Short Communication: Species confirmation of juvenile cloudy grouper, Epinephelus erythrurus (Valenciennes, 1828), based on a morphologic analysis and partial CO1 gene sequencing

Abstract

Abstract. Ariyanti Y, Farajallah A. 2019. Species confirmation of juvenile cloudy grouper, Epinephelus erythrurus (Valenciennes, 1828), based on a morphologic analysis and partial CO1 gene sequencing. Biodiversitas 20: 914-921. The genus Epinephelus is the most species among the genera within the subfamily Epinephelinae. Species identification techniques for groupers, especially in the Epinephelus, are commonly based on color patterns and a suite of morphological characters, including body shape and the size and number of fins, scales and gill rakers. In some species, juveniles are morphologically distinct from adults of the same species, making morphological identification highly problematic. This present work will provide some morphological description or variations of juveniles that have been identified as Epinephelus erythrurus based on CO1 sequences. Further, the present study demonstrates that a molecular genetic technique, based on partial sequencing of the mitochondrial CO1 gene, may be used for the rapid species confirmation of every developmental stage and phase of an organism (juvenile E. erythrurus). Two DNA sequences of E. erythrurus from the Pangandaran District (7o43’8.31”S 108o30’11.59”E) have been submitted to GenBank under accession numbers KP998441 and KP998442.

Phylogenetic analysis of the grouper family (Serranidae) from various local markets in Indonesian waters using COI (cytochrome oxidase I)

“…Grouper fish have potential economic value in the field fishery. However, classification and evolutionary relationships often arise by the number of species that are present remarkable, wide distribution, and lack of morphological keys used in the classification (Ariyanti & Farajallah, 2019). Grouper fish (grouper) generally live in reef ecosystems coral which has important ecological value because it is the main predator in the food chain.…”

Genetic diversity analysis and phylogenetic reconstruction of groupers Epinephelus spp. from Madura Island, Indonesia based on partial sequence of CO1 gene

“…Meanwhile in Indonesia, the application of partial sequence of CO1 gene in several grouper species has generally been reported by Jefri et al (2015) at distant sampling points, in Lampung, Karimunjawa, Madura, Lombok, Tanakeke, Kendari, and Numfor. In addition, grouper sampling at more specific locations was also reported, some of which were in Aceh (Kamal et al 2019), Pangandaran West Java (Ariyanti and Farajallah 2019), and West Papua (Tapilatu et al 2021). Then to complement previous research, this study focuses on observing the genetic diversity and reconstructing the phylogenetic tree of various grouper types (Epinephelus spp.)…”

Grouper DNA barcoding studies in Indonesia: A short review

“…Least Concern Stable (Ariyanti and Farajallah, 2019a;Fadli et al, 2021;Fadli et al, 2020;Jefri et al, 2015;Kusuma, 2018 (Abdullah and Rehbein, 2017;Andriyono and Suciyono, 2020;Antoro et al, 2006;Ariyanti and Farajallah, 2019a;Fadli et al, 2021;Jefri et al, 2015;Santosa et al, 2021) 19 Epinephelus Epinephelus erythrurus AF282, AF283 Least Concern Unknown (Ariyanti and Farajallah, 2019b) 20…”