach, such as immunomagnetic concentration or chromogenic agars, could reveal additional STEC. Moreover, stx genes can be found in other E. coli (EPEC) and other bacterial species such as Citrobacter freundii , Shigella spp., among others which might explain part of these results [ 78 , 79 ]. However, it is important to clarify that this study was not designed to determine the actual non-O157 STEC prevalence in Chile; this study is a first approximation in assessing the problem of STEC in cattle in Chile, and further studies are necessary.…”

Section: Discussionmentioning

confidence: 97%

Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile

Díaz

Gutiérrez

Switt

et al. 2021

Animals

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Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes tet(A) and tet(R); no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (eae) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the stx2 gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes.

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Shiga toxin-producing bacteria as emerging enteric pathogens associated with outbreaks of foodborne illness in the Islamic Republic of Iran

Cited by 5 publications

References 19 publications

Childhood diarrhoeal diseases in developing countries

Childhood diarrhoeal diseases in developing countries

The challenges in the identification of Escherichia coli from environmental samples and their genetic characterization

Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile

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