Short communication: Markers of oxidant status and inflammation relative to the development of claw lesions associated with lameness in early lactation cows

Abuelo, Ãngel; Gandy, J.C.; Neuder, L.; Brester, Jill L.; Sordillo, Lorraine M.

doi:10.3168/jds.2015-10707

Cited by 28 publications

(30 citation statements)

References 39 publications

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“…Whereas 15-F 2t -isoprostane concentrations in plasma and milk were correlated strictly during the ML period, values of RONS for plasma and milk were correlated throughout all 3 lactation periods, even though milk RONS amounts were 100 times those found in plasma. Nevertheless, these values are consistent with those reported previously using the same assay methodology (Abuelo et al, 2016;Mavangira et al, 2016). The difference in RONS abundance is most likely due to the concentration of the metabolites in milk while they are relatively diluted throughout the body in plasma.…”

Section: Discussionsupporting

confidence: 92%

Production of 15-F-isoprostane as an assessment of oxidative stress in dairy cows at different stages of lactation

Kühn

Mavangira

Gandy

et al. 2018

Journal of Dairy Science

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Oxidative stress contributes to dysfunctional immune responses and predisposes dairy cattle to several metabolic and inflammatory-based diseases. Although the negative effects of oxidative stress on transition cattle are well established, biomarkers that accurately measure oxidative damage to cellular macromolecules are not well defined in veterinary medicine. Measuring 15-F-isoprostane, a lipid peroxidation product, is the gold standard biomarker for quantifying oxidative stress in human medicine. The aim of our study was to determine whether changes in 15-F-isoprostane concentrations in plasma and milk could accurately reflect changes in oxidant status during different stages of lactation. Using liquid chromatography-tandem mass spectrometry, 15-F-isoprostane concentrations were quantified in milk and plasma of 12 multiparous Holstein-Friesian cows that were assigned to 3 different sampling periods, including the periparturient period (1-2 d in milk; n = 4), mid lactation (80-84 d in milk; n = 4), and late lactation (183-215 d in milk; n = 4). Blood samples also were analyzed for indicators of oxidant status, inflammation, and negative energy balance. Our data revealed that 15-F-isoprostane concentrations changed at different stages of lactation and coincided with changes in other gauges of oxidant status in both plasma and milk. Interestingly, milk 15-F-isoprostane concentrations and other indices of oxidant status did not follow the same trends as plasma values at each stage of lactation. Indeed, during the periparturient period, systemic 15-F-isoprostane increased significantly accompanied by an increase in the systemic oxidant status index. Milk 15-F-isoprostane was significantly decreased during the periparturient period compared with other lactation stages in conjunction with a milk oxidant status index that trended lower during this period. The results from this study indicate that changes in 15-F-isoprostane concentrations in both milk and plasma may be strong indicators of an alteration in redox status both systemically and within the mammary gland.

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Section: Discussionsupporting

confidence: 92%

Production of 15-F-isoprostane as an assessment of oxidative stress in dairy cows at different stages of lactation

Kühn

Mavangira

Gandy

et al. 2018

Journal of Dairy Science

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“…As in Caixeta et al (2017), cows were classified retrospectively as having eucalcemia (0 samples with Ca <2.125 mmol/L), subclinical hypocalcemia (SCH; 1 to 3 samples with Ca <2.125 mmol/L), or chronic subclinical hypocalcemia (cSCH; 4 samples with Ca <2.125 mmol/L) between calving and 3 DIM. A subset of plasma samples stored at −80°C were analyzed for oxidant status according to Abuelo et al (2016). To accurately evaluate oxidative stress between the treatment groups, the presence of pro-oxidants and antioxidants needs to be measured (Celi, 2011;Abuelo et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…Because it is difficult to measure individual antioxidants, we used an approach to measure the total antioxidant capacity (Celi, 2011). The antioxidant potential (AOP) was assessed by the reduction of a radical [2,2′ azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); Sigma-Aldrich, St. Louis, MO] as described by Re et al (1999) and Abuelo et al (2016). A 5-point standard curve plus a zero standard was prepared with Trolox (Sigma-Aldrich), a known antioxidant standard.…”

Section: Methodsmentioning

confidence: 99%

“…The interand intraassay CV were 15.5 and 9.0%, respectively. To assess oxidative stress during the transition period, the oxidant status index (OSi; OSi = RONS/AOP) was evaluated because it reflects the changes in redox balance (Abuelo et al, 2013(Abuelo et al, , 2016. For interpretation purposes, a greater OSi indicates a greater risk of oxidative stress.…”

Section: Methodsmentioning

confidence: 99%

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Effects of feeding synthetic zeolite A during the prepartum period on serum mineral concentration, oxidant status, and performance of multiparous Holstein cows

Kerwin

Ryan

Leno

et al. 2019

Journal of Dairy Science

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The objective of this study was to determine the effects of feeding synthetic zeolite A for 3 wk before expected calving on peripartal serum mineral concentrations, hypocalcemia, oxidant status, and performance. Holstein cows (n = 55) entering their second or greater lactations were assigned randomly to 1 of 2 dietary treatments starting 21 d before expected calving: control (CON: 40% corn silage, 33% wheat straw, and 27% concentrate; n = 29) or experimental [EXP: CON plus zeolite A (X-Zelit, Protekta Inc., Lucknow, ON, Canada/Vilofoss, Graasten, Denmark; n = 26) at an inclusion rate of 3.3% of dry matter, targeting 500 g/d as-fed]. Cows were fed the same postpartum diet and housed in individual tiestalls through 28 d in milk. Cows fed EXP had higher serum Ca concentrations as parturition approached and during the immediate postpartum period. Serum P concentrations were lower for the EXP-fed cows during the prepartum period and the first 2 d of lactation, whereas serum Mg concentrations were lower than those of the CON-fed cows only during the immediate periparturient period. Cows fed EXP had decreased prevalence of subclinical hypocalcemia (SCH) from d −1 through 3 relative to day of parturition, with the largest difference occurring within the first day postpartum. Prepartum dry matter intake tended to be decreased and rumination was decreased in cows fed EXP; however; postpartum dry matter intake, rumination, milk yield, milk component yield, and colostrum measurements did not differ between treatments. Cows fed EXP tended to have increased hazard of pregnancy by 150 d in milk when controlling for parity compared with CON-fed cows; potential reproductive benefits merit further study. This study demonstrated that zeolite A supplementation during the prepartum period results in markedly improved serum Ca concentrations around parturition and similar postpartum performance compared with controls and is effective at decreasing hypocalcemia in multiparous Holstein cows.

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“…Lameness is a significant multifactorial disorder [ 6 , 7 , 9 , 14 , 15 , 23 , 27 , 30 , 38 , 41 , 46 , 58 , 59 , 60 , 61 , 62 , 63 ] affecting organic and conventional dairy farms [ 58 , 60 ]. It is mainly at the forefront, due to the increasing prevalence and because no other common dairy disease is associated with such visible symptoms of pain [ 9 , 27 , 60 , 64 ], which may be of a long-term nature.…”

Section: Factors Influencing the Occurrence Of Lamenessmentioning

confidence: 99%

Impact of Nutrients on the Hoof Health in Cattle

Langova

Novotna

Nemcova

et al. 2020

Animals

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Lameness is currently one of the most important and economically demanding diseases in cattle. It is manifested in a change in locomotion that is associated with lesions, especially the pelvic limbs. The disease of the hoof is painful, affecting the welfare of dairy cows. Important factors that influence the health of the limbs include nutrition, animal hygiene, stable technology, and genetic and breeding predispositions. Nutrition is one of the basic preventive factors affecting the quality and growth of the hoof horn, and the associated prevalence of hoof disease. The strength and structure of the hoof horn are affected by the composition of the feed ration (amino acids, minerals, vitamins, and toxic substances contaminating the feed ration, or arising in the feed ration as metabolites of fungi).

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Short communication: Markers of oxidant status and inflammation relative to the development of claw lesions associated with lameness in early lactation cows

Cited by 28 publications

References 39 publications

Production of 15-F-isoprostane as an assessment of oxidative stress in dairy cows at different stages of lactation

Production of 15-F-isoprostane as an assessment of oxidative stress in dairy cows at different stages of lactation

Effects of feeding synthetic zeolite A during the prepartum period on serum mineral concentration, oxidant status, and performance of multiparous Holstein cows

Impact of Nutrients on the Hoof Health in Cattle

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