2019
DOI: 10.1111/1346-8138.14810
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Shiunko and Chuoko, topical Kampo medicines, inhibit the expression of gehA encoding the extracellular lipase in Cutibacterium acnes

Abstract: Antimicrobial agents have been used for eradication of Cutibacterium (formerly Propionibacterium) acnes that is an exacerbation factor of the skin disease acne vulgaris. However, the use of antibiotics is associated with an increased risk of promoting the emergence of resistant bacteria and leading to skin dysbiosis. Traditional Japanese Kampo medicines, such as Keigairengyoto, are used to treat acne. However, there is incomplete understanding regarding their functional mechanism in treatment of acne. In this … Show more

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Cited by 9 publications
(9 citation statements)
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“…Total RNA was extracted from C. acnes using a Blood/Cultured Cell Total RNA Mini Kit (Favorgen Biotech Co., Ping-Tung, Taiwan). 14 Reverse transcription and reverse transcription quantitative PCR (RT-qPCR) were performed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo, Osaka, Japan) and THUNDERBIRD™ SYBR qPCR MIX (Toyobo). The following specific primers were used for the analysis of tet(W) and internal standard gene oxc (cytochrome c oxidase subunit II): tet(W)-rtF (5′-CCGACAGCAAAGTGGAAACAA), tet(W)-rtR (5′-GCAACTTGCGGATACTGACC), oxc-rtF (5′-CTTG TCATCGGCGTATTCG), and oxc-rtR (5′-CACTTCAAGCGGAAGGT GA).…”
Section: Analysis Of Upstream Sequence and Mrna Expression Of Tet(w)mentioning
confidence: 99%
“…Total RNA was extracted from C. acnes using a Blood/Cultured Cell Total RNA Mini Kit (Favorgen Biotech Co., Ping-Tung, Taiwan). 14 Reverse transcription and reverse transcription quantitative PCR (RT-qPCR) were performed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo, Osaka, Japan) and THUNDERBIRD™ SYBR qPCR MIX (Toyobo). The following specific primers were used for the analysis of tet(W) and internal standard gene oxc (cytochrome c oxidase subunit II): tet(W)-rtF (5′-CCGACAGCAAAGTGGAAACAA), tet(W)-rtR (5′-GCAACTTGCGGATACTGACC), oxc-rtF (5′-CTTG TCATCGGCGTATTCG), and oxc-rtR (5′-CACTTCAAGCGGAAGGT GA).…”
Section: Analysis Of Upstream Sequence and Mrna Expression Of Tet(w)mentioning
confidence: 99%
“…Lipase activity was measured using the method reported by Nakase et al [ 10 ]. 4-Methylumbelliferyl oleate (4-MUO, Sigma-Aldrich, St. Louis, USA) was used as a lipase fluorescent substrate, and fluorescence intensity was measured using Varioskan Flash 2.4 (Thermo, MS, USA) with an excitation filter of 320 nm and an emission filter of 450 nm.…”
Section: Methodsmentioning
confidence: 99%
“…4-Methylumbelliferyl oleate (4-MUO, Sigma-Aldrich, St. Louis, USA) was used as a lipase fluorescent substrate, and fluorescence intensity was measured using Varioskan Flash 2.4 (Thermo, MS, USA) with an excitation filter of 320 nm and an emission filter of 450 nm. The expression of the gehA gene encoding extracellular lipase was analysed using the method reported by Nakase et al [ 10 ]. After anaerobic incubation of C. acnes ATCC11828 for 72 hr, total RNA was extracted using a High Pure RNA Isolation Kit (Roche).…”
Section: Methodsmentioning
confidence: 99%
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