Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don

Abstract

The gesneriaceous perennial plant, Lysionotus serratus, has been used in traditional Chinese medicine. It also has a great development potential as an ornamental plant with its attractive foliage and beautiful flowers. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant was established in this study. High active cytokinin (6-benzyladenine (BA) or thidiazuron (TDZ)) was effective for direct organogenesis of initial induction. Murashige and Skoog (MS) growth media containing 0.5 mg L−1 BA alone or with combination of 0.1 mg L−1 α-Naphthaleneacetic acid (NAA) were the most effective for shoot proliferation. High BA concentration (1.0 mg L−1) in the media caused high percentage of vitrified shoots though they introduced high shoot proliferation rate. Histological observation indicated that adventitious shoot regeneration on the medium containing 0.5 mg L−1 BA alone occurred directly from leaf epidermal cells without callus formation. Regenerated shoots rooted well on medium containing half-strength MS medium with 0.5 mg L−1 indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA), and the plantlets successfully acclimatized and grew vigorously in the greenhouse with a 94.2% and 92.1% survival rate.

Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production

“…The shoot regeneration ability of R. carthamoides in terms of the percentage of explants producing organogenic calluses and number of buds/shoots per responding callus was tested by culturing segments of hypocotyl, cotyledons and roots on MS basal medium containing a combination of BA (0.2 or 0.5 mg L -1 ) with one of three auxins: 2,4-D, NAA or IBA (0.2 or 0.5 mg L -1 ; Table 1). The choice of media for induction of shoot-regenerating callus was based on our own preliminary experiments and a review of earlier studies (Li et al 2013;Zand et al 2014). Zand et al (2014) report the highest shoot regeneration (89 %) from R. carthamoides leaf explants cultured on MS medium supplemented with 2,4-D (0.25 mg L -1 ) and BA (1.5 mg L -1 ).…”

Micropropagation and assessment of genetic fidelity of Henckelia incana: an endemic and medicinal Gesneriad of South India

“…This indicates its high in vitro regeneration capacity. The development of shoots directly from the surface of leaves without callus formation has also been reported in a number of Gesneriaceae plants (Vazquez et al 1977;Wuttisit and Kanchanapoom 1996;Ma et al 2011;Li et al 2013).…”

“…Among the members of Gesnericaeae, efficient regeneration protocols have been established in Achimenes (Vlahos et al 1995), Saintpaulia (Lo 1997), Didymocarpus (Krishnarajah et al 2002;Shaib et al 2004), Kholeria (Kozak et al 2007), Chirita (Tan et al 2009), Aeschynanthus (Cui et al 2009), Ramonda (Dontcheva et al 2009), Streptocarpus (Wei et al 2010), Metabriggsia (Ma et al 2011), Titanotrichum (Takagi et al 2011), Sinningia (Park et al 2012), Primulina (Qin et al 2013) and Lysionotus (Li et al 2013). The efficient micropropagation system calls for the genetic stability of in vitro regenerated plantlets.…”

Ex situ preservation for some endemic and rare medicinal plants in Taif, KSA

“…Effect of high concentration of BA on shoot vitrification was studied earlier in different reports [40]. High percentage of vitrified shoots was observed on medium containing 1.0 mg/L BA [41]. Vitrification could be represented by poorly developed vascular bundles, abnormal functioning and stomata abnormal wax quality [42] and it is the consequence of culture conditions [43].…”