Shoot tip culture and thermotherapy for recovering virus-free plants of garlic

Fajardo, T. V. M.; Dusi, A. N.; Resende, R. de O.; Buso, J. A.

doi:10.1590/s0102-05362000000300010

Cited by 24 publications

(17 citation statements)

References 5 publications

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“…Garlic shoot tip culture associated with dry heat treatment (cloves exposed to 37°C for 35 days) were essential for recovering virus-free plants of the Amarante cultivar. In this condition, 70% of the explants developed in vitro and produced plants (Torres et al, 2000). Meristem culture of potato using explants 100 μm in length combined with thermotherapy at approximately 37±1°C for 40 days produced a high rate of virus-free plants, 81% in the Binella cultivar and 75% in the Burren cultivar (Almaarii et al, 2012).…”

Section: Figurementioning

confidence: 99%

Elimination of shallot bulb viruses through heat treatment

2017

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Shallot (Allium cepa L. Aggregatum group) is usually cultivated vegetatively. As a result, viruses tend to accumulate within the host plants and spread to healthy plants every crop cycle, reducing yield and bulb quality. There are a very limited number of studies about the elimination of shallot viruses through heat treatment. The objective of this research was to eliminate shallot viruses through heat treatment to produce virus-free plantlets.The leaves of Biru Lancor with specific visual virus symptoms were detected by Reverse TranscriptionPolymerase Chain Reaction (RT-PCR). Then bulbs of Biru Lancor that were positively infected by viruses were used as materials for heat treatment. The treatments were a control (without treatment), electric treatment at 15 mA for 10 minutes, heat treatment in an incubator at 37°C for 4 weeks, heat treatment in a waterbath at 45°C for 60 minutes, and combination of heat treatment in an incubator at 37°C for 4 weeks and heat treatment in a waterbath at 45°C for 60 minutes. After being subjected to heat treatment, the pseudo stem were cultivated in the MS Medium + 1 mg/L BAP + 1 mg/L IBA.Virus detection by RT-PCR was conducted 28 days after planting using samples of leaves from each plantlet.The results of this research showed that the treatments of electric treatment at 15 mA for 10 minutes and combination of heat treatment in the incubator at 37°C for 4 weeks and heat treatment in the waterbath at 45°C for 60 minutes could suppress the incidence of Shallot latent virus (SLV) until 100%. Heat treatment might have an important role in the degradation of virus particles by boosting Virus-Induced Gene Silencing (VIGS) as plant responses to virus infection.

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Section: Figurementioning

confidence: 99%

Elimination of shallot bulb viruses through heat treatment

2017

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“…Meristem tissues that had undergone thermotherapy resulted in regeneration of fewer plants than the nontreated meristems, but 90 to 100% plants from thermotherapy treated meristem tissues were found to be free from OYDV. Torres et al, (2000) recovered 90% plants free of virus from the cloves of garlic cultivar Amarante through dry heat thermotherapy (37 °C for 35 days). However, according to Ramirez et al, (2006) though mericlones produced through thermotherapy were found to be free from viruses, but the overall efficiency of virus elimination was low.…”

Section: Thermotherapymentioning

confidence: 99%

Validation of RNA Interference for Improvement in Mustard (Brassica sp.): A Review

Boken¹,

Singh²,

Kaur³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Jenis virus tertentu juga sulit dieliminasi sehingga memerlukan kombinasi perlakuan tertentu (Wang & Valkonen 2012). Torres et al (2000) melaporkan bahwa perlakuan termoterapi atau kultur meristem yang diaplikasikan secara tunggal tidak dapat mengeliminasi virus pada bawang putih, namun ketika kedua perlakuan tersebut dikombinasikan maka eliminasi virus dapat terjadi. Eliminasi virus juga terjadi pada kentang yang diberi kombinasi perlakuan termoterapi dan kultur meristem (Ali et al 2013).…”

Section: Pendahuluanunclassified

Uji Efikasi Teknik Kultur Meristem dan Kemoterapi untuk Eliminasi Sugarcane Streak Mosaic Virus (SCSMV) pada Tebu Efficacy of Meristem Culture and Chemotherapy for Elimination of Sugarcane

Roostika

Harsono

Efendi

et al. 2017

Bul. Tan. Tembakau, Serat & Minyak Industri

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ABSTRAKPenggunaan benih bebas virus merupakan salah satu cara pengendalian penyakit virus. Jaringan tanaman dapat dibebaskan dari virus melalui aplikasi teknik eliminasi virus, seperti termoterapi, kemoterapi, kultur meristem, dan krioterapi. Tujuan penelitian ini adalah untuk menguji respon varietas tebu terhadap perlakuan teknik kultur meristem dan kemoterapi dengan bahan antiviral, serta untuk mengetahui efektivitasnya dalam mengeliminasi virus sugarcane streak mosaic virus (SCSMV) pada tebu. Penelitian ini dilakukan pada April−November 2015 di Laboratorium Kultur Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian dan Laboratorium Virologi, Fakultas Pertanian, Universitas Gadjah Mada. Penelitian terdiri atas tiga tahap, yaitu 1) Deteksi virus dari tanaman induk, 2) Aplikasi teknik kultur meristem dan kemoterapi, serta 3) Indeksing virus. Bahan tanaman yang digunakan adalah sebelas varietas tebu (GMP3, PS865, dan Kentung asal Bogor, PS862 dan Cening asal Cirebon, PS881 asal Jember, PSJK922 asal Malang, serta PS864, PS881, PSJK922, PSJT941 asal Pati). Deteksi virus dilakukan secara RT-PCR dengan primer universal MJ dan primer spesifik SCSMV. Bahan antiviral yang digunakan untuk kemoterapi adalah Ribavirin (0 dan 25 µg/l). Hasil uji RT-PCR menggunakan primer universal MJ menunjukkan bahwa empat varietas (GMP3 asal Bogor, PS864 dan PSJT941 asal Pati, serta Cening asal Cirebon) terinfeksi oleh Potyvirus. Empat varietas lainnya (PS862 asal Cirebon, PS881 asal Jember, PSJK922 asal Malang, dan Kentung asal Bogor) terbukti terserang virus SCSMV berdasarkan uji RT-PCR dengan primer spesifik. Seluruh meristem mampu beregenerasi membentuk tunas. Penggunaan Ribavirin 25 µg/l tidak menyebabkan penurunan daya tumbuh meristem (50−100%), bahkan seluruh varietas mampu bermultiplikasi tunasnya dibandingkan dengan kontrol yang hanya memiliki daya tumbuh 0−100%, dan tidak semua varietas mampu bermultiplikasi tunasnya. Secara tunggal, aplikasi teknik kultur meristem tidak mampu mengeliminasi virus SCSMV, namun jika dikombinasikan dengan perlakuan kemoterapi maka virus SCSMV dapat tereliminasi dengan efikasi sebesar 44,4%.Kata kunci: Tebu, Ribavirin, Potyvirus, SCSMV, RT-PCR ABSTRACTThe use of virus-free seedling is an option for controlling viral disease that can be obtained through the application of viral elimination method. Plant tissues can be eliminated from virus infection by applying virus thermotherapy, chemotherapy, meristem culture, and cryotherapy. The research objectives were to examine the response of sugarcane varieties to meristem culture treatments and antiviral agent and also to determine the efficacy rate of both techniques in eliminating SCSMV disease. The study was conducted at Tissue Culture Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Develpoment, and also at Virology Laboratory, Faculty of Agriculture, Gadjah Mada University. This study consisted of three activities: 1) Virus detection of the mother plants, 2) App...

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Shoot tip culture and thermotherapy for recovering virus-free plants of garlic

Cited by 24 publications

References 5 publications

Elimination of shallot bulb viruses through heat treatment

Elimination of shallot bulb viruses through heat treatment

Validation of RNA Interference for Improvement in Mustard (Brassica sp.): A Review

Uji Efikasi Teknik Kultur Meristem dan Kemoterapi untuk Eliminasi Sugarcane Streak Mosaic Virus (SCSMV) pada Tebu Efficacy of Meristem Culture and Chemotherapy for Elimination of Sugarcane

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