Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.)

Ahmad, Abdullah; Zhong, Heng; Wang, Wengling; Sticklen, Mariam B.

doi:10.1079/ivp2001267

Cited by 56 publications

(30 citation statements)

References 22 publications

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“…In vitro culture of plant cells and tissue has attracted considerable interest over recent years because it provides the means to study plant physiological and genetic processes in addition to offering the potential to assist in the breeding of improved cultivars by increasing genetic variability [1]. In wheat species, different explant sources have been used for embryogenic callus formation and plant regeneration: mature and immature embryos [2,3], infloroscences [4,5], coleoptile [5], shoot apical meristems [6], and anthers [7]. These tissues vary in their ability to regenerate whole plants [8].…”

Section: Introductionmentioning

confidence: 99%

Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivumL.) Genotypes under Saline and Heat Stress Conditions

et al. 2012

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Response of two genotypes of bread wheat (Triticum aestivum), Mahon-Demias (MD) and Hidhab (HD1220), to mature embryo culture, callus production, and in vitro salt and heat tolerance was evaluated. For assessment of genotypes to salt and heat tolerance, growing morphogenic calli were exposed to different concentrations of NaCl (0, 5, 10, and 15 g·L −1 ) and under different thermal stress intensities (25, 30, 35, and 40 • C). Comparison of the two genotypes was reported for callus induction efficiency from mature embryo. While, for salt and heat tolerance, the proliferation efficiency, embryonic efficiency, and regeneration efficiency were used. The results show significant medium and genotype effects for the embryogenesis capacity of calluses induction and plantlets regeneration under saline and thermal stresses. Mahon-Demias showed good callus induction and ability to proliferate and regenerate seedling under heat and salt stress conditions compared to Hidhab. No sizeable differences were observed between the two genotypes at higher salt stress rates. This study will serve as a base line for in vitro screening of several elite wheat cultivars for their ability to induce callus and regenerate plants from mature embryos, and to start selection for tolerance to salinity.

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Section: Introductionmentioning

confidence: 99%

Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivumL.) Genotypes under Saline and Heat Stress Conditions

et al. 2012

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“…A system to produce multiple shoot primordia from cereal shoot apical meristems was developed in our laboratory (Zhong et al 1992(Zhong et al , 1998Zhang et al 1996;Devi et al 2000;Ahmad et al 2002). The efficient recovery of transgenic maize plants via this system suggested that this apical meristem system could be extended to develop a highly efficient and reproducible system for oat transformation .…”

Section: Introductionmentioning

confidence: 99%

Competence of oat (Avena sativa L.) shoot apical meristems for integrative transformation, inherited expression, and osmotic tolerance of transgenic lines containing hva1

et al. 2002

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Three oat ( Avena sativa L.) cultivars have been successfully transformed using an efficient and reproducible in vitro culture system for differentiation of multiple shoots from shoot apical meristems. The transformation was performed using microprojectile bombardment with two plasmids (pBY520 and pAct1-D) containing linked ( hva1-bar) and non-linked ( gus) genes. The hva1 and bar genes cointegrated with a frequency of 100% as expected, and 61.6% of the transgenic plants carried all three genes. Molecular and biochemical analyses in R0, R1 and R2 progenies confirmed stable integration and expression of all transgenes. Localization of the GUS protein in R0 and R1 plants revealed that high-expression of gus occurred in vascular tissues and in the pollen grains of mature flowers. The constitutive expression of HVA1 protein was observed at all developmental stages of transgenic plants, and was particularly stronger during the early seedling stages. R2 progeny of five independent transgenic lines was tested in vitro for tolerance to osmotic (salt and mannitol) stresses. As compared to non-transgenic control plants, transgenic plants maintained a higher growth and showed significantly ( P < 0.05) increased tolerance to stress conditions. Less than 10% of transgenic plants showed symptoms of wilting or death of leaves and, when these symptoms present were delayed in transgenic plants as compared to 80% of non-transgenic plants, either wilted or died. These symptoms confirmed the increased in vitro tolerance in hva1-expressing transgenic plants to non-transgenic plants, providing strong evidence that the HVA1 protein may play an important role in the protection of oats against salinity and possible water-deficiency stress conditions.

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“…The optimal medium for multiple shoot induction was MS medium supplemented with 2.22 µM 6-BA, which obtained 21.33 shoots per shoot apical meristem (Fig 1E), followed by MS medium supplemented with 2.22 µM 6-BA and 0.54 µM NAA (20.00). Phytohormones play an important role in multiple shoot induction [10]. The results showed effects of various combinations of 6-BA and NAA on the induction of multiple shoot clumps in lotus.…”

Section: The Effects Of Various Combinations Of 6-ba and Naa On Multimentioning

confidence: 95%

“…Until now, several studies have reported successful in vitro propagation of crops, such as maize, wheat, etc. [9,10], however, there are few reports describing in vitro culture of the lotus plant. Somatic embryogenesis from buds of lotus was reported by Arunyanart and Chaitrayagun [11], although the frequency was low.…”

Section: Introductionmentioning

confidence: 99%

In vitro plant regeneration of lotus (Nelumbo nucifera)

et al. 2015

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In this study, an efficient and reproducible plant regeneration system for lotus (Nelumbo nucifera Gaertn.) was established using shoot apical meristems from the buds and one-week-old aseptically germinated embryos as explants. Multiple shoot clumps were induced on Murashige and Skoog (MS) basal medium supplemented with various combinations of N6-Benzylaminopurine (6-BA) and α-Naphthalene acetic acid (NAA). The maximum response was obtained with 2.22 μM 6-BA, and produced 21.33 shoots per explant after four weeks. After five subcultures, multiple shoot clumps were transferred to MS basal medium supplemented with various combinations of 3-Indolebutyric acid (IBA), NAA and sucrose for root induction. After four weeks, plantlets with well-developed roots were achieved on MS basal medium supplemented with 0.54 μM NAA and 30 g/L sucrose with 100% rooting rate. The successfully acclimated plantlets were transferred to pots with the addition of 2 g/L KMnO4 into the soils, and finally fertile plants with much bigger leaves were obtained in the greenhouse. The survival rate was 97.33%.

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Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.)

Cited by 56 publications

References 22 publications

Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivumL.) Genotypes under Saline and Heat Stress Conditions

Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivumL.) Genotypes under Saline and Heat Stress Conditions

Competence of oat (Avena sativa L.) shoot apical meristems for integrative transformation, inherited expression, and osmotic tolerance of transgenic lines containing hva1

In vitro plant regeneration of lotus (Nelumbo nucifera)

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