Shoot multiplication of two &lt;em&gt;Sequoia sempervirens &lt;/em&gt; genotypes with addition of small concentrations of kinetin

Meneguzzi, Aline; Konzen, Enéas Ricardo; Navroski, Márcio Carlos; Camargo, Samila Silva; Pereira, Mariane de Oliveira; Rufato, Léo; Lovatel, Queli Cristina

doi:10.4336/2019.pfb.39e201701550

Cited by 2 publications

(4 citation statements)

References 17 publications

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“…In this study, the shoot induction, the number of shoots per explant, the root induction, and the number of roots per explant were affected by the explant's genotype. Our results are in concordance with the results reported by Meneguzzi et al 2 , who observed different genotypes' responses in shoot multiplication of S. sempervirens. Similarly, Sul and Korban 14 found differences in shoot proliferation and shoot elongation of S. sempervirens.…”

Section: Discussionsupporting

confidence: 94%

“…Endl. (Taxodiaceae) is a valuable forest species and occurs naturally in Western North America, especially in California 1,2 . This species is the tallest tree on earth with a high volume of standing biomass, in some stands exceeding 3500 metric tons/hectare 3 .…”

Section: Introductionmentioning

confidence: 99%

“…In the 21 st century, Korban and Soul 11 developed a procedure for the micropropagation of juvenile and adult material, and Lui et al 12 worked on the shoot regeneration and somatic embryogenesis from needles of redwood. The medium-term conservation of Sequoia sempervirens was investigated by Ozudogru et al 6 , and Meneguzzi et al 2 evaluated shoot multiplication of two Sequoia sempervirens genotypes with the addition of Kinetin.…”

Section: Introductionmentioning

confidence: 99%

“…1 Instituto de Investigación y Servicios Forestales, Universidad Nacional, Heredia, Costa Rica 2. NEIKER-BRTA, Department of Forestry Sciences, Arkaute.01192, Spain.Corresponding author: montalban@neiker.eus.…”

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Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Rojas-Vargas

Castander-Olarieta²,

Moncaleán³

et al. 2021

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Forest trees are renewable sources of timber and other valuable non-timber products. Nowadays, the increase in population and demand for forestry products results in overexploitation of forestry. Therefore, there is an urgent need to produce elite plants with higher productivity under stress derived from climate change to have available to afforestation. For this reason, propagation methods should be improved to be more efficient in terms of quality and productivity. The main species planted in the Basque Country is Pinus radiata; during the last three years, Pinus radiata plantations have suffered a fungus attack affecting mainly needles until the tree's death. This crisis is caused by the combined action of two fungi of the genus Dothistroma and Lecanosticta acicola, whose expansion seems to have been enhanced by weather-related factors, such as humid and hot summers. Although we have evidence of this disease's presence in our mountains since 1942, the disease has had a speedy expansion with an aggressive effect for reasons that are not scientifically known today. For the above, Basque Country forestry sector is looking for alternative species to be used in its plantations. Part of the forestry sector considers that Sequoia sempervirens could be a good choice for plantations. Besides, its high-quality wood and its tolerance to the attack of several pathogens and other diseases derived from climate change are characteristics that could confer some advantage over other forest species. The main goal of this study was to optimize the micropropagation of adult elite trees of Sequoia sempervirens. The effect of 6-benzylaminopurine, meta-Topolin and Kinetin, and 4 types of explant in the multiplication stage were analyzed to carry out this objective. Furthermore, the effect of two types of auxins: 1-naphthalene acetic acid, indole-3-butyric acid, and a mixture of both, were evaluated on the induction of roots and their subsequent effect on the acclimatization process. The best multiplication index was obtained when 4.4 µM 6-benzylaminopurine and apical explants longer than 1.5 cm of length were used. The root induction percentage was 75% in the most responsive genotype analyzed when 4.4 µM 6-benzylaminopurine was used on the induction stage, and 50 µM of 1-naphthalene acetic acid was used for rooting. Finally, after 3 months in the greenhouse, the explants cultured with Kinetin and rooted in a culture medium with indole-3-butyric acid showed the highest acclimatization success (94%).

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Rojas-Vargas

Castander-Olarieta²,

Moncaleán³

et al. 2021

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Controle da oxidação fenólica no cultivo in vitro de Astronium urundeuva (M. Allemão) Engl.

Silva

Oliveira

Nogueira

et al. 2023

Braz. J. Prod. Eng.

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Astronium urundeuva (aroeira do sertão) é uma das espécies nativas com potenciais silviculturais para exploração madeireira. No cultivo in vitro da espécie tem-se observado dificuldades como a oxidação fenólica, principalmente a partir de explantes coletados de árvores matrizes adultas. Dessa forma, a utilização de explantes originários de sementes é uma alternativa para a propagação da espécie, afim de minimizar a oxidação. Neste contexto, o objetivo do estudo foi avaliar a ação de ácido ascórbico, carvão ativado e polivinilpirrolidina no controle da oxidação fenólica no cultivo in vitro de A.urundeuva. Brotações apicais obtidas a partir de plântulas de A.urundeuva germinadas in vitro foram utilizadas como explantes. Estes foram subcultivados em meio de cultura MS50%, acrescido dos antioxidantes ácido ascórbico (0,2; 0,4; 0,6 e 0,8 mg.L-1) carvão ativado (3,0; 6,0; 9,0 e 12 g.L-1) e polivinilpirrolidona (0,5; 1,0; 1,5 e 2,0 g.L-1). Transcorridos 31 dias de cultivo in vitro, o ácido ascórbico, carvão ativado e PVP nas concentrações de 0,2 mg.L-1,12 g.L-1 e 1,5 g.L-1 respectivamente, foram efetivos no controle de oxidação fenólica. O carvão ativado nesta concentração controlou totalmente a oxidação fenólica das plântulas. Os resultados deste trabalho demonstram a viabilidade dos antioxidantes na minimização dos efeitos da oxidação fenólica, especialmente com o uso do carvão ativado e abre perspectivas para maiores estudos de micropropagação de A.urundeuva, tanto de materiais genéticos juvenis quanto adultos, contribuindo para a sua conservação e auxiliando em trabalhos de melhoramento genético da espécie.

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Shoot multiplication of two <em>Sequoia sempervirens </em> genotypes with addition of small concentrations of kinetin

Cited by 2 publications

References 17 publications

Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Controle da oxidação fenólica no cultivo in vitro de Astronium urundeuva (M. Allemão) Engl.

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