Shoot regeneration from nodules of Charybdis sp.: A comparison of semisolid, liquid and temporary immersion culture systems

Wawrosch, Christoph; Kongbangkerd, Anupan; Köpf, Andreas; Kopp, Brigitte

doi:10.1007/1-4020-3200-5_19

Cited by 9 publications

(6 citation statements)

References 8 publications

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“…TIS has resulted in an important improvement in the micropropagation of potatoes (Solanum tuberosum L.), banana (Musa x paradisiaca L.) and yucca as reported by Etienne and Berthouly (2002), but results with bulbous plants have been moderately better or not different when compared to conventional tissue culture systems (e.g., Charybdis sp. and Hippeastrum x chmielii) (Wawrosch et al, 2005;Ruffoni and Savona, 2005;Ilczuk et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species

Muñoz

Seemann

Riegel

2009

Chilean J. Agric. Res.

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Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are not well-known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared liquid and solid media, and in the third experiment, a temporary immersion system (TIS), and conventional culture in static liquid, shaken liquid and gelled Murashige and Skoog (MS) media were compared. By using larger (350 mL) flasks with higher (50 mL) media volume, 100% more fresh weight of microbulb was obtained that treatment with smaller flasks (45 mL) and media volume (10 mL). In gelled medium, hyperhydricity affected only 5% of explants, while in liquid medium was 16-40%. Survival to acclimatization reached 87-94% for plants from gelled medium; from liquid medium only 38-69%. TIS yielded higher propagation rate (1.9 shoots in 30 d) compared with shaken liquid medium (1.0) (P < 0.05) in R. ananuca only. Current procedures are appropriate for the support of ex situ conservation and germplasm bank establishment.

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Section: Resultsmentioning

confidence: 99%

Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species

Muñoz

Seemann

Riegel

2009

Chilean J. Agric. Res.

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“…Shoots regeneration of Charybdis sp. were optimal in immersion frequency 5 min each 24 h [47]. Immersion interval 3 h caused explants contact with medium more frequent, so explants were lack of oxygen, although length of immersion only 5 min.…”

Section: Resultsmentioning

confidence: 99%

Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system

Pramita

Kristanti

Sugiharto

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Gynura procumbens (Lour.) Merris one of medicinal plant which was carried out used as antioxidant, anticancer, anti-inflammatory, hepatoprotective, and antimicrobial. Many strategies were used to increase the production of biomass and valuable compounds. This study was to investigate the variation effect of growth regulators and immersion frequency on production of biomass and flavonoid contained of G. procumbens shoots culture in temporary immersion bioreactor. Stem nodes were used as an explants and induction of shoots were done in solid MS medium supplemented with many kinds of growth regulator. The best treatments were used to produce biomass and flavonoid compounds in temporary immersion bioreactor; there are combination of IAA 2 mg/L and BA 4, 6, 8 mg/L and immersion frequency (5 min each 3 h; 15 min each 12 h). Results showed that the growths of G. procumbens shoots in solid MS medium were influenced by supplementation of growth regulators. MS medium supplemented with single cytokinine (6 mg/L kinetin) and combination of auxin (IAA) and cytokinine (BA) caused increasing of shoots growth. Production of biomass of G. procumbens in temporary immersion bioreactor was achieved in long immersion interval (12 h) and highest flavonoid production was obtained in combination treatment of immersion frequency 15 min each 12 h and MS medium supplemented with IAA 2 mg/L, BA 8 mg/L.

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“…However, micropropagation can be a labor intensive and time‐consuming task depending on the nature of the species. Semi‐solid medium is commonly used for micropropagation to provide support for the developing plants even though liquid culture has been shown to provide better growth for many species can reduce subculturing by allowing media to be replenished, and is more compatible with automation. Another problem associated with micropropagation using semi‐solid media during the rooting phase is that the gel must be manually washed from the delicate roots to prevent microbial growth in the greenhouse/growth facility.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, the use of liquid media is generally more productive and leads to higher multiplication rates compared to conventional gel based methods of micropropagation. Comparative studies have shown that liquid is better than semi‐solid medium for a number of species . Rooting in a liquid medium is particularly advantageous and cost effective because gel does not need to be carefully washed from the roots.…”

Section: Introductionmentioning

confidence: 99%

Improved in vitro rooting in liquid culture using a two piece scaffold system

Shukla

Piunno

Saxena

et al. 2019

Engineering in Life Sciences

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Plant tissue culture techniques have been used to propagate horticultural crops at a commercial scale for more than three decades. However, due to the high cost it is generally only used for high value crops. To increase production efficiency and make micropropagation viable for a wider range of species, new approaches to address key steps of the process with high labor inputs need to be evaluated. For this study, a two‐piece scaffold system was designed, prototyped using 3D printing, and tested to physically hold plants upright thereby facilitating liquid based rooting. This system was evaluated with Malus domestica, Betula lenta, and Musa sp. using static liquid culture as well as rocker based temporary immersion system and compared to rooting in semi‐solid based medium as is commonly practiced. Significantly, earlier rooting was observed in all three species in liquid when compared to semi‐solid culture system, and plants cultured in liquid on the rocker generally performed better than those in static liquid. In addition to quicker, more uniform rooting, reducing labor requirements, and preventing root damage. This newly designed system is simple, easy to use, will help to improve efficiency, and reduce the cost of micropropagation.

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Shoot regeneration from nodules of Charybdis sp.: A comparison of semisolid, liquid and temporary immersion culture systems

Cited by 9 publications

References 8 publications

Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species

Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species

Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system

Improved in vitro rooting in liquid culture using a two piece scaffold system

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