Short communication: Bovine mastitis caused by a multidrug-resistant, mcr-1-positive (colistin-resistant), extended-spectrum β-lactamase–producing Escherichia coli clone on a Greek dairy farm

Filioussis, George; Kachrimanidou, Melina; Christodoulopoulos, G.; Kyritsi, Maria; Hadjichristodoulou, Christos; Αδαμοπούλου, Μαρία; Tzivara, A.; Kritas, S. K.; Grinberg, Alex

doi:10.3168/jds.2019-17320

Cited by 41 publications

(28 citation statements)

References 29 publications

(22 reference statements)

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“…These genes have also formerly been detected in isolates from healthy dogs in Greece [30]. We did not detect bla SHV variants, although in our country they have previously been identified in milk samples from cows presenting mastitis and are frequently reported among human strains [31,33].…”

Section: Discussioncontrasting

confidence: 44%

“…In Greece, recently published data indicate overconsumption of antibiotics, as well as rates of antibiotic resistance consistently higher than in other EU member states [24][25][26]. Although ESBL-producing bacteria are frequently detected among humans, reports about animal isolates are scarce [27][28][29][30][31][32]. Both SHV and CTX-M types seem to be common in human strains [33,34], while mainly CTX-M variants have been reported from cattle, poultry, and dogs [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

“…Although ESBL-producing bacteria are frequently detected among humans, reports about animal isolates are scarce [27][28][29][30][31][32]. Both SHV and CTX-M types seem to be common in human strains [33,34], while mainly CTX-M variants have been reported from cattle, poultry, and dogs [30][31][32]. Human ESBL isolates have been detected to co-harbor various other resistance genes, such as plasmid mediated quinolone resistance genes (PMQR), carbapenemase genes and plasmid encoded AmpC genes [35][36][37], whereas animal ESBL isolates have only been correlated with a colistin resistance gene (mcr-1) [31].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

et al. 2021

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The prevalence of multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide. The present study aimed to provide an overview of the multidrug resistance phenotype and genotype of ESBL-producing Escherichia coli (E. coli) isolates of livestock and wild bird origin in Greece. Nineteen phenotypically confirmed ESBL-producing E. coli strains isolated from fecal samples of cattle (n = 7), pigs (n = 11) and a Eurasian magpie that presented resistance to at least one class of non β-lactam antibiotics, were selected and genotypically characterized. A DNA-microarray based assay was used, which allows the detection of various genes associated with antimicrobial resistance. All isolates harbored blaCTX-M-1/15, while blaTEM was co-detected in 13 of them. The AmpC gene blaMIR was additionally detected in one strain. Resistance genes were also reported for aminoglycosides in all 19 isolates, for quinolones in 6, for sulfonamides in 17, for trimethoprim in 14, and for macrolides in 8. The intI1 and/or tnpISEcp1 genes, associated with mobile genetic elements, were identified in all but two isolates. This report describes the first detection of multidrug resistance genes among ESBL-producing E. coli strains retrieved from feces of cattle, pigs, and a wild bird in Greece, underlining their dissemination in diverse ecosystems and emphasizing the need for a One-Health approach when addressing the issue of antimicrobial resistance.

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Section: Discussioncontrasting

confidence: 44%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

et al. 2021

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“…Members of Enterobacteriaceae have been involved in different cases of unresponsiveness to antibiotic treatment including human infections such as community-acquired and foodborne infections. In addition, unresponsive to treatment in animals, especially production diseases such as mastitis (21)(22)(23)(24). In this study, 28.75% (23/80) of raw milk samples were ESBL positive which belongs to apparently healthy cows.…”

Section: Discussionmentioning

confidence: 59%

Detection of CTX-M gene in extended spectrum β-lactamases producing Enterobacteriaceae isolated from bovine milk

Ahmed¹

2021

IJVS

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Extended spectrum β-lactamases producing Enterobacteriaceae (ESBL-E) have emerged recently as the main cause that facilitates the spreading of antibiotic resistance worldwide. Due to its composition and nutritive values, raw cow milk is vulnerable to bacterial contamination from different sources, especially ESBL-E. Accordingly, present study aimed to detect the ESBL-E in the raw milk of healthy cows. 80 raw cow milk samples were collected from unorganized farms and cows belong to individual owners and investigated for the presence of ESBL-E with the main focusing on CTX-M type. The bacterial isolation was performed using selective MacConkey agar plus cefotaxime (MC + ), while PCR was used to confirm the species of the isolated bacteria and presence of CTX-M gene. The results showed that 28.75%(23/80) samples were ESBL-E positive and distributed as following, 82.61%(19/23) were pure E. coli isolates, 4.35%(1/23) was pure K. pneumoniae isolate and finally, 13.04%(3/23) were mixed of both E. coli and K. pneumoniae isolates. Moreover, the total number of positive ESBL-E was 26 isolates with the majority of them were belong to E. coli and recorded 84.61%(22/26), while K. pneumoniae was recorded less 15.39%(4/26). Additionally, the CTX-M gene was successfully identified in all ESBL-E positive isolates by using PCR, including E. coli and K. pneumoniae isolates. The results of this study assert the importance of raw cow milk as a potential source of ESBL-E that might be transmitted to humans.

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“…Moreover, studies performed in European Mediterranean countries highlighted a significant presence of E. coli and Staphylococcus spp. [39][40][41][42]. Moreover, a high incidence of E. coli was also observed among bovine mastitis in India and Taiwan [43,44].…”

Section: Discussionmentioning

confidence: 95%

Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon

et al. 2021

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Mastitis is an infectious disease encountered in dairy animals worldwide that is currently a growing concern in Lebanon. This study aimed at investigating the etiology of the main mastitis-causing pathogens in Northern Lebanon, determining their antimicrobial susceptibility profiles, and identifying their antimicrobial resistance (AMR) genes. A total of 101 quarter milk samples were collected from 77 cows and 11 goats presenting symptoms of mastitis on 45 dairy farms. Bacterial identification was carried out through matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Antimicrobial susceptibility was tested by disc diffusion and broth microdilution methods. Molecular characterization included polymerase chain reaction (PCR) screening for genes encoding extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC among Enterobacterales isolates, and virulence factors among Staphylococcus isolates. Escherichia coli isolates were subjected to phylogenetic typing by a quadruplex PCR method. The most frequently identified species were Streptococcus uberis (19.2%), Streptococcus agalactiae (15.1%), E. coli (12.3%), and Staphylococcus aureus (10.96%). Gram-positive bacteria were resistant to macrolides and tetracycline, whereas gram-negative bacteria displayed resistance to ampicillin and tetracycline. Two ESBL genes, blaTEM (83.3%) and blaOXA (16.7%), and one AmpC beta-lactamase gene, blaCMY-II (16.7%), were detected among six E. coli isolates, which mainly belonged to phylogenetic group B1. Among Staphylococcus spp., the mecA gene was present in three isolates. Furthermore, four isolates contained at least one toxin gene, and all S. aureus isolates carried the ica operon. These findings revealed the alarming risk of AMR in the Lebanese dairy chain and the importance of monitoring antimicrobial usage.

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Short communication: Bovine mastitis caused by a multidrug-resistant, mcr-1-positive (colistin-resistant), extended-spectrum β-lactamase–producing Escherichia coli clone on a Greek dairy farm

Cited by 41 publications

References 29 publications

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

Detection of CTX-M gene in extended spectrum β-lactamases producing Enterobacteriaceae isolated from bovine milk

Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon

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