Short Communication: Lack of differentiation within the bigeye tuna population of Indonesia

Abstract

Pertiwi NPD, Nugraha B, Kartika R, Sulistyaningsih RK, Jatmiko I, Sembiring A, Mahardini A, Cahyani NKD, Anggoro AW, Madduppa HH, Ambariyanto A, Barber PH, Mahardika GN. 2017. Short Communication: Lack of differentiation within the bigeye tuna population of Indonesia. Biodiversitas 18: 1406-1413. All highly migratory tuna and tuna-like species have vast feeding grounds and spawning grounds. Indonesia’s tuna catch is the largest in the world. However, genetic diversity in the population structure within particular tuna species in Indonesia is very limited. Here we provide genetic data for bigeye tuna (Thunnus obesus) covering fishing grounds and local fish markets throughout Indonesia. A fragment of mitochondrial DNA in the D-loop control region was amplified from samples collected across Indonesia in the biennium 2012-2013. The results showed high haplotype diversity and low nucleotide diversity in our samples. Little differentiation occured between the eleven diverse sampling locations, nor was any separation detected between general regions of Indonesia, nor between samples from fishing grounds and samples from fish markets.

Short Communication: Genetic structure of Longtail Tuna Thunnus tonggol (Bleeker, 1851) in Java Sea, Indonesia

“…This study showed a similar pattern as the bigeye tuna (Thunnus obesus) population in Indonesia which indicated one major clade of bigeye population and minor genetic differences between specific locations (Pertiwi et al 2017). Meanwhile, research conducted by Willette et al (2016) on Longtail Tuna in several locations in South China Sea, i.e.…”

“…In this study, Chelex method was used in DNA extraction method, because this method has been widely known as a simple, non-hazardous, and rapid genome DNA extraction process compared to other methods (Griese and Linder 1994). Chelex methods have been successfully used to extract good quality of DNA and commonly used methods of DNA extraction for genetic study, especially for commercial tuna such as Thunnus obesus (Pertiwi et al 2017), Thunnus albacares (Akbar et al 2014), Auxis thazard, Euthynnus affinis, Katsuwonus pelamis, Rastrelliger kanagurta, and Scomberomorus commerson (Jackson et al 2014). All of the sequences generated from the samples were deposited in the Genbank database (http://www.ncbi.nlm.nih.gov) with accession no.…”

“…A similar study using different species of tunas, i.e. Bigeye Tuna (Thunnus obesus) by Pertiwi et al (2017) in Indonesia, Yellowfin Tuna (Thunnus albacares) by Akbar et al (2014) in Mollucas sea, and Skipjack Tuna (Katsuwonus pelamis) by Manezes et al (2012) in Indian Coast, also showed that several species of tuna that has an indication of high genetic diversity.…”

“…Therefore, in this study, we aim to explore the population genetic structure of Longtail Tuna (T. tonggol) in Java Sea using molecular genetic, which is known as a powerful tool to study population. Understanding this population of T. tonggol in Java Sea could become an important data reference to help the conservation manager and policymaker in managing the sustainable fisheries of this species in the area of study (Pertiwi et al 2017).…”

Analisis Filogenetik Ikan Tuna (Thunnus spp.) yang didaratkan di Pelabuhan Benoa, Bali

“…Walsh et al (1991) menyebutkan bahwa metode ini banyak digunakan karena praktis, lebih cepat, dan tidak melibatkan pelarut organik serta tidak memerlukan banyak transfer tube untuk prosesnya sehingga mendapatkan genome sampel yang menjadi tujuan penelitian dibandingkan metode lainnya. Metode chelex juga telah berhasil diterapkan pada sampel Tuna (Jackson et al, 2014;Iranawati et al, 2016;Pertiwi et al, 2017;Malik et al, 2020).. .…”