Short communication: The effect of calcium supplementation at calving on neutrophil function

Miltenburg, Cynthia; Duffield, T.F.; Bienzle, Dorothee; Scholtz, Elizabeth L.; LeBlanc, S.J.

doi:10.3168/jds.2018-14839

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…Surprisingly, despite severe hypocalcemia in response to LPS we observed either no change or actually improved neutrophil function (i.e., MPO activity and oxidative burst). Additionally, Ca infusion had no effect on neutrophil function, which agrees with others (Kehrli and Goff, 1989;Miltenburg et al, 2018). Although our model is acute and is not accompanied with periparturient physiological changes, our results challenge the tenet that hypocalcemia is the primary cause of postcalving immunosuppression (Ducusin et al, 2003;Kimura et al, 2006;Martinez et al, 2014).…”

Section: Discussionsupporting

confidence: 90%

“…Calcium is well known for its roles in regulating leukocyte function (i.e., migration, endothelial adherence, phagocytosis, and so on; Cohen, 1994;Immler et al, 2018). Although not always consistent (Miltenburg et al, 2018), decreased circulating Ca concentrations have been associated with reduced leukocyte activity in cows (Ducusin et al, 2003;Kimura et al, 2006;Martinez et al, 2012;Zhang et al, 2019) and increasing Ca concentrations improved leukocyte activity (Vieira-Neto et al, 2017). Surprisingly, despite severe hypocalcemia in response to LPS we observed either no change or actually improved neutrophil function (i.e., MPO activity and oxidative burst).…”

Section: Discussionmentioning

confidence: 99%

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Effects of maintaining eucalcemia following immunoactivation in lactating Holstein dairy cows

Horst

Mayorga

Al‐Qaisi

et al. 2020

Journal of Dairy Science

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Periparturient hypocalcemia is a common metabolic disorder and it is ostensibly associated with negative health and production outcomes. Acute infection also markedly decreases circulating Ca, but the reasons for and consequences of it on physiological and immunological parameters are unknown. Objectives were to evaluate the effects of maintaining eucalcemia on production, metabolic, and immune variables following an intravenous lipopolysaccharide (LPS) challenge. Twelve multiparous lactating Holstein cows (717 ± 20 kg of body weight; 176 ± 34 d in milk; parity 3 ± 0.2) were enrolled in a study containing 2 experimental periods (P); during P1 (3 d), cows consumed feed ad libitum and baseline values were obtained. At the initiation of P2 (4 d), cows were randomly assigned to 1 of 2 treatments: (1) LPS administered (LPS-Con; 0.5 μg/kg of body weight LPS; n = 6) or (2) LPS administered + eucalcemic clamp (LPS-Ca; 0.5 μg/kg of body weight LPS; Ca infusion; n = 6). Cows were fasted for the first 12 h during P2. After LPS administration, ionized Ca was determined every 15 min for 6 h and every 30 min for an additional 6 h and intravenous Ca infusion was adjusted in LPS-Ca cows to maintain eucalcemia. Blood ionized Ca was decreased 23% for the first 12 h postbolus in LPS-Con cows, and by design, Ca infusion prevented hypocalcemia. To maintain eucalcemia for the 12 h, 13.7 g of Ca was infused. The total Ca deficit (including Ca not secreted into milk) accumulated over the 12 h was 10.4 and 20.2 g for the LPS-Con and LPS-Ca treatments, respectively. Mild hyperthermia (0.8°C) occurred for ~6 h post-LPS administration relative to P1. From 6 to 7 h postbolus rectal temperature from LPS-Ca cows was increased (0.6°C) relative to LPS-Con cows. On d 1 of P2, milk yield decreased (61%) in both treatments relative to P1. Relative to LPS-Con cows, milk yield decreased (15%) in LPS-Ca cows during P2. Overall, circulating LPS-binding protein continuously increased postbolus, and at 24 h LPS-binding protein levels in LPS-Ca cows were increased (80%) relative to LPS-Con cows. During P2, serum amyloid A increased (4-fold) in both treatments relative to P1. Administering LPS initially decreased circulating neutrophils, then cell counts progressively increased with time. Calcium infusion decreased neutrophil counts (40%) from 9 to 12 h postbolus relative to LPS-Con cows. Neutrophil function, as assessed by oxidative burst and myeloperoxidase production, did not differ due to treatment. In summary, maintaining eucalcemia (via intravenous Ca infusion) during an immune challenge appeared to intensify inflammation and adversely affect lactation performance.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Effects of maintaining eucalcemia following immunoactivation in lactating Holstein dairy cows

Horst

Mayorga

Al‐Qaisi

et al. 2020

Journal of Dairy Science

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“…A numerical increase occurred for all measures of PMN function including phagocytosis, oxidative burst percent, phagocytosis intensity, and oxidative burst intensity in 50 g of oral Ca × parity data; however, the increase was not statistically significant. In contrast, Miltenburg et al (2018) did not observe any difference in PMN phagocytosis or oxidative burst at 3 DIM among periparturient animals administered Ca subcutaneously within 12 h of parturition and again 24 h later, compared with control cows given saline subcutaneously. The total amount of Ca delivered over the 2 injections was approximately 9.8 g from Ca gluconate and Ca glucoheptonate.…”

Section: Discussioncontrasting

confidence: 69%

Effects of oral calcium bolus supplementation on intracellular polymorphonuclear leukocyte calcium levels and functionality in primiparous and multiparous dairy cows

Reitsma

Batchelder

Davis

et al. 2020

Journal of Dairy Science

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“…The effect of calcium supplementation or prepartum diet on neutrophil function. We evaluated (Miltenburg et al, 2018b) whether administration of an injectable calcium supplement product at time of calving increased neutrophil oxidative burst or phagocytosis capacity. Cows (n = 27) from four farms were blocked by parity and randomly assigned to receive either a commercial injectable calcium supplement or a placebo within 12 h after calving and again 24 h later.…”

Section: Calciummentioning

confidence: 99%

Review: Relationships between metabolism and neutrophil function in dairy cows in the peripartum period

LeBlanc

2020

Animal

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Aspects of neutrophil function are diminished or dysregulated in dairy cows in the weeks just before and after calving, which appears to be an important contributor to the occurrence of retained placenta, mastitis, metritis and endometritis. The timing and mechanisms by which specific elements of neutrophil function are impaired are only partially understood. Oxidative burst capacity is the element of neutrophil function most consistently shown to be impaired in the week after calving, but that observation may partially be biased because oxidative burst has been studied more than other functions. There is sufficient evidence to conclude that the availability of calcium and glucose, and exposure to elevated concentrations of non-esterified fatty acids or β-hydroxybutyrate affect some aspects of neutrophil function. However, these factors have mostly been studied in isolation and their effects are not consistent. Social stressors such as a competitive environment for feeding or lying space should plausibly impair innate immune function, but when studied under controlled conditions such effects have generally not been produced. Similarly, treatment with recombinant bovine granulocyte colony-stimulating factor consistently produces large increases in circulating neutrophil count with modest improvements in function, but this does not consistently reduce the incidence of clinical diseases thought to be importantly attributable to impaired innate immunity. Research is now needed that considers the interactions among known and putative risk factors for impaired neutrophil function in dairy cows in the transition period.

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Short communication: The effect of calcium supplementation at calving on neutrophil function

Cited by 7 publications

References 17 publications

Effects of maintaining eucalcemia following immunoactivation in lactating Holstein dairy cows

Effects of maintaining eucalcemia following immunoactivation in lactating Holstein dairy cows

Effects of oral calcium bolus supplementation on intracellular polymorphonuclear leukocyte calcium levels and functionality in primiparous and multiparous dairy cows

Review: Relationships between metabolism and neutrophil function in dairy cows in the peripartum period

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