Short Communication: An association analysis between one missense polymorphism at the SREBF1 gene and milk yield and composition traits in goats

Abstract

. 2012. SHORT COMMUNICATION: An association analysis between one missense polymorphism at the SREBF1 gene and milk yield and composition traits in goats. Can. J. Anim. Sci. 92: 167Á173. Sterol regulatory element binding transcription factor 1 (SREBF1) regulates the expression of genes involved in the biosynthesis of fatty acids and cholesterol. Herewith, we have sequenced the near-complete coding region and part of the 3?UTR of the goat SREBF1 gene. In doing so, we have detected a missense c.353C!T polymorphism causing a proline to leucine substitution at position 118 (P118L). An association analysis with milk composition traits recorded in MurcianoGranadina goats only revealed a statistical tendency linking SREBF1 genotype and milk omega-3 fatty acid content. The lack of significant associations suggests that the P118L substitution does not involve a functional change.Key words: Goat SREBF1 gene, milk fat content and composition Manunza, A., Zidi, A., Ferna´ndez-Cabana´s, V. M., Jordana, J., Carrizosa, J., Belaifa, E., Urrutia, B., Polvillo, O., Gonza´lez-Redondo, P., Amills, M. et Serradilla, J. M. 2012. COMMUNICATION BRE`VE: Analyse d'association entre un polymorphisme non synonyme dans le ge`ne SREBF1 et la production et la composition laitie`res chez les che`vres. Can. J. Anim. Sci. 92: 167Á173. Le facteur de transcription de´nomme´Sterol regulatory element binding transcription factor 1 (SREBF1) re´gule l'expression des ge`nes implique´s dans la biosynthe`se des acides gras et du choleste´rol. Dans cette e´tude, nous avons se´quence´la quasi-totalite´de la re´gion codante et une partie du la re´gion 3?UTR du ge`ne SREBF1 de la che`vre. Ce travail, nous a permis d'identifier un polymorphisme non-synonyme c.353C !T causant la substitution d'une Proline en Leucine al a position 118. L'e´tude d'association avec la composition du lait enregistre´e en che`vres Murciano-Granadina, a re´ve´leś eulement une tendance statistique reliant SREBF1 ge´notype et l'acide gras ome´ga-3 du lait. L'absence d'associations significatives sugge`re que la substitution P118L n'implique pas un changement fonctionnel.

Improving dairy performance through molecular characterization of SREBP-1 gene in Sarda sheep breed

“…These results are in agreement with the findings of Deng et al. (2017) and Manunza et al. (2012) regarding buffalo and goats, respectively.…”

“…The Sarda sheep has been subjected to strong genetic selection in the last century ( Luridiana et al., 2014 ), perhaps leading to the reduction of variations. Furthermore, in other dairy species such as buffalo and goats, few variations in the sequence have been highlighted ( Deng et al., 2017 ; Manunza et al., 2012 ). The low variation in the SREPB-1 gene found in different species evidences the important role of this gene in the milk fat secretion.…”

Buffalo SREBP1: molecular cloning, expression and association analysis with milk production traits

“…Twinning rates in cattle range from 1% to 5% depending on the breed, with dairy breeds typically having the highest rates. [1][2][3][4][5] However, the rate of heteropaternal superfecundation (HS) has so far not been studied, although it receives attention in the popular press. 6-8 HS occurs when two or more ova are fertilized by two or more males during the same reproductive cycle.…”

“…Background: Sterol regulatory element-binding proteins (SREBPs) belong to the basic helix-loop-helix-leucine zipper (bHLH-zipper) family of transcription factors, which are critical regulators of cholesterol and lipid homeostasis. 1 The SREBP1 gene has significant genetic effects on milk fatty acid composition, 2 low-fat milk syndrome, 3 milk composition 4 and milk fat yield. 5 Here we report on water buffalo SREBP1 tissue expression patterns, identify SNPs and investigate the associations between these SNPs and milk traits.…”