Short bowel syndrome: Experimental approach to increase intestinal surface in rats by gastric homologous acellular matrix

Parnigotto, Pier Paolo; Marzaro, Maurizio; Artusi, T; Perrino, G; Conconi, Maria Teresa

doi:10.1053/jpsu.2000.9309

Cited by 35 publications

(27 citation statements)

References 15 publications

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“…Moreover, the basal membrane, which plays an important role in keeping selective permeability and tissue integrity, was also preserved (5). The DEM approach has been proven as an effective method to obtain ECM-derived prosthetic grafts, which can support in vitro and in vivo cell adhesion and proliferation: acellular matrices are remodelled in living tissues and can function as urethra (13), small bowel (14), skeletal muscle (15), esophagus (16) and tracheal (17) substitutes. However, while many studies focused on the decellularized tissue biocompatibility, cell seeding and implantation potential, only few reported findings on relationships between tissue decellularization and the physical and mechanical properties of tissue (7,18).…”

Section: Decellularized Bovine Reinforced Vessels For Small-diameter mentioning

confidence: 99%

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Grandi

Baiguera²,

Martorina³

et al. 2011

Int J Mol Med

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Abstract. The aim of the present study was to investigate the influence of a decellularization protocol on the structure and the mechanical behavior of small-diameter (<6 mm) tibial calf arteries and veins. Calf vessels were decellularized by a detergent-enzymatic method (DEM), partially hydrolyzed with trypsin and subsequently cross-linked using poly(ethylene glycol) diglycidyl ether. Our results showed that i) the DEM can be considered a simple and valuable procedure for the preparation of complete acellular arteries and veins able to preserve a high degree of collagen and elastic fibers, and ii) poly(ethylene glycol) diglycidyl ether cross-linking treatment provides appropriate mechanical reinforcement of blood vessels. Histologically, the decellularized vessels were obtained employing the detergent-enzymatic procedure and their native extracellular matrix histoarchitecture and components remained well preserved. Moreover, the decellularization protocol can be considered an effective method to remove HLA class I antigen expression from small-diameter tibial calf arteries and veins. Cytocompatibility of decellularized cross-linked vessels was evaluated by endothelial and smooth muscle cell seeding on luminal and adventitial vessel surfaces, respectively.

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Section: Decellularized Bovine Reinforced Vessels For Small-diameter mentioning

confidence: 99%

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Grandi

Baiguera²,

Martorina³

et al. 2011

Int J Mol Med

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“…Moreover, studies on ß-cells cultured on various crude matrices or on their purified extracts highlighted the (17,(21)(22)(23)(24)(25)(26). Tissue engineering studies have also shown that scaffolds derived from decellularized matrices, obtained after the removal of the cellular component, support in vitro adhesion, growth, and function of several cell types (27)(28)(29) and act as a template for growth and tissue remodelling (30)(31)(32)(33)(34)(35). Based on these considerations, in this study we ascertained whether pancreas and/or liver acellular matrices support the pancreatic islet secretory function.…”

Section: Introductionmentioning

confidence: 99%

Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

Carlo

Baiguera²,

Conconi³

et al. 2009

Int J Mol Med

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Abstract. Increasing pancreatic islet survival and function is a starting point for obtaining a valuable bioartificial pancreas for the treatment of type 1 diabetes. In this context, decellularized matrices, obtained after the removal of tissue cellular part, are known to support in vitro adhesion, growth, and function of several cell types. We demonstrate that a homologous acellular pancreatic matrix is a suitable scaffold for rat islet cultures maintaining their long-term viability and function. Islets adhered to the pancreatic matrix showed a constant glucose-induced insulin release during long-term in vitro incubation, while islets cultured without a matrix or on the liver matrix showed a progressive reduction. In order to obtain implantable devices, acellular matrix/islet cultures were entrapped into poly(vinyl alcohol) (PVA)/ poly(ethylene glycol) (PEG) tubes obtained by the freezing/thawing procedure. Under this condition, an in vitro constant insulin release was detected. The devices were then implanted into diabetic rats where reduced insulin requirement was noted suggesting insulin secretory activity of islets contained in the device. Indeed, immunofluorescence confirmed the presence of insulin-and glucagon-producing cells into the explanted devices. These data show that PVA/PEG semi-permeable membrane can obtain devices that restore, at least in part, insulin secretion.

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“…Therefore a critical step in skeletal muscle-tissue engineering is the identification of the optimal biomaterial scaffold, able to promote normal differentiation and maturation of myoblasts into myotubes and myofibers. At present, several kinds of natural scaffolds, such as porcine small intestinal submucosa (Badylak et al, 2002), acellular dermal matrix (Chung et al, 2003) and collagen (Lai et al, 2003) (Roeder et al, 1999) and could be employed as promising tissue substitutes (Parnigotto et al, 2000a(Parnigotto et al, , 2000bSutherland et al, 1996).…”

Section: Morphological Evaluation Of the Myoblast Seeded Scaffolds Inmentioning

confidence: 99%

Tissue Engineering for Tissue and Organ Regeneration

Eberli¹

2011

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Short bowel syndrome: Experimental approach to increase intestinal surface in rats by gastric homologous acellular matrix

Cited by 35 publications

References 15 publications

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

Tissue Engineering for Tissue and Organ Regeneration

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