Short-Circuit Current Across Isolated Rabbit Ileum, Jejunum and Duodenum

Glew, Richard H.; Moore, Edward; Wallace, Craig K.

doi:10.3181/00379727-142-37132

Cited by 1 publication

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“…Rapid changes in PD, SCC, and R, similar to those described in rabbit ileum (Glew et al, 1973), occurred with both jejunal and ileal mucosa on mounting in the flux chambers. After a rapid initial rise, a fall in PD was seen in nearly all tissues followed by a second rise of variable magnitude.…”

Section: Electrical Measurementssupporting

confidence: 65%

Human intestinal ion transport in vitro.

Corbett¹,

Isaacs²,

Riley³

et al. 1977

Gut

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sUMMARY The transport of sodium and chloride across human jejunal and ileal mucosa was studied using an in vitro technique. Specimens of mucosa removed at operation were stripped of muscle coats, mounted in specially designed Perspex flux chambers and bathed in warmed oxygenated and stirred buffer solutions.Evidence was obtained for the active transport of sodium in both jejunum and ileum and of chloride in the ileum. Sodium absorption was enhanced by glucose in both regions of the gut but net chloride transport was unaffected. Glucose had a greater effect on sodium transport in the ileum than the jejunum.The electrical potential difference and resistance was greater and undirectional ion fluxes smaller in jejunal than ileal mucosa.Many of these results with human intestine are similar to results reported with in vitro animal intestine. Apparent discrepancies between the behaviour in vivo of human intestine and in vitro of animal intestine are thus likely to be due predominantly to technical rather than species differences.Much current information about mechanisms of intestinal electrolyte transport rests on the results of in vitro investigations using animal intestine.Human intestinal ion transport on the other hand has been studied mainly by in vivo techniques and relatively few in vitro investigations have been undertaken. Such discrepant observations as the failure of glucose to enhance sodium absorption in vivo in human ileum (Fordtran et al., 1968) but not in vitro in the rabbit ileum (Schultz and Zalusky, 1964b) and the failure to demonstrate bicarbonate absorption in vitro in rabbit jejunum (Fromm, 1973), while in vivo in man it clearly occurs (Fordtran et al., 1968), could be due to species differences or to technical variations. The present human in vitro studies were undertaken for comparison with in vitro animal and in vivo human intestinal behaviour. Methods

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Section: Electrical Measurementssupporting

confidence: 65%