2013
DOI: 10.1111/tpj.12379
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Short‐chain dehydrogenase/reductase catalyzing the final step of noscapine biosynthesis is localized to laticifers in opium poppy

Abstract: SUMMARYThe final step in the biosynthesis of the phthalideisoquinoline alkaloid noscapine involves a purported dehydrogenation of the narcotinehemiacetal keto moiety. A short-chain dehydrogenase/reductase (SDR), designated noscapine synthase (NOS), that catalyzes dehydrogenation of narcotinehemiacetal to noscapine was identified in opium poppy and functionally characterized. The NOS gene was isolated using an integrated transcript and metabolite profiling strategy and subsequently expressed in Escherichia coli… Show more

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Cited by 43 publications
(43 citation statements)
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“…The same approach was used to isolate scoulerine 9-O-methyltransferase (SOMT1), canadine synthase (CYP719A21), and noscapine synthase (NOS) (Fig. 1), along with two other O-methyltransferases (SOMT2 and SOMT3) (5,6,11). CYP82Y1, CYP82X1, CYP82X2, and CYP719A21 transcripts were detected in 454 pyrosequencing databases of opium poppy chemotypes accumulating noscapine or narcotoline (Fig.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The same approach was used to isolate scoulerine 9-O-methyltransferase (SOMT1), canadine synthase (CYP719A21), and noscapine synthase (NOS) (Fig. 1), along with two other O-methyltransferases (SOMT2 and SOMT3) (5,6,11). CYP82Y1, CYP82X1, CYP82X2, and CYP719A21 transcripts were detected in 454 pyrosequencing databases of opium poppy chemotypes accumulating noscapine or narcotoline (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…The final step was validated by the recent isolation and characterization of noscapine synthase (NOS), a short chain dehydrogenase/reductase that converts narcotinehemiacetal to noscapine in opium poppy (see Fig. 1) (11). O-Methylation of a secoberbine intermediate at the position equivalent to the C4Ј hydroxyl group of noscapine was attributed to one of three O-methyltransferases in the gene cluster.…”
mentioning
confidence: 99%
“…The function of metabolic gene clusters in plants has been suggested as a mechanism to regulate expression at the epigenetic level (wegel et al 2009) and to facilitate complete inheritance of a specialized metabolic pathway (Swaminathan et al 2009). Recently, CAS (Dang and Facchini 2014a), N-methylcanadine 1-hydroxylase (CYP82Y1) (Dang and Facchini 2014b) and noscapine synthase (NOS) (Chen and Facchini 2014) were isolated and characterized from opium poppy. NOS was characterized as a NAD + -dependent, short-chain dehydrogenase/ reductase catalyzing the irreversible conversion of narcotinehemiacetal to noscapine.…”
Section: Noscapinementioning
confidence: 99%
“…Recently, the application of shotgun proteomics to determine the relative abundance of proteins in isolated latex, or in whole stem including latex, revealed that the final three steps in morphine biosynthesis, catalyzed by T6ODM, COR and CODM, occur primarily in laticifers (Onoyovwe et al 2013). Other BIA biosynthetic enzymes abundant in laticifers include NOS (Chen and Facchini 2014) and 7OMT (Onoyovwe et al 2013), indicating that the final steps in the formation of noscapine and 7-O-methylated derivatives of reticuline also occur in latex and are spatially separated from upstream enzymes. Top candidates for transport from sieve elements to laticifers include thebaine and narcotinehemiacetal in the morphine and noscapine pathways, respectively.…”
Section: Cellular Localization and Transportmentioning
confidence: 99%
“…The presence or absence of transcripts corresponding to clustered genes is also observed. Tough, more information is needed, existing evidence suggests coordinated regulation (Chen and Facchini 2014). Suppression of a gene involved in noscapine biosynthesis pathway (CYP82Y1) resulted in reduction of noscapin, and changes in expression level of noscapine producing genes.…”
Section: Discussionmentioning
confidence: 99%