Short- and long-wavelength-sensitive opsins are involved in photoreception both in the retina and throughout the central nervous system of crayfish

Kingston, Alexandra C. N.; Cronin, Thomas W.

doi:10.1007/s00359-015-1043-2

Cited by 26 publications

(36 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study provides robust data to demonstrate the different responses in the CPR neurons located in the left and right side of the last ganglion of an isolated nerve cord including the intensities of light, colors of light, and temperature, by extracellular recordings of action potentials. Our results support the presence of the SWS, and the LWS opsins in the CPRs, which showed sensitivities to blue and green light, respectively [20,21]. In addition, the current results provide support for the functional asymmetries described recently as a novel property for both CPR-L and -R when comparing their responses induced by white light pulses in the same species of crayfish C. quadricarinatus [34].…”

Section: Discussionsupporting

confidence: 88%

“…Other results have illustrated light-induced activity from the CPR by using a cold white light lamp (light emitting diode), which emitted light with two peaks: blue (446 nm) and green (557 nm) [19]. Kingston and Cronin [20] identified two visual opsin proteins in the crayfish P. clarkii. The short-wavelength-sensitive (SWS) opsin from blue light (440 nm), and the long-wavelength-sensitive (LWS) opsin from green light (530 nm).…”

Section: Introductionmentioning

confidence: 99%

“…The short-wavelength-sensitive (SWS) opsin from blue light (440 nm), and the long-wavelength-sensitive (LWS) opsin from green light (530 nm). Both opsins are located in the sixth abdominal ganglion, and hence it has been postulated that these opsins participate in photoreception from the CPR [20,21]. The absorption properties of the light wavelength of the photopigments depend on their opsins [22,23].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Asymmetric Firing Rate from Crayfish Left and Right Caudal Photoreceptors Due to Blue and Green Monochromatic Light Pulses

et al. 2018

View full text Add to dashboard Cite

Recent studies have postulated that the left and right caudal photoreceptors (CPR-L and CPR-R, respectively) of the crayfish show asymmetry of spontaneous activity in darkness and responses induced by white light. Two photopigments have been identified; the first one sensitive to blue light and the second one sensitive to green light. This study explores blue and green monochromatic light responsiveness with respect to both CPR-L and -R, as well as the effects of temperature on these photoreceptors. We performed simultaneous extracellular recordings of the firing rate of action potentials from CPRs of the crayfish Cherax quadricarinatus (n = 12). At room temperature (24 ± 1 °C), CPR-L and -R showed a significant difference in the spikes from most of the comparations. CPRs in the dark exhibited spontaneous asymmetric activity and displayed sensitivity to both monochromatic light sources. CPR responses were light intensity dependent within a range of 1.4 logarithmic intensity units, showing approximately 0.5 logarithmic intensity units more sensitivity to blue than to green light. The CPRs displayed an asymmetrical response to both colors by using a constant light intensity. At 14 (±1) °C, activity in darkness diminished while asymmetry persisted, and the CPRs improved responses for both monochromatic light sources, displaying a significant asymmetry. Here, we provide additional evidence of the asymmetric activity in darkness and light response from the CPRs. The new data allow further investigations regarding the physiological role of caudal photoreceptors in the crayfish.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Asymmetric Firing Rate from Crayfish Left and Right Caudal Photoreceptors Due to Blue and Green Monochromatic Light Pulses

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Xenopus tadpoles (Currie et al, 2016), and invertebrates such as insects (Hariyama, 2000), and crustaceans decapods (Simon and Edwards, 1990;Bobkova et al, 2003;Kingston and Cronin, 2015) and the amphipod Talitrus (Frelon-Raimond et al, 2005). The present study also demonstrated that short-wavelength light is preferentially used by the non-ocular photosensory system (Figs 2,3 and 6).…”

Section: ) Andsupporting

confidence: 73%

Light avoidance by non-ocular photosensing system in the terrestrial slugLimax valentianus

Nishiyama

Nagata

Matsuo

et al. 2019

Journal of Experimental Biology

View full text Add to dashboard Cite

Although the eye is the best-studied photoreceptive organ in animals, the presence of non-ocular photosensing systems has been reported in numerous animal species. However, most of the roles that nonocular photosensory systems play remain elusive. We found that the terrestrial slug Limax valentianus avoids light and escapes into dark areas even if it is blinded by the removal of the bilateral superior tentacle. The escape behaviour was more evident for short-wavelength light. Illumination to the head with blue but not red light elicited avoidance behaviour in the blinded slugs. Illumination to the tail was ineffective. The light-avoidance behaviour of the blinded slugs was not affected by the removal of the penis, which lies on the brain in the head, suggesting that the penis is dispensable for sensing light in the blinded slug. mRNA of Opn5A, xenopsin, retinochrome and, to a lesser extent, rhodopsin was expressed in the brain according to RT-PCR. Lightevoked neural responses were recorded from the left cerebro-pleural connective of the isolated suboesophageal ganglia of the brain, revealing that the brain is sensitive to short wavelengths of light (400-480 nm). This result is largely consistent with the wavelength dependency of the light-avoidance behaviour of the blinded slugs that we observed in the present study. Our results strongly support that the terrestrial slug L. valentianus detects and avoids light by using its brain as a light-sensing organ in the absence of eyes.

show abstract

“…The crayfish Procambarus clarkii has a well-characterized extraocular photoreceptor, called the caudal photoreceptor, located in the sixth abdominal ganglion. SWS and LWS opsin transcripts have been observed not only in the sixth abdominal ganglion, but also in all ganglia of the nerve cord (Kingston and Cronin, 2015). The functional role of the caudal photoreceptor is extended to the triggering of the tail reflex, and to the walking behaviour of the animal.…”

Section: Discussionmentioning

confidence: 97%

The opsin repertoire of the Antarctic krill Euphausia superba

et al. 2016

View full text Add to dashboard Cite

The Antarctic krill Euphausia superba experiences almost all marine photic environments throughout its life cycle. Antarctic krill eggs hatch in the aphotic zone up to 1000 m depth and larvae develop on their way to the ocean surface (development ascent) and are exposed to different quality (wavelength) and quantity (irradiance) of light. Adults show a daily vertical migration pattern, moving downward during the day and upward during the night within the top 200 m of the water column. Seawater acts as a potent chromatic filter and animals have evolved different opsin photopigments to perceive photons of specific wavelengths. We have investigated the transcriptome of E. superba and, using a candidate gene approach, we identified six novel opsins. Five are r-type visual opsins: four middle-wavelength-sensitive (EsRh2, EsRh3, EsRh4 and EsRh5) and one long-wavelengthsensitive (EsRh6). Moreover, we have identified a non-visual opsin, the EsPeropsin. All these newly identified opsin genes were significantly expressed in compound eyes and brain, while only EsPeropsin and EsRh2 were clearly detected also in the abdomen. A temporal modulation in the transcription of these novel opsins was found, but statistically significant oscillations were only observed in EsRrh3 and EsPeropsin. Our results contribute to the dissection of the complex photoreception system of E. superba, which enables this species to respond to the daily and seasonal changes in irradiance and spectral composition in the Southern Ocean.

show abstract

Short- and long-wavelength-sensitive opsins are involved in photoreception both in the retina and throughout the central nervous system of crayfish

Cited by 26 publications

References 14 publications

Asymmetric Firing Rate from Crayfish Left and Right Caudal Photoreceptors Due to Blue and Green Monochromatic Light Pulses

Asymmetric Firing Rate from Crayfish Left and Right Caudal Photoreceptors Due to Blue and Green Monochromatic Light Pulses

Light avoidance by non-ocular photosensing system in the terrestrial slugLimax valentianus

The opsin repertoire of the Antarctic krill Euphausia superba

Contact Info

Product

Resources

About