Shiga toxin-producing Escherichia coli in beef retail markets from Argentina

Brusa, Victoria; Aliverti, Virginia; Aliverti, Florencia; Ortega, Emanuel E.; Torre, Julian Horacio de la; Linares, Luciano Héctor; Sanz, Marcelo E.; Etcheverría, Analía Inés; Padola, Nora Lía; Galli, Lucía; Peral‐García, Pilar; Copes, Julio Alberto; Leotta, Gerardo Aníbal

doi:10.3389/fcimb.2012.00171

Cited by 51 publications

(48 citation statements)

References 36 publications

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“…The results of the present study demonstrated that meat and meat products possess a high potential for causing human infection. Our findings indicated that 23.5% of minced beef samples were contaminated with STEC which was lower than that reported in previous investiga- tions in Iran (29.7%) and Argentina (52.5%) (Brusa et al, 2013) but higher than the result found in Switzerland (1.75%) and New Zealand (12%) (Brooks et al, 2001;Fantelli & Stephan, 2001). Considering the rising public health concern about non-O157 STEC, since 2012, Department of Agriculture (USDA)'s Food Safety and Inspection Service, expanded the zero tolerance policy for E. coli O157:H7 to include the top six non-O157 STEC serogroups in raw beef products (Wang et al, 2013).…”

Section: Discussioncontrasting

confidence: 76%

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

Panahee¹,

Pourtaghi²

2017

BJVM

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Shiga toxin-producing Escherichia coli (STEC) is recognised as toxin producing group of E. coli and one of the most significant foodborne pathogens worldwide. The aim of this study was to detect STEC and determine virulence gene profiles of these pathogens in different kinds of meat and products in Iran. For this reason a total of 182 samples of minced beef, mutton, chicken meat, chicken feet and mechanically separated chicken meat were collected from retail markets for detection of STEC by PCR method. Of the 72 E. coli isolated from examined samples, 29 of them were STEC. The highest presence of STEC was detected in minced beef (23.5%) followed by chicken feet (15%), mutton (13.3%), mechanically separated chicken meat (12.5%) and chicken meat (5.5%) respectively. In addition the results of PCR assay indicated that 21 (72.4%) and 4 (13.7%) of isolates carried stx2 and eaeA genes respectively. However, according to the results stx2 was the most prevalent gene detected in all kinds of examined samples. Our findings showed that evaluation of the prevalence and virulence factors of this pathogen seems necessary considering the increasing importance of STEC as one of the most significant foodborne pathogens.

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Section: Discussioncontrasting

confidence: 76%

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

Panahee¹,

Pourtaghi²

2017

BJVM

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“…Ground beef contamination with STEC is a leading cause of dysenteric diarrhea foodborne outbreaks and HUS (Rivas et al, 2006;Werber et al, 2012). Countries in Latin America and Europe, in addition to the United States, have reported ground beef contamination with STEC (Brusa et al, 2012;Soborg et al, 2013;Robbins et al, 2014).…”

mentioning

confidence: 99%

Genetic Relatedness Among Escherichia coli Pathotypes Isolated from Food Products for Human Consumption in Cartagena, Colombia

Amézquita-Montes

Tamborski

Kopsombut

et al. 2015

Foodborne Pathogens and Disease

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Foodborne pathogens are a leading cause of mild-to-severe gastrointestinal illnesses worldwide. Escherichia coli pathotypes have been known to cause gastrointestinal illnesses in children less than 5 years old in Colombia. However, insufficient information is available on the prevalence of E. coli contamination of food products and the kind of E. coli food product reservoirs. The two objectives of this study were designed to address this issue. The first objective was to ascertain coliform, E. coli, and pathogenic E. coli contamination of food products readily available for human consumption in Cartagena, Colombia. The second objective was to evaluate the relationship between pathogenic E. coli isolated from food products and those isolated from cases of diarrhea in children. Food product samples consisting of pasteurized milk, unpasteurized fruit juice, ground beef, cheese, and vegetables were obtained at four retail stores. The food samples were cultured in liquid media and tested for the presence of coliforms and E. coli. E. coli isolates were tested by polymerase chain reaction for the presence of pathogenic E. coli. Coliforms, E. coli, and E. coli intestinal pathotypes contamination were detected in 88.4%, 53%, and 2.1% of food product samples, respectively. Ground beef and cheese were the only food samples contaminated with E. coli intestinal pathotypes including enteropathogenic (EPEC), Shiga toxinproducing (STEC), and enterotoxigenic E. coli (ETEC). Closed multilocus sequencing typing relationships between diarrheagenic E. coli isolates from food products and from individuals with diarrhea suggest that food products readily available at public markets in Cartagena can transmit ETEC and possibly EPEC and STEC. We demonstrated that a high proportion of food products for human consumption available at public markets in Cartagena are contaminated with coliforms, E. coli, and E. coli intestinal pathogens. Furthermore, food products containing E. coli intestinal pathogens may be involved in the transmission of foodborne illnesses among children in Cartagena, Colombia.

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“…A total of 20 STEC O157:H7 strains isolated in previous studies from butcher shops were studied (Brusa et al, 2012;Brusa et al, 2015). The samples were collected between 2010 and 2013 from the city of Berisso, which has 135 km 2 and 83,123 inhabitants.…”

Section: Bacterial Strainsmentioning

confidence: 99%

High prevalence of clade 8 Escherichia coli O157:H7 isolated from retail meat and butcher shop environment

Galli

Brusa

Singh

et al. 2016

Infection, Genetics and Evolution

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Escherichia coli O157:H7 is an enteric pathogen associated with food safety threats and with significant morbidity and mortality worldwide. In Argentina, post-enteric hemolytic uremic syndrome (HUS) is endemic, with more than 70% of cases associated with E. coli O157 infection. To date the biological basis behind the severity among E. coli O157 infections is unknown. However, single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades, of which clade 8 strains are associated with severe disease cases. The aim of this study was to characterize a collection of 20 STEC O157:H7 strains isolated between 2011 and 2013 from ground beef and different environmental samples from butcher shops of Argentina. All strains harbored the eae, ehxA, fliC H7 , efa, iha, and toxB genes, with stx 2a /stx 2c as the predominant genotype (75%). The XbaI-PFGE analysis showed that the E. coli O157 strains had high genetic diversity. Nine strains were grouped in four XbaI-PFGE clusters, whereas 11 strains showed unique XbaI-PFGE patterns. In contrast, the SNP analysis allowed us to separate the strains in two distinct lineages representing clade 8 (70%) and clade 6 (30%). Our results show the molecular characterization of E. coli O157 strains isolated from ground beef and environmental samples from Argentinean butcher shops.

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Shiga toxin-producing Escherichia coli in beef retail markets from Argentina

Cited by 51 publications

References 36 publications

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

Virulence gene profiles of Shiga-toxin producing Escherichia coli isolates from retail raw meat in Iran.

Genetic Relatedness Among Escherichia coli Pathotypes Isolated from Food Products for Human Consumption in Cartagena, Colombia

High prevalence of clade 8 Escherichia coli O157:H7 isolated from retail meat and butcher shop environment

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