Short Communication: Identification of Two Polymorphisms in the Goat Lipoprotein Lipase Gene and Their Association with Milk Production Traits

Badaoui, Bouabid; Serradilla, J. M.; Axelsson, Tomas; Urrutia, B.; Ares, J.; Carrizosa, J.; Sánchez, Armand; Jordana, J.; Amills, Marcel

doi:10.3168/jds.2006-409

Cited by 24 publications

(17 citation statements)

References 18 publications

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“…1984). However, differences in LPL activity may be also a result of polymorphism in the caprine LPL gene that influences the functional properties of the enzyme, as suggested by Badaoui et al. (2007).…”

Section: Lipoprotein Lipase (Lpl)mentioning

confidence: 99%

Indigenous enzymatic activities in ovine and caprine milks

Moatsou¹

2010

Int J of Dairy Tech

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The objective of this review paper is the presentation of research findings about enzymes of ovine and caprine milks taking into consideration the bovine milk indigenous enzymatic activities as a reference. Information about indigenous enzymatic activities in these milk types focuses mainly on plasmin, lipoprotein lipase and on the enzymes that are used as thermal treatment indicators, i.e. alkaline phosphatase, and lactoperoxidase. Further research including the effects of genetic and environmental factors on the enzymatic activities is necessary.

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Section: Lipoprotein Lipase (Lpl)mentioning

confidence: 99%

Indigenous enzymatic activities in ovine and caprine milks

Moatsou¹

2010

Int J of Dairy Tech

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“…Several LPL cDNA and amino acid sequences have also been reported for other vertebrates including gorilla ( Gorilla gorilla ) and rhesus monkey ( Macaca mulatta ) (Martinez et al, 2001), baboon ( Papio anubis ) (Cole & Hixson, 1995), pig ( Sus scrofa ) (Harbitz et al, 1991), cow ( Bos taurus ) (Senda et al, 1987), sheep ( Ovis aries ) (Edwards et al, 1993), cat ( Felis catus ) (Ginzinger et al, 1996), goat ( Capra hercus ) (Badaoui et al, 2007) and guinea pig ( Cavia porcellus ) (Enerbaeck et al, 1987) and fish species, sea bass (Dicentrarchus labrax) (Jose Ilbanez et al, 2008) and bream ( Sparus aurata; Pagrus major ) (Saera-Vila et al, 2005; Oku et al, 2006). LPL genes usually contain 9 exons of DNA encoding LPL sequences which may undergo exon shuffling generating several isoproteins in each case (Thierry-Mieg and Thierry-Mieg, 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparative studies of vertebrate lipoprotein lipase: A key enzyme of very low density lipoprotein metabolism

Holmes

VandeBerg

Cox³

2011

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Lipoprotein lipase (LIPL or LPL; E.C.3.1.1.34) serves a dual function as a triglyceride lipase of circulating chylomicrons and very-low-density lipoproteins (VLDL) and facilitates receptor-mediated lipoprotein uptake into heart, muscle and adipose tissue. Comparative LPL amino acid sequences and protein structures and LPL gene locations were examined using data from several vertebrate genome projects. Mammalian LPL genes usually contained 9 coding exons on the positive strand. Vertebrate LPL sequences shared 58–99% identity as compared with 33–49% sequence identities with other vascular triglyceride lipases, hepatic lipase (HL) and endothelial lipase (EL). Two human LPL N-glycosylation sites were conserved among seven predicted sites for the vertebrate LPL sequences examined. Sequence alignments, key amino acid residues and conserved predicted secondary and tertiary structures were also studied. A CpG island was identified within the 5'-untranslated region of the human LPL gene which may contribute to the higher than average (x4.5 times) level of expression reported. Phylogenetic analyses examined the relationships and potential evolutionary origins of vertebrate lipase genes, LPL, LIPG (encoding EL) and LIPC (encoding HL) which suggested that these have been derived from gene duplication events of an ancestral neutral lipase gene, prior to the appearance of fish during vertebrate evolution. Comparative divergence rates for these vertebrate sequences indicated that LPL is evolving more slowly (2–3 times) than for LIPC and LIPG genes and proteins.

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“…P represents the overall P-value in the regression, and P 1 and P 2 represent the P-value of the slope of mRNA expression of LPL and slope of supply of LCFA by EPA in the regression, respectively. delivery of FA into the mammary gland (Badaoui et al 2007). The milk fat content in the present study significantly increased, and the milk fat of LCFA tended to increase in the goats fed HQR, which were affected by the LPL activity in the mammary gland of the goats.…”

Section: Mammary Metabolismmentioning

confidence: 99%

Effects of different model diets on milk composition and expression of genes related to fatty acid synthesis in the mammary gland of lactating dairy goats

Zhang

Khas-Erdene

et al. 2015

Journal of Dairy Science

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This study examined the effects of different roughage diets on milk composition and the expression of key genes associated with fatty acid (FA) synthesis in the mammary gland of lactating dairy goats. Eight multiparous lactating goats (body weight=43.6±2.5kg, 90±12 d in milk) fitted with external pudic artery and subcutaneous abdominal vein catheters were assigned to 2 treatments in a crossover design. The goats were fed different roughage diets with a similar concentrate-to-roughage ratio. The diets were (1) a high-quality roughage treatment (HQR) containing 28.5% Chinese wildrye hay, 19% corn silage, 9.5% alfalfa, and 43% concentrate or (2) a low-quality roughage treatment (LQR) containing 28% Chinese wildrye hay, 28% corn stover, and 44% concentrate, on a dry matter basis. Each feeding period lasted 21 d. The first 18 d served as an adaptation period, and the last 3 d served as a sample collection period. Dry matter intake, milk yield, and milk composition were measured. Milk and blood samples were collected for FA analysis. Mammary gland biopsies were performed after milking on the last day of each period and the tissues were analyzed for the mRNA expression of acetyl-coenzyme A carboxylase-α (ACACA), FA synthase (FASN), stearoyl CoA desaturase (SCD), and lipoprotein lipase (LPL). Dry matter intake and milk yield were not affected by the treatments. Milk fat (3.16 vs. 2.96%) and protein (2.99 vs. 2.89%) contents were higher in HQR goats than in LQR goats, and milk fat yield tended to be higher in HQR goats (16.7 vs. 15.1g/d). Milk FA composition was not different between treatments, except for C18:3n-3 (0.27 vs. 0.15g/100g). Compared with LQR goats, HQR goats had a higher vein concentration of total FA (0.62 vs. 0.44mg/mL). In HQR goats, the mammary balance of total FA increased (9.17 vs. 5.51g/d), whereas the clearance rate of total FA decreased (103.03 vs. 138.25 L/d). No differences were found in mammary blood flow, artery concentration, and mammary uptake of FA between treatments. Compared with LQR, the expression of FASN and ACACA tended to be increased by 20 and 18%, and the expression of LPL and SCD were increased by 39 and 50% in HQR, respectively. The results demonstrated that diets with HQR can increase milk fat content and yield as well as the expression of LPL and SCD in the mammary gland of dairy goats.

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Short Communication: Identification of Two Polymorphisms in the Goat Lipoprotein Lipase Gene and Their Association with Milk Production Traits

Cited by 24 publications

References 18 publications

Indigenous enzymatic activities in ovine and caprine milks

Indigenous enzymatic activities in ovine and caprine milks

Comparative studies of vertebrate lipoprotein lipase: A key enzyme of very low density lipoprotein metabolism

Effects of different model diets on milk composition and expression of genes related to fatty acid synthesis in the mammary gland of lactating dairy goats

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