Short Communication

Abstract

Although nowadays most oocyte retrievals for in-vitro fertilization (IVF) are performed using a transvaginal, ultrasound-directed technique, monitoring of follicular development is still often performed via the abdominal route. On the bases of 106 sonographies carried out on 71 patients during ovulation monitoring within an IVF programme, we demonstrated that the number of follicles visualized endovaginally was significantly higher than that visualized by the abdominal route: n = 1124 versus n = 772 respectively, and that their size was significantly greater using the transvaginal approach: 20.8 mm versus 18.8 mm respectively, for the largest follicle. Consequently, if ovulation monitoring is carried out transvaginally, the follicular size criteria on which human chorionic gonadotrophin is administered must be reconsidered.

A new possibility in fertility preservation: The artificial ovary

“…Offspring through natural mating (Carroll & Gosden, 1993) 2008 (Silber et al, 2005;Silber et al, 2008;Silber & Gosden, 2007) (J. Donnez et al, 2004;Meirow et al, 2005;Povoa et al, 2016;Revel et al, 2011) -Allart et al, 1991;Lass et al, 1997;Schmidt et al, 2005;Wenners et al, 2017) Cryopreservation efficiency Florescent staining for viability and morphological evaluation under microscope (J. Donnez et al, 2004;Martinez-Madrid et al, 2004) After transplantation Oxygenation Electron paramagnetic resonance, PET/CT, NIRS, HSI usage, intravital microscopy (Dejust et al, 2017;Fisher et al, 2016;Holmer et al, 2016;Jarraya et al, 2016;Manley, 2014;Pang et al, 2015) Function Follicle size and count via USG, serum hormone (AMH, LH, FSH, Estradiol) level, physical examination including menstruation period and pregnancy Biopsy sample follicle count/histological evaluation under microscopy or TEM, TUNEL assay, immunohistochemistry and western blot analysis (e.g., Ki-67, Inhibin-α, 3β-HSD, Laminin, Calretinin, P450 aromatase, FSHR antibody, and HMGB1) (J.…”