Short communication: Polymorphism of the <i>GnRHR</i> gene and its association with litter size in Boer goats

Abstract

________________________________________________________________________________ AbstractThe polymorphism in the gonadotropin-releasing hormone receptor (GnRHR) gene was detected by PCR single-strand conformational (PCR-SSCP) and DNA sequencing methods in 224 Boer goats. Two single nucleotide polymorphisms (SNPs), G891T and G757A, were found in the exon I of the goat GnRHR gene and their associations with litter size in Boer goats were evaluated. Association analysis showed that G891T and G757A had significant effects on litter size. In locus G891T, individuals with GT genotype had significantly larger litter sizes compared to GG genotype in the first and third parity. In locus G757A, ewes with GG genotype had significantly higher litter sizes than those of GA in the fourth parity. The present study primarily revealed an association between allele T at 891 locus of the GnRHR gene and high litter size in the first and third parity in Boer goats. These results provide evidence that the GnRHR gene may be significantly correlated with a high prolificacy in goats.

Detection of Genetic Diversity in Four Candidate Genes by Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) Analysis of Goats in Sri Lanka

“…According to studies by Yang et al, (2011) and Kiplagat et al, (2010) respectively on exon 1 of GnRHR gene and exon 4 of k-CSN3 gene indicated that these genes possess only two haplotypes and the results of our study confirm the above finding for Sri Lankan goats by showing only two haplotypes for both genes in Sri Lankan goats as well. Further, findings of this study reveal that both exon 2 of LALBA and intron 2 of BMP4 gene possess three haplotypes that were previously described by Cosenza et al, (2003) and Xing-Tang et al, (2010) respectively.…”

“…The details of the gene fragments, primer pairs used and respective annealing temperatures are given in the Table 2. PCR amplification of LALBA, k-CSN3, BMP4 and GnRHR was done as described by Jain et al, 2009;Kiplagat et al, 2010;Fang et al, 2009 andYang et al, 2011 respectively. PCR reactions were performed in a 25 µl reaction mixture containing 100-50 ng genomic DNA, 0.5 μM of each primer, 10× buffer 2.5 μl, 1.5 mM MgCl2, 200 μM dNTPs, 0.625 units of Taq DNA polymerase and water for each gene.…”

“…LALBA and k-CSN3 genes are known to associate with milk traits (Grosclaude, 1988;Pe´rez-Rodrı´guez et al, 1998;Kumar et al, 2006;Lan et al, 2007;An et al, 2009;Jain et al, 2009 andDettori et al, 2015) such as milk production, composition of milk and cheese making properties. GnRHR gene is a key regulator of the reproduction (MingXing et al, 2009;Yang et al, 2011 andFu et al, 2014) and BMP4 gene is known to significant in growth traits and reproduction (Dijke et al, 2003;Fang et al, 2009 andSharma et al, 2013).…”

Single nucleotide polymorphism of candidate genes in non-descript local goats of Sri Lanka

“…Analysis of the deduced amino acid sequences in A730G and G757A sites showed presence of silent mutations and reported G891T variation is non-synonymous (Table 2). Studies by Yang et al, (2011) have reported the same two SNPs at G757A and G891T. To date, the reported synonymous mutation at A730G was not recorded from any goat breed of the world.…”

“…Many researchers have reported the association between k-CSN3 genetic variability and milk composition (Chiatti et al 2005, Marletta et al 2007 and milk production (Marletta et al 2007). Further studies have revealed the association between LALBA gene polymorphism and nutrient content of milk (Lan 2007, Zidi 2014 and relationship between genetic variability of GnRHR gene and prolificacy trait (Ming-Xing et al 2009, Yang et al 2011. The inclusive goal of this study was to identify SNPs in Caprine k-CSN3, LALBA and GnRHR genes aiming to genetically characterize non-descript local goats of Sri Lanka, thereby to offer genetic clues for future association analysis.…”