Short Communication: Genetic identification of local pigs, and imported pigs Duroc) based on cytochrome b sequence analysis

Abstract

Hartatik T, Soewandi BDP, Volkandari SD, Tabun AC, Sumadi, Widodo. 2016. Genetic identification of local pigs, and imported pigs (Landrace and Duroc) based on cytochrome b sequence analysis. . The aim of this study was to identify the genetics of local pigs and imported pigs (Landrace and Duroc) based on qualitative analysis. Thirty-eight pigs were used in this study and consisted of 11 local pigs (from Bali), six Landrace pigs and four Duroc pigs (from Malang), nine Landrace pigs (from Bali) and eight local pigs (from Kupang). Qualitative traits in pigs such as coat color, body shape (back shape, belly shape, and ears) and hair cover were observed. The cytochrome b (Cyt b) gene of mitochondrial DNA was analyzed using PCR-restriction fragment length polymorphism. The PCR analysis resulted in a 464 base pairs (bp) amplified band, and this was digested using TaqI restriction enzyme. The PCR-RFLP analysis resulted in two bands, 246 and 218 bp (monomorphic). The alignment analysis showed four points of single nucleotide polymorphisms. Bali and Kupang pigs had a specific pattern on exterior characteristics such as curved back shape, belly hanging shape, small ears and thick hair, and had many variations on coat color such as black, cream, spotted and mottled. The differences in coat color and body shape, and the corresponding mtDNA Cyt b sequence (with four SNPs) is a marker for genetic variation in pigs.

Short Communication: Genetic diversity and phylogenetic analysis of two Indonesian local cattle breeds based on cytochrome b gene sequences

“…In mtDNA, the cytochrome b (cyt b) gene contains abundant phylogenetic information among intra-and interspecies and it is considered to be a good marker to study the genetic differentiation as well as phylogenetic relationships among species within the same genus or the same family (Browers et al 1994;Zardoya and Meyer 1996). The mtDNA has been proven to be useful markers for elucidating genetic diversity and relationships of domestic animals including chicken, goat, pig, horse, and cattle (Zhu et al 2014 Hartatik et al 2016;Sziszkosz et al 2016;Hartatik et al 2018).…”

Genetic variation within the coat color gene of Melanocortin 1 Receptor (MC1R) in Mong Cai pigs of Vietnam

“…In addition, according to the Domestic Animal Diversity Information System (FAO, http://fao.org/dad-is), the Mong Cai breed shares characteristics with Luchuan pigs, native to China's Guangxi Province (Pham et al 2014;Bui et al 2018). Other evidence from mtDNA and microsatellite studies also revealed that the Mong Cai pig and Vietnamese indigenous pig breeds have a close genetic relationship with Asian pig breeds regarding maternal lineage and are grouped into a separate branch from European breeds (Hartatik et al 2016;Bui et al 2018).…”