Short Communication: Detection of dengue virus antigens in desiccated mosquitoes: an improved tool for surveillance

Thenmozhi, V.; Kabilan, L; Samuel, Philip; Dash, A. P.

doi:10.1111/j.1365-3156.2004.01360.x

Cited by 9 publications

(7 citation statements)

References 5 publications

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“…These chemical vector control programs have limited feasibility mainly due to program maintenance costs [12]. As there is no available prophylactic vaccine for our region (the endemicity here is <50%) [7] and no specific treatment, vector control relies on the effective participation of all people in the area [13]. WHO and the Centers for Disease Control and Prevention recommend and emphasize community educational campaigns that highlight citizens’ responsibility in reducing vector breeding sites [5,14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Health Education on Dengue Fever: A Comparison of Knowledge, Attitude, and Practices in Public and Private High School Children of Jeddah

Usman¹,

Alsahafi²,

Abdulrashid³

et al. 2018

Cureus

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Objective More than half of the world’s population live in areas with a potential risk of acquiring dengue fever (DF). Health education interventions are effective, barring a language communication gap. The objective of this study was to estimate the effect of health education in the knowledge, attitude, and practices (KAP) towards DF control and prevention in public and private schools. Materials and methods We assessed the DF control and prevention strategy KAP of students of eight public and private schools in Jeddah, Saudi Arabia before the dengue health education intervention sessions (pre-I) and three months following the education intervention sessions (post-I) using the same closed-ended validated questionnaire. Schools and students were selected by a multistage stratified random sample method. Statistical analysis was done using the paired and independent T-test in IBM SPSS Statistics for Windows, Version 22.0 (IBM Corp., Armonk, NY). Results We found a significant mean difference in the overall knowledge (pre-I, 7.86 ± 2.61; post-I, 10.94 ± 2.35), attitude (pre-I, 5.16 ± 1.50; post-I 6.23 ± 1.30), and practice (pre-I, 2.96 ± 1.33; post-I, 3.94 ± 1.12) scores. Private schools scored better post-intervention scores in knowledge and practice compared to public schools in local and English language medium. Conclusions Health education programs are essential for DF prevention and management. Institutes whose populations consists of students with various language backgrounds should not be ignored. Bilingual educational sessions are important in such private institutes. Our results indicate additional emphasis is required on putting interventional knowledge into practice.

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Section: Introductionmentioning

confidence: 99%

Effect of Health Education on Dengue Fever: A Comparison of Knowledge, Attitude, and Practices in Public and Private High School Children of Jeddah

Usman¹,

Alsahafi²,

Abdulrashid³

et al. 2018

Cureus

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“…Arboviruses detection in dried mosquitoes has been successful in other systems, such as Japanese encephalitis virus (JEV)/ Culex tritaeniorhynchus [27], JEV/ Culex vishinui but also in DENV/ Ae. aegypti [14,28,29]. These reports, however, employed different experimental designs that hampered potential comparisons between their results and ours.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti females may have influenced virus dynamics and replication in the mosquito body, increasing the success of DENV detection [14,28,30]. Additionally, killing mosquitoes by freezing helped to preserve the virus genome, which also benefited virus detection [14,28,29]. Therefore, by orally infecting mosquito females without freezing, we believe our data may better represent some of the difficulties of DENV surveillance in Ae.…”

Section: Discussionmentioning

confidence: 99%

Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance

et al. 2014

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BackgroundSurveillance is a critical component of any dengue prevention and control programme. Herein, we investigate the efficiency of the commercial kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus (DENV) antigens in Aedes aegypti mosquitoes infected under laboratory conditions.MethodsUnder insectary conditions, four to five day-old mosquitoes were orally challenged with DENV-2 titer of 3.6 x 105 PFU equivalent/ml, incubated for 14 days and then killed. At ten time-points following mosquito death (0, 6, 12, 24, 72, 96, 120, 144 and 168 h), i.e., during a one-week period, dried mosquitoes were comparatively tested for the detection of the NS1 antigen with other methods of detection, such as qRT-PCR and virus isolation in C6/36 cells.ResultsWe first observed that the NS1 antigen was more effective in detecting DENV-2 in Ae. aegypti between 12 and 72 h after mosquito death when compared with qRT-PCR. A second round involved comparing the sensitivity of detection of the NS1 antigen and virus isolation in C6/36 cells. The NS1 antigen was also more effective than virus isolation, detecting DENV-2 at all time-points, i.e., up to 168 h after mosquito death. Meanwhile, virus isolation was successful up to 96 h after Ae. aegypti death, but the number of positive samples per time period presented a tendency to decline progressively over time. From the 43 samples positive by the virus isolation technique, 38 (88.4%) were also positive by the NS1 test.ConclusionTaken together, these results are the first to indicate that the NS1 antigen might be an interesting complementary tool to improve dengue surveillance through DENV detection in dried Ae. aegypti females.

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“…Storing such samples for a long time at room temperature, which increases the number of samples available for a surveillance program (that are often captured using traps), does not interfere with the quality of the results. 24 Since the number of larvae collected can be quite large, the possibility of pooling samples to make large-scale testing could be a feasible alternative.…”

Section: Discussionmentioning

confidence: 99%

Use of immunodot blot and multiplex reverse transcriptase-polymerase chain reaction in dengue virus detection in macerates of Aedes aegypti larvae

Chagas

Oliveira

Mezêncio

et al. 2012

Microbiol Res (Pavia)

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The Dengue virus is the main arbovirus that affects man in terms of morbidity and mortality. The detection of the virus is very important for epidemiological surveillance, so here we propose to standardize and compare the immunodot blot (IDB) and multiplex reverse transcriptase-polymerase chain reaction (M-RT-PCR) techniques to detect and characterize the dengue virus (DENV) serotypes in samples of Aedes aegypti larvae. Thus, the IDB and M-RT-PCR techniques were standardized using macerated samples of larvae collected in nature. The use of monoclonal antibodies in IDB has not shown great results, but DENV detection through this method was possible using polyclonal antibodies. The distinction of serotypes 1, 2 and 3 was carried out by M-RT-PCR

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Short Communication: Detection of dengue virus antigens in desiccated mosquitoes: an improved tool for surveillance

Cited by 9 publications

References 5 publications

Effect of Health Education on Dengue Fever: A Comparison of Knowledge, Attitude, and Practices in Public and Private High School Children of Jeddah

Effect of Health Education on Dengue Fever: A Comparison of Knowledge, Attitude, and Practices in Public and Private High School Children of Jeddah

Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance

Use of immunodot blot and multiplex reverse transcriptase-polymerase chain reaction in dengue virus detection in macerates of Aedes aegypti larvae

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