Shiga-toxin-producing Escherichia coli in faeces of healthy dairy cows, sheep and goats: prevalence and virulence properties

Zschöck, M.; Hamann, Hans-Peter; Kloppert, B.; Wolter, W.

doi:10.1046/j.1365-2672.2000.00789.x

Cited by 106 publications

(83 citation statements)

References 29 publications

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“…In different studies on STECs isolated from sheep and goats, this pathotype mostly do not carry stx2 and eae (28,(35)(36)(37)(38). Oliveira et al (32) reported lacking of eae gene among the STECs from ruminants which is almost in agreement with our observation in goats.…”

Section: Discussionsupporting

confidence: 83%

Distribution Pattern of EcoR Phylogenetic Groups Among Shiga Toxin-Producing and Enteropathogenic Escherichia coli Isolated From Healthy Goats

Jajarmi¹,

Ghanbarpour²,

Sharifi³

et al. 2015

Int J Enteric Pathog

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Background: Escherichia coli can be categorized into four major phylogenetic groups (A, B1, B2, and D) based on presence or absence of three markers including two genes (chuA and yjaA) and an anonymous DNA fragment designated TSPE4.C2. Also, these groups are divided into seven phylogenetic subgroups A 0 , A 1 , B1, B2 2 , B2 3 , D 1 , and D 2 . Objectives: This study aimed to determine the distribution pattern of phylogenetic groups in Shiga Toxin-Producing Escherichia coli (STEC) and Enteropathogenic Escherichia coli (EPEC) isolated from asymptomatic goats in Kerman city, Iran. Materials and Methods: Two hundred and fifty fecal samples were obtained from healthy goats. All isolates were subjected to detection of phylogenetic markers chuA, yjaA and DNA fragment TspE4.C2 and virulence genes stx1, stx2 and eae. Results: In summary, among all isolates phylo-group B1 was the most prevalent (57.6%) and other phylo-groups were A 1 (20.4%), A 0 (18.4%), D 1 (2.8%), and B2 2 (0.8%). There was no isolate in B2 3 and D 2 subgroups. Fifty samples (20%) possessed at least one of the tested virulence genes: stx1 (12%), stx1/stx2 (4%), eae (2.8%), stx1/eae (0.8%), and stx2 (0.4%). Thus, 41 (16.4%) STEC, 7 (2.8%) EPEC, and 2 (0.8%) Enterohemorrhagic Escherichia coli (EHEC) strains were isolated and allocated into four phylogenetic subgroups A 0 (16%), A 1 (12%), B1 (68%), and D 1 (4%). Conclusions: Based on 250 fecal samples obtained from goats in industrial slaughterhouse of Kerman City, goats may be a potential reservoir of STEC in Kerman and B1 followed by A are the most prevalent phylogenetic groups among STEC and non-STEC isolates in this study.

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Section: Discussionsupporting

confidence: 83%

Distribution Pattern of EcoR Phylogenetic Groups Among Shiga Toxin-Producing and Enteropathogenic Escherichia coli Isolated From Healthy Goats

Jajarmi¹,

Ghanbarpour²,

Sharifi³

et al. 2015

Int J Enteric Pathog

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“…The frequency of the bacteria shedding in cattle is influenced by various factors (Dargatz et al, 1997;Hancock et al, 1997a,b;Cray et al, 1998). STEC shedding has also been demonstrated in small ruminants (Beutin et al, 1993(Beutin et al, , 1996aKudva et al, 1996;Zschock et al, 2000), and that may represent an equally serious risk for people as cattle infections as evidenced by a recent finding of STEC O157 in sheep milk in Supported by the Grant Agency of the Czech Republic (Grant No. 525/00/666).…”

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confidence: 99%

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7

Novotná¹,

Alexa²,

Hamrik³

et al. 2005

Vet. Med. - Czech

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Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordan were taken and examined for Shiga toxin-producing Escherichia coli (STEC). The bacteriological examination included the preenrichment of rectal swabs in EC broth with novobiocin, and a subsequent parallel isolation on enterohemolysin agar and immunomagnetic separation with cultivation on CT-SMAC. The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1-to 3-weeks old in one sheep herd with intensive milk production. In the remaining three herds, serogroups O128, O78, O15 and serotype O128:K85 of STEC strains were the most frequent findings. The presence of stx2, ehlyA and eaeA genes in all STEC O157:H7 isolates was confirmed by PCR. In two untypable STEC isolates, stx2 and ehlyA genes were detected. In other STEC non-O157 isolates, only stx1 a ehlyA genes were found. All STEC O157:H7 isolates were resistant against sulphonamides and chloramphenicol, five were also resistant against ampicillin and streptomycin, one against co-trimoxazole. One isolate was resistant against ampicillin, ampicillin-sulbactam, cephalosporins (cefazolin, cefuroxime), monobactams (aztreonam), sulphonamides, co-trimoxazole, aminoglycosides, tetracycline and chloramphenicol. Compared the resistant STEC O157:H7 isolates, the multiresistant isolate had a different RAPD pattern. Of 36 STEC non-O157 isolates, one isolate was resistant against sulphonamides and co-trimoxazole, and another one against ampicillin, streptomycin, sulphonamides and co-trimoxazole. STEC isolates resistant against antimicrobial agents were demonstrated only in herds with intensive management.

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“…This contributes to diminishing the environmental pollution from manure disposal (11). However, animal manure is a well-known source of foodborne pathogenic bacteria (32,33,35,37,46,51,61,63,65), and its inappropriate use in vegetable crops, especially organic ones, contributes a risk to consumer health. Research data and regulations recommend evaluating and normalizing the use of animal manure at the agricultural production (20,21).…”

Section: Introductionmentioning

confidence: 99%

Microbiological quality of organic vegetables produced in soil treated with different types of manure and mineral fertilizer

Machado

Maia

Carvalho

et al. 2006

Braz. J. Microbiol.

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An attempt was made to evaluate microbiological quality of horticultural crops grown organically. Three species of vegetables were used, lettuce (Lactuva sativa), radish (Raphanus sativus) and spinach (Tetragonia expansa), grown organically, in fertile soil. Six different treatments were applied: mineral fertilizer, chicken, cow, and pig manure, chicken litter and cow manure, in association with a liquid foliar biofertilizer. These crops were handled according to correct agronomic practices for growing crops organically for commercial purposes. Samples were examined for the Most Probable Number (MPN/g/mL) of total and fecal coliforms and to detect the presence of Escherichia coli and Salmonella spp. All analyzed samples were considered acceptable for consumption, as Salmonella spp. was not detected. However, 63.3%, 50.0%, and 23.3% of the samples of lettuce, radish and spinach, respectively, contained ≥10 2 total coliforms/g of product. None of the samples of spinach or radish presented ≥10 2 fecal coliforms/g, and only 6.6% of lettuce samples contained >10 2 fecal coliforms/g. The presence of E. coli was confirmed in one sample of spinach, cultivated with cow manure.

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Shiga-toxin-producing Escherichia coli in faeces of healthy dairy cows, sheep and goats: prevalence and virulence properties

Cited by 106 publications

References 29 publications

Distribution Pattern of EcoR Phylogenetic Groups Among Shiga Toxin-Producing and Enteropathogenic Escherichia coli Isolated From Healthy Goats

Distribution Pattern of EcoR Phylogenetic Groups Among Shiga Toxin-Producing and Enteropathogenic Escherichia coli Isolated From Healthy Goats

Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7

Microbiological quality of organic vegetables produced in soil treated with different types of manure and mineral fertilizer

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