Short communication: Characterization of the milk protein expression profiles in dairy buffaloes with and without subclinical mastitis

Tanamati, Fernanda; Jf, Taylor; Behura, Susanta K.; Santos, D. R. dos; Stafuzza, Nedenia Bonvino; Andrade, Willian Bruno Fernandes de; Gasparino, Eliane; Tonhati, Humberto

doi:10.3168/jds.2019-16907

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…However, the quarters assigned to the HSCC-L group at the beginning of the trial already displayed higher levels of four antimicrobial proteins compared to the quarters assigned to the HSCC-C group, namely chitinase-3-like protein 1 and CATHL3, CATHL6, CATHL7. Chitinase-3-like protein 1 participates in the innate immune response to microbial pathogens at the site of invasion [31]; its increase has been reported in the milk of cows and buffaloes with mastitis [32,33]. CATHLs are well-known antimicrobial proteins that play a major role in the immune defense of ruminants [34,35].…”

Section: Discussionmentioning

confidence: 99%

Impact of a teat disinfectant based on Lactococcus cremoris on the cow milk proteome

Addis,

Maffioli,

Gazzola

et al. 2023

Preprint

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Background. Dairy cow milking practices require cleaning and disinfection of the teat skin before and after milking to ensure the safety and quality of milk and prevent intramammary infections. Antimicrobial proteins of natural origin can be valuable alternatives to traditional disinfectants. In a recent field trial, we demonstrated that a teat dip based on a nisin A-producing Lactococcus cremoris (L) had comparable efficacy to conventional iodophor dip (C) in preventing dairy cow mastitis. Here, we present the differential shotgun proteomics investigation of the milk collected during the trial. Methods. Four groups of matched quarter milk with low (LSCC) and high somatic cell count (HSCC) collected at the beginning (T0) and end (TF) of the trial were selected, for a total of 28 LSCC and 12 HSCC samples. Milk proteins were digested into peptides, separated by nanoHPLC, and analyzed by tandem mass spectrometry (LC-MS/MS) on an Orbitrap Fusion Tribrid mass spectrometer. The proteins were identified with MaxQuant and interaction networks of the differential proteins were investigated with STRING. The proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD045030. Results. In healthy mlk (LSCC), we detected 90 and 80 differential proteins at T0 and TF, respectively. At TF, the Lactococcus group showed higher levels of antimicrobial proteins. In mastitis milk (HSCC), we detected 88 and 106 differential proteins at T0 and TF, respectively. In the Lactococcus group, 14 proteins with antimicrobial and immune defense functions were enriched at TF vs. 4 proteins at T0. Cathelicidins were among the most relevant enriched proteins. Western immunoblotting validation confirmed the differential abundance. Conclusions. At T0, the proteomic differences observed in healthy milk of the two groups were most likely dependent on physiological variation. On the other hand, antimicrobial and immune defense functions were higher in the milk of cows with mammary gland inflammation of the Lactococcus-treated group. Among other factors, the additional immunostimulatory action of nisin A might play a role in the observed changes.

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Section: Discussionmentioning

confidence: 99%

Impact of a teat disinfectant based on Lactococcus cremoris on the cow milk proteome

Addis,

Maffioli,

Gazzola

et al. 2023

Preprint

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“…Whey fractions contain various proteins such as α-lactalbumin, β-lactoglobulin, lactoferrin, osteopontin (OPN), immunoglobulins, enzymes [including lactoperoxidase (LPO), superoxide dismutase (SOD), platelet-activating factor acetylhydrolase (PAFAH2), and alkaline phosphatase (ALPL)], and growth factors (including transforming growth factor β, insulin-like growth factor, epidermal growth factor, and heparin-binding EGF-like growth factor), which have a range of anti-inflammatory and immune cell regulatory effects . Three crucial milk fractions were significantly altered by mastitis, particularly the proteins linked to host defense and inflammation. − Thus, compared with the abundant caseins, the lower proportion fractions may have a much stronger association with udder inflammation. However, the responses of these fractions to high SCC have not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Bovine Milk Proteome: Milk Fat Globule Membrane Protein Is the Most Sensitive Fraction in Response to High Somatic Cell Count

Yang,

He,

Huan

et al. 2023

J. Agric. Food Chem.

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The impacts of high milk somatic cell count (SCC) on different milk fractions are not well understood. In this study, proteins in milk exosomes, milk fat globule membrane (MFGM), and whey from cows with low (<105 cells/mL, CG) and high SCC (>5 × 105 cells/mL, HSG) were identified using a tandem mass tag proteomic approach. In total, 1568, 2160, and 1002 proteins were identified, with 65, 552, and 98 proteins being altered by high SCC in exosomes, MFGM, and whey, respectively. With high SCC, the exosome marker (ACTB) was increased in the exosomes of HSG. The main MFGM proteins (BTN1A1, PLIN3, FABP3, and MFGE8) and functional proteins (MUC1, IGSF5, TLR5, and CD36/14) were decreased, while the lipid/energy metabolism-related proteins were increased in the MFGM of HSG. The glycolysis-related proteins were increased in the whey of HSG. Also, the host defense/inflammation-related proteins were changed in three fractions under high SCCs. MFGM was the most sensitive fraction to a high SCC, followed by whey. These findings provide guidance for the early detection of unhealthy mammary glands.

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“…Most studies have examined the proteome with a focus on milk quality in food adulteration and nutritional value 19 – 21 . Two recent proteomic studies found changes in the milk proteome in water buffalo with subclinical mastitis 22 , 23 . Transcriptome analysis of milk revealed differences in the transcriptome of somatic cells of Murrah buffaloes and Sahiwal cattle 24 .…”

Section: Introductionmentioning

confidence: 99%

Changes in the lipidome of water buffalo milk during intramammary infection by non-aureus Staphylococci

Ceciliani

Audano

Addis

et al. 2022

Sci Rep

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This study aimed to determine the lipidome of water buffalo milk with intramammary infection (IMI) by non-aureus staphylococci (NAS), also defined as coagulase-negative staphylococci, using an untargeted lipidomic approach. Non-aureus Staphylococci are the most frequently isolated pathogens from dairy water buffalo milk during mastitis. A total of 17 milk samples from quarters affected by NAS-IMI were collected, and the lipidome was determined by liquid chromatography-quadrupole time-of-flight mass spectrometry. The results were compared with the lipidome determined on samples collected from 16 healthy quarters. The study identified 1934 different lipids, which were classified into 15 classes. The abundance of 72 lipids changed in NAS-IMI milk compared to healthy quarters. Significant changes occurred primarily in the class of free fatty acids. The results of this study provided first-time insight into the lipidome of dairy water buffalo milk. Moreover, the present findings provide evidence that NAS-IMI induces changes in water buffalo milk's lipidome.

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Short communication: Characterization of the milk protein expression profiles in dairy buffaloes with and without subclinical mastitis

Cited by 8 publications

References 21 publications

Impact of a teat disinfectant based on Lactococcus cremoris on the cow milk proteome

Impact of a teat disinfectant based on Lactococcus cremoris on the cow milk proteome

Bovine Milk Proteome: Milk Fat Globule Membrane Protein Is the Most Sensitive Fraction in Response to High Somatic Cell Count

Changes in the lipidome of water buffalo milk during intramammary infection by non-aureus Staphylococci

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