Introduction: There has been limited data on sleep disturbances in Singapore, a multi-ethnic Asian population. We aimed to quantify the prevalence of poor sleep quality and examine associations with sociodemographics, lifestyle risk factors and medical comorbidities. Methods: The Singapore Health 2012 (SH2012) and Singapore Health 2 (SH2) were two population-based studies that comprised interviews by trained personnel on sociodemographics, lifestyle factors and medical history together with health screening for comorbidities such as hypertension and diabetes. A community sample of 4666 people aged 18 to 80 years old from these two cohorts completed the Pittsburgh Sleep Quality Index (PSQI). Results: The mean age of the sample was 44.7 AE 15.0 years and 2162 (46.2%) were male. The prevalence of poor sleep quality, defined as a PSQI score > 5, was 27.2%. There was no association between gender and sleep quality. Participants who were of Malay ethnicity, elderly (aged ≥65 years), obese (BMI ≥27.5 kg/m2), previously married, those with lower household incomes and levels of education, smokers and medical comorbidities were more likely to report poor sleep quality (p-value all < 0.01) On logistic regression analysis, the independent predictors of poor sleep quality were chronic joint pain (odds ratio [OR] 1.96, 95% Confidence Interval [CI]: 1.66-2.31), cancer (OR 1.76, 95% CI: 1.12-2.78), cardiovascular disease (OR 1.63, 95% CI: 1.22-2.19), having a monthly household income <$2,000 (OR 1.46 95% CI 1.08-1.54), smoking (OR 1.46, 95% CI: 1.15-1.85) and being of Malay ethnicity (OR 1.28 95% CI: 1.07-1.53). Discussion: Poor sleep quality is prevalent among Singaporean adults and is associated with sociodemographic factors and presence of medical comorbidities. Considering the impact of poor sleep quality on health, our findings suggest a potentially large burden of health consequences caused by sleep disturbances in the general population.