Short communication: Germination monitoring of selected Annonaceae seeds: Seed bank collections of Purwodadi Botanic Garden, East Java, Indonesia

Abstract

Abstract. Pratiwi A, Lestari DA, Romdhonah Y. 2022. Short communication: Germination monitoring of selected Annonaceae seeds: Seed bank collections of Purwodadi Botanic Garden, East Java, Indonesia. Biodiversitas 23: 3567-3572. Monitoring activities are an essential aspect to determine the viability and germination of seeds after storage for a certain period. The seed bank of Purwodadi Botanic Garden collected 20 seeds of 11 different species of Annonaceae. Therefore, this study aimed to examine the seed viability of 11 selected species of Annonaceae after being stored for a certain time. Twenty seeds with three replications, i.e., 60 seeds in total were sown. Meanwhile, for genera of Uvaria consisted of U. purpurea, U. rufa, and Uvaria sp. were sown at 30 seeds in total, which means 10 seeds with three replications. The observed parameters were percentage of germination, seed viability, initial germination, and germination type. The results showed only 4 out of 11 selected species of Annonaceae were germinated, namely A. montana, F. latifolium, U. micranthum, and U. purpurea. These four species have epigeal germination types, and monitoring activities included stock rejuvenation, and evaluation of storage procedures for Alphonsea javanica, Annona glabra, Orophea enneandra, Polyalthia littoralis, U. rufa, and Uvaria sp. Furthermore, P. longifolia requires collection regeneration due to its recalcitrant character, and the number of empty seeds from the cut test was >75%. We suggested that P. longifolia seeds might be better to be stored in the refrigerator.

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“…Majority of the ripe fruits are consumed by the local tribals and frugivorous birds and the fallen seeds in stream water perish and do not get germinated (Mohanty and Barik, 2022). It has been clearly mentioned that 26.67 ± 15.28 percentage of germination observed in U. micranthum and 13.33 ± 5.77 percentage of germination observed in U. purpurea (Pratiwi et al, 2022). Seeds of Uvaria acuminata shows 100 percentage of germination being scarified (chipped with scalpel), inoculated on agar; exposed to photoperiod of 16 hrs light vs. 8hours dark at 20-25 0 C whereas seeds of Uvaria acuminata without any kind of treatment have shown 88 percentage of germination (Ferreira et al, 2019).…”

Seed Conservation of <i>Anaxagorea luzonensis</i> A. Gray (Annonaceae) Through Storage Behaviour and Morphology

“…Some species from Annonaceae family have epigeal germination type, such as Annona montana, Artabotrys hexapetalus, Cananga odorata, Fissistigma latifolium, Uvaria purpurea, U. micranthum etc. (Handayani 2019;Pratiwi et al 2022). Based on this study, some of conservation efforts can be carried out with point of view from seed dispersal mechanism, fruit and seed morphology, and seed storage behaviour.…”