Shock wave-induced permeabilization of mammalian cells

López-Marı́n, Luz M.; Rivera, Ana Leonor; Fernández, Francisco; Loske, Achim M.

doi:10.1016/j.plrev.2018.03.001

Cited by 37 publications

(23 citation statements)

References 408 publications

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“…If this is indicative of microbubble cavitation in vasculature, then the sustained mechanical activity in contact with the endothelial layer at higher PNP amplitudes could explain the more aggressive, often irreversible, bio-effects associated with subharmonic emissions (O'Reilly and Hynynen 2012; Jones et al 2018). Beyond physical contact, the role of bubble collapse shock waves for tissue disruption is a topic of ongoing research, with a thorough review recently available (Lopez-Marin et al 2018). The directivity of the 0.2-mm needle hydrophone used, and its close proximity to the capillary, mean the peakpositive pressure amplitudes of the shock wave measurements reported are sensitive to the precise bubble location, relative to the needle tip, including as a result of translation of the activity during the focused ultrasound exposure.…”

Section: Discussionmentioning

confidence: 99%

Non-linear Acoustic Emissions from Therapeutically Driven Contrast Agent Microbubbles

Song

Moldovan

Prentice

2019

Ultrasound in Medicine & Biology

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Non-linear emissions from microbubbles introduced to the vasculature for exposure to focused ultrasound are routinely monitored for assessment of therapy and avoidance of irreversible tissue damage. Yet the bubble-based mechanistic source for these emissions, under subresonant driving at typical therapeutic pressure amplitudes, may not be well understood. In the study described here, dual-perspective high-speed imaging at 210,000 frames per second (fps), and shadowgraphically at 10 Mfps, was used to observe cavitation from microbubbles flowing through a 500-mm polycarbonate capillary exposed to focused ultrasound of 692 kHz at therapeutically relevant pressure amplitudes. The acoustic emissions were simultaneously collected via a broadband calibrated needle hydrophone system. The observations indicate that periodic bubble-collapse shock waves can dominate the non-linear acoustic emissions, including subharmonics at higher driving amplitudes. Contributions to broadband emissions through variance in shock wave amplitude and emission timings are also identified. Possible implications for in vivo microbubble cavitation detection, mechanisms of therapy and the conventional classification of cavitation activity as stable or inertial are discussed. (

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Section: Discussionmentioning

confidence: 99%

Non-linear Acoustic Emissions from Therapeutically Driven Contrast Agent Microbubbles

Song

Moldovan

Prentice

2019

Ultrasound in Medicine & Biology

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“…Foglietta et al reported that when mesenchymal stem cells and cancer cells (glioblastoma or osteosarcoma) co-cultures are irradiated with SW, the consequence is a selective cancer cell death related to the ROS production by mesenchymal stem cells [ 163 ]. Otherwise, SW could be employed to permeabilize temporarily cell membrane and improve the uptake of drugs and chemotherapies [ 164 , 165 ], as well for the delivery of nucleic acids [ 166 , 167 ].…”

Section: Mechanical Wave-based Therapiesmentioning

confidence: 99%

Remotely Activated Nanoparticles for Anticancer Therapy

Racca

Cauda

2020

Nano-Micro Lett.

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Cancer has nowadays become one of the leading causes of death worldwide. Conventional anticancer approaches are associated with different limitations. Therefore, innovative methodologies are being investigated, and several researchers propose the use of remotely activated nanoparticles to trigger cancer cell death. The idea is to conjugate two different components, i.e., an external physical input and nanoparticles. Both are given in a harmless dose that once combined together act synergistically to therapeutically treat the cell or tissue of interest, thus also limiting the negative outcomes for the surrounding tissues. Tuning both the properties of the nanomaterial and the involved triggering stimulus, it is possible furthermore to achieve not only a therapeutic effect, but also a powerful platform for imaging at the same time, obtaining a nano-theranostic application. In the present review, we highlight the role of nanoparticles as therapeutic or theranostic tools, thus excluding the cases where a molecular drug is activated. We thus present many examples where the highly cytotoxic power only derives from the active interaction between different physical inputs and nanoparticles. We perform a special focus on mechanical waves responding nanoparticles, in which remotely activated nanoparticles directly become therapeutic agents without the need of the administration of chemotherapeutics or sonosensitizing drugs.

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“…In contrast to this work, previous studies only looked at ultrasonic sonoporation caused by cavitation [5]. Of note, rESWT has no heating effects [23], making a desirable feature for clinical use. These features together endow rESWT a safer and more convenient clinical therapy than ultrasound from the perspective of combinatory nanomedicine in cancer treatment.…”

Section: Discussionmentioning

confidence: 87%

“…Under this setting, rESWT-induced endothelial leakiness should supposedly favor the delivery and extravasation of nano-drugs into the tumor microenvironment and thereby reinforce tumor targeting and the following treatment. In fact, recent burgeoning evidence indicates that extracorporeal shock wave (ESW) is applicable to treat tumors, due to increased plasma membrane permeability and intracellular drug concentration that are ascribed to ESW-driven cavitation [21][22][23]. Thus, it would be of proven importance to extend ESW applications in cancer therapeutics, although a wider caliber of combinatory therapies and deeper understanding of molecular mechanisms are warranted.…”

Section: Introductionmentioning

confidence: 99%