Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh

Islam, Md. Atikul; Heuvelink, Annet; Boer, E de; Sturm, Patrick; Beumer, R.R.; Zwietering, Marcel H.; Faruque, A. S. G.; Haque, Rashidul; Sack, David A.; Talukder, Kaisar A.

doi:10.1099/jmm.0.46916-0

Cited by 52 publications

(52 citation statements)

References 36 publications

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“…The second multiplex PCR was carried out for Shiga toxin genes (stx 1 and stx 2 ) according to the procedure described earlier. 17 PCR was performed according to published procedures to demonstrate the presence of invasion associated locus (ial) and the invasion plasmid antigen H (ipaH). 18,19 Reactions were performed in a thermal cycler (Gene Amp PCR system 9700, Applied Biosystems, Foster City, CA, USA) and amplification products were electrophoresed on a 1% agarose gel in Tris-borate EDTA buffer.…”

Section: Bacteriological Analysis Of Cf Samplesmentioning

confidence: 99%

Microbiological quality of complementary foods and its association with diarrhoeal morbidity and nutritional status of Bangladeshi children

et al. 2012

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BACKGROUND/OBJECTIVES:To examine the bacteriological quality of complementary foods (CF) and to correlate the results with diarrhoeal morbidity and nutritional status of Bangladeshi children aged 6-24 months. SUBJECTS/METHODS: A total of 212 CF samples were tested, of which 72 were collected immediately before the first time of feeding (p1 h of food preparation) and 140 were collected at second/third time of feeding from 140 households located in urban and rural areas of Bangladesh. Anthropometry, food frequency data and demographic information of the children were collected. RESULTS: Of the first time feeding samples, 3% from each of urban and rural areas were found to be contaminated with faecal coliforms (FC) at X100 CFU/g. E. coli was isolated from 11% and 6% of samples, and B. cereus from 8% and 6% of samples from urban and rural areas, respectively. In contrast, 33% of the second/third time feeding samples from urban areas and 19% from rural areas were contaminated with FC at X100 CFU/g (Po0.05). E. coli was isolated from 40% and 39% of samples, and B. cereus from 33% and 26% of samples from urban and rural areas, respectively. Significantly high numbers of wasted rural children had CF with a high aerobic plate count, which was also significantly associated with diarrhoeal morbidity in children. CONCLUSIONS: Around 40% of CF samples were contaminated with E. coli, which was mainly attributable to food preparation practices. Consumption of contaminated CF appeared to be associated with a higher frequency of diarrhoea and malnutrition in children.

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Section: Bacteriological Analysis Of Cf Samplesmentioning

confidence: 99%

Microbiological quality of complementary foods and its association with diarrhoeal morbidity and nutritional status of Bangladeshi children

et al. 2012

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“…Bangladesh is an endemic zone for diarrheal diseases and in recent years, STEC non-O157:H7 has also been isolated from hospitalized patients. Although the STEC non-O157:H7 is an uncommon pathogen among the hospitalized patients with diarrhea, however, the low prevalence of STEC non-O157:H7 in Bangladesh might be due to acquired immunity against the pathogen in the population (Islam et al, 2007). Apart from the few reports on the isolation and virulence study of STEC O157:H7 from slaughtered animals, no STEC O157:H7 has been isolated either from diarrheal patients or other sources in Bangladesh (Islam et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…In fact, most of the outbreaks were more often reported from the industrialized countries than from developing countries because of the advanced surveillance and reporting systems in the industrialized countries. However, there are few reports on the prevalence of the Shiga-toxin producing E. coli (STEC) in Bangladesh (Islam et al, 2007(Islam et al, , 2008(Islam et al, , 2010. All these reports included the isolation and molecular characterizations of these organisms from the diarrheal patients, slaughtered animals, raw meat and other food samples.…”

Section: Introductionmentioning

confidence: 99%

Bovine Shiga toxin producing Escherichia coli O157:H7 of Bangladesh: Is it capable of causing diseases similar to clinical strains?

Rabbi¹,

Yasmin²,

Nessa³

et al. 2014

Afr. J. Microbiol. Res.

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Escherichia coli O157:H7 is a predominant serotype of Shiga toxin producing E. coli (STEC) and is responsible for many outbreaks worldwide. Until recently, there is no comparative study on the bovine and clinical isolates as no STEC O157:H7 has been isolated from patients in Bangladesh. In the present study, the local bovine isolates were compared with a reference clinical strain to investigate whether the bovine isolates are capable of producing same degree of illness as the clinical strain. Two local isolates (CD-11 and CD-17) of bovine origin and a reference clinical strain (E. coli O157:H7 NCTC 12079) were investigated for the presence of virulence genes by polymerase chain reaction (PCR), Shiga toxin production by VTEC-RPLA, invasive property by Congo red binding and Sereny's keratoconjunctivitis, and other enterotoxic, cytotoxic and mouse lethal activities. Both the local isolates and the reference clinical strain showed the presence of eae and stx 2 genes and were found to be non-invasive. The isolates also produced enterotoxin, cytotoxin and mouse lethality similar to that found with the reference strain. All these results strongly suggest that the local bovine STEC O157:H7 isolates have potential to cause diseases similar to clinical STEC O157:H7 strain, which might lead to any outbreak in Bangladesh.

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“…Whereas infection prevalence was as high as 14-39.6z in India and Iran due to geographic characteristics, environmental conditions, animal population in the area, and contamination of food and water sources (6,28), low rates were observed (0.87-2.5z) in Bangladesh and Spain (29,30). Data is scarce in Turkey with the reported prevalence being 0-1.2z for O157:H7 strains (31,32).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Humans between 2011 and 2014

Güleşen¹,

Levent²,

Demir

et al. 2016

Jpn J Infect Dis

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SUMMARY: Although E. coli O157:H7 is the major serotype among Shiga toxin-producing Escherichia coli (STEC) strains, non-O157 serotypes have caused numerous outbreaks worldwide. We aimed to evaluate the distribution of serogroups, serotypes, virulence genes, and antimicrobial resistance of STEC strains recovered from stool samples. A total of 395 stool samples characterized by watery/bloody diarrhea and/or symptoms of hemolytic-uremic syndrome were included in this study. Strains compatible with E. coli, based on biochemical tests, were tested for the presence of Shiga toxin by ELISA. Toxigenic strains were tested by serotyping and serogrouping. Virulence genes, stx1, stx2, aggR, hlyA, and eae were detected by polymerase chain reaction. Overall, 26 (6.6z) stool culture samples tested positive for STEC. Shiga toxin was positive in 28 (7.1z) patient isolates based on ELISA and PCR. Two isolates could not be serotyped. STEC strains were distributed into 10 serogroups and 14 serotypes. Of the serotyped strains, 92.3z were non-O157, with the major distribution in O104:H4 and O26:HNM. All were negative for extended-spectrum b-lactamase enzyme and 62.5z were resistant to at least 1 drug. This study demonstrated the wide distribution of non-O157 STEC strains from our patient group. Further studies should be performed to better understand STEC characteristics on a larger scale.

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Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh

Cited by 52 publications

References 36 publications

Microbiological quality of complementary foods and its association with diarrhoeal morbidity and nutritional status of Bangladeshi children

Microbiological quality of complementary foods and its association with diarrhoeal morbidity and nutritional status of Bangladeshi children

Bovine Shiga toxin producing Escherichia coli O157:H7 of Bangladesh: Is it capable of causing diseases similar to clinical strains?

Characterization of Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Humans between 2011 and 2014

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