Shoaling, boldness, anxiety-like behavior and locomotion in zebrafish (Danio rerio) are altered by acute benzo[a]pyrene exposure

Hamilton, Trevor J.; Krook, Jeffrey; Szaszkiewicz, Joshua; Burggren, Warren W.

doi:10.1016/j.scitotenv.2021.145702

Cited by 53 publications

(23 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The open field test is a commonly used paradigm in behavioural research to assess zebrafish anxiety-like behaviour and locomotion 30 , 39 , 42 . In this test, anxiety-like behaviour is quantified as time spent in the various ‘virtual’ zones of the arena (thigmotaxis, transition, and center zone), with more time being spent near the walls of the arena in the thigmotaxis zone being indicative of anxiety-like behaviour.…”

Section: Methodsmentioning

confidence: 99%

“…The novel object approach test is another well-validated behavioural assay used to assess boldness and anxiety-like behaviour 30 , 35 , 37 , 39 . Immediately after an open field test trial, a novel object was placed directly in the center of the arena and motion-tracking recording was initiated via EthoVision.…”

Section: Methodsmentioning

confidence: 99%

“…Locomotion and anxiety-like behaviour were assessed by the open field test followed by the novel object approach test which quantified boldness behaviour. The open field and novel object approach test have been used extensively in behavioural neuroscience research and are well-validated measures of anxiety-like behaviour and boldness 30 , 38 , 39 . Additionally, in order to examine the differences between short-term and long-term effects of the terpenes investigated, we exposed zebrafish to repeated terpene treatment for seven consecutive days and immediately afterward assessed their behaviour in the open field and novel object approach test.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)

Szaszkiewicz

Leigh

Hamilton

2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Terpenes are fragrant aromatic compounds produced by a variety of plants, most notably cannabis and hops. With increasing legalization of cannabis there is a need to better understand the behavioural effects of terpenes and ultimately their therapeutic value. Our study investigated the dose-dependent impact of three terpenes (limonene 0.25, 0.5, 0.75%; β-myrcene 0.001, 0.01, 0.1%; and 0.0001, 0.001, 0.00125% linalool) on zebrafish (Danio rerio) behaviour when exposed both acutely and repeatedly over a 7-day period. Anxiety-like behaviour, boldness, and locomotion were assessed using the open field test and the novel object approach test. In the acute dosing experiment, limonene and β-myrcene exposed groups demonstrated a significant decrease in locomotion, a decrease in anxiety-like behaviour, and an increase in boldness, while linalool treatment groups demonstrated only minor alterations in locomotion. Moreover, repeated exposure to limonene (0.39%) or β-myrcene (0.0083%) for a seven day period did not result in any significant behavioural effects. In conclusion, our study provides support for an anxiolytic and sedative effect in zebrafish in response to acute limonene and β-myrcene exposure that is no longer present after one week of repeated exposure.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)

Szaszkiewicz

Leigh

Hamilton

2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The open eld test is a commonly used paradigm in behavioural research to assess zebra sh anxiety-like behaviour and locomotion 30,39,42 . In this test, anxiety-like behaviour is quanti ed as time spent in the various 'virtual' zones of the arena (thigmotaxis, transition, and center zone), with more time being spent near the walls of the arena in the thigmotaxis zone being indicative of anxiety-like behaviour.…”

Section: Open Eld Testmentioning

confidence: 99%

“…Locomotion and anxietylike behaviour were assessed by the open eld test followed by the novel object approach test which quanti ed boldness behaviour. The open eld and novel object approach test have been used extensively in behavioural neuroscience research and are well-validated measures of anxiety 30,38,39 . Additionally, in order to examine the differences between short-term and long-term effects of the terpenes investigated, we exposed zebra sh to repeated terpene treatment for seven consecutive days and immediately afterward assessed their behaviour in the open eld and novel object approach test.…”

Section: Introductionmentioning

confidence: 99%

Robust Behavioural Effects in Response to Acute, But Not Repeated, Terpene Administration in Zebrafish (Danio Rerio)

Szaszkiewicz

Leigh

Hamilton

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Terpenes are fragrant aromatic compounds produced by a variety of plants, most notably cannabis and hops. With increasing legalization of recreational and medicinal cannabis there is growing interest to better understand the behavioural effects of terpenes and ultimately their therapeutic value. Our study investigated the dose-dependent impact of three terpenes (limonene 0.25, 0.5, 0.75%; β-myrcene 0.001, 0.01, 0.1%; and 0.0001, 0.001, 0.00125% linalool) on zebrafish (Danio rerio) behaviour when exposed both acutely and repeatedly over a 7-day period. Anxiety-like behaviour, boldness, and locomotion were assessed using the open field test and the novel object approach test. In the acute dosing experiment, limonene and β-myrcene treatment groups demonstrated a significant decrease in locomotion, a decrease in anxiety-like behaviour, and an increase in boldness, while linalool treatment groups had minor alterations in locomotion. Moreover, chronic exposure to limonene (0.39%) or β-myrcene (0.0083%) did not result in any significant behavioural effects, suggesting that anxiety-like behaviour, locomotion, and boldness were not impacted at these levels. In conclusion, our study provides support for an anxiolytic and sedative effect in zebrafish in response to acute limonene, β-myrcene, and linalool exposure.

show abstract

Environmental enrichment decreases anxiety‐like behavior in zebrafish larvae

Gatto

Dadda

Bruzzone

et al. 2022

Developmental Psychobiology

View full text Add to dashboard Cite

The development of anxiety disorders is often linked to individuals’ negative experience. In many animals, development of anxiety‐like behavior is modeled by manipulating individuals’ exposure to environmental enrichment. We investigated whether environmental enrichment during early ontogenesis affects anxiety‐like behavior in larval zebrafish. Larvae were exposed from hatching to either an environment enriched with 3D‐objects of different color and shape or to a barren environment. Behavioral testing was conducted at different intervals during development (7, 14, and 21 days post‐fertilization, dpf). In a novel object exploration test, 7 dpf larvae of the two treatments displayed similar avoidance of the visual stimulus. However, at 14 and 21 dpf, larvae of the enriched environment showed less avoidance, indicating lower anxiety response. Likewise, larvae of the two treatments demonstrated comparable avoidance of a novel odor stimulus at 7 dpf, with a progressive reduction of anxiety behavior in the enriched treatment with development. In a control experiment, larvae treated before 7 dpf but tested at 14 dpf showed the effect of enrichment on anxiety, suggesting an early determination of the anxiety phenotype. This study confirms a general alteration of zebrafish anxiety‐like behavior due to a short enrichment period in first days of life.

show abstract

Shoaling, boldness, anxiety-like behavior and locomotion in zebrafish (Danio rerio) are altered by acute benzo[a]pyrene exposure

Cited by 53 publications

References 78 publications

Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)

Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)

Robust Behavioural Effects in Response to Acute, But Not Repeated, Terpene Administration in Zebrafish (Danio Rerio)

Environmental enrichment decreases anxiety‐like behavior in zebrafish larvae

Contact Info

Product

Resources

About