Shipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days

El-Shalofy, Amr S.; Gautier, Camille; Khan, Yasmeen; Aurich, Jörg; Aurich, Christine

doi:10.1016/j.anireprosci.2023.107307

Cited by 2 publications

(1 citation statement)

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“…Acrosomal status and membrane integrity are another fundamental parameter of spermatozoa quality assessment tracked in our study. Studies have shown that transferring frozen horse semen from LN to dry ice for 7 days and then transferring it to LN had no adverse effect on its post-thaw characteristics [27]. However, some experiments have shown [4] that placing human semen in a −80 • C refrigerator can lead to a decrease in motility and vitality after thawing.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen

He,

Sun,

et al. 2024

Animals

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To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (−79 °C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 °C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN (p > 0.05). There were no significant changes in antioxidant properties between storage groups either (p > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.

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Section: Discussionmentioning

confidence: 99%