Short Communication: The use of lactose-astaxanthin to maintain the quality of green junglefowl frozen semen

Abstract

Abstract. Bebas W, Agustina KK. 2022. Short Communication: The use of lactose-astaxanthin to maintain the quality of green junglefowl frozen semen.Biodiversitas 23: 5759-5764. The Green Junglefowl is Indonesian germplasm that is at risk of population decline. Therefore, efforts are needed to protect its existence and survival. Cryopreservation of spermatozoa in the form of frozen semen is required to preserve this germplasm. This research aims to determine the effect of lactose-astaxanthin in preventing DNA fragmentation and intact plasma membrane of Green Junglefowl spermatozoa during the freezing process. A completely randomized design with a 2x4 factorial pattern, including two treatment groups and four dimethylsulfoxide (DMSO) concentration factors, was used. The treatment groups are T1 and T2, consisting of egg yolk phosphate diluent and egg yolk phosphate diluent added with a combination of 0.6% lactose-0.004% astaxanthin, respectively. The four DMSO concentration factors were 4%, 6%, 8%, and 10% respectively. Subsequently, each treatment was repeated four times. The DNA fragmentation and intact plasma membrane of spermatozoa were observed, and the data obtained afterward were analyzed for variance, followed by the Ducan test. The results showed that the addition of 0.6% lactose-0.004% astaxanthin with 6% DMSO had a significantly better effect (P<0.05) on the quality of post-thawing spermatozoa with 19.75±0.96% DNA fragmentation and 53.75±3.86% intact plasma membranes. Therefore, it is recommended that a combination of 0.6% lactose-0.004% astaxanthin at 6% DMSO concentration should be added to Green Junglefowl semen during the freezing process to prevent DNA fragmentation and intact plasma membranes.

Effects of Adding Glutathione to AndroMed Diluent on Intact Plasma and Acrosome Membranes, and Progressive Motility of Cattle Spermatozoa During Freezing Processes

“…The results from this research (Table 1) were consistent with Bebas et al (2022), where macroscopic evaluation such as volume, odor, consistency, color, and pH was reported as 5.98±1.35 (Bebas and Agustina, 2022). Meanwhile, microscopic evaluation, including mass motility, concentration, progressive motility, abnormality, and IPM was noted as +++, 1104 ± 202.21 x 10 6 ml -1 of ejaculate, 69.58±0.30%, 4.66±1.58%, and 83.22±1.64%, respectively.…”

“…The success of an artificial insemination program using frozen semen depends on the quantity and quality of semen ejaculated by a male and the freezing process. This process begins with semen collection, evaluation, dilution, filling and sealing, equilibration, aerating over liquid nitrogen vapor, immersion in liquid nitrogen, and thawing (Bebas et al, 2018;Bebas and Agustina, 2022).…”

“…Cold shock damages the plasma and acrosome membranes (Ax et al, 2000;Bebas et al, 2018). Another primary issue faced during semen freezing is the exposure of spermatozoa to free radicals (Zhang et al, 2021;Bebas and Agustina, 2022). During this process, spermatozoa undergo peroxidation that generates free radicals such as hydroxyl (-OH) and singlet oxygen or 1 [O] 2 (Bansal and Bilaspuri, 2011;Park and Yu, 2017).…”

“…During this process, spermatozoa undergo peroxidation that generates free radicals such as hydroxyl (-OH) and singlet oxygen or 1 [O] 2 (Bansal and Bilaspuri, 2011;Park and Yu, 2017). These radicals are highly reactive and potentially induce lipid peroxidation in the plasma and acrosome membranes (Douard et al, 2003;Bebas and Agustina, 2022). Lipid oxidation in the plasma membrane produces malondialdehyde (MDA) as a marker for the presence of toxic free radicals, which reduces motility and causes DNA damage (Dutta et al, 2019).…”

Penambahan Vitamin E pada Pengencer SemenLife® Terhadap Kualitas dan Lama Simpan Spermatozoa Babi Landrace

“…Kerusakan DNA spermatozoa yang terjadi selama penyimpanan dalam suhu dingin disebabkan oleh peningkatan kadar ROS. Selama proses penyimpanan spermatozoa pada suhu dingin, aktivitas metabolisme tetap aktif yang secara fisiologis menghasilkan senyawa oksigen reaktif, oksigen berperan penting dalam proses metabolisme dengan menghasilkan energi di dalam mitokondria melalui reaksi redoks, yaitu mengoksidasi molekul sehingga menghasilkan Adenosine Tri Phosphate (ATP) sebagai sumber energi (Bebas et al, 2022). Selama proses ini, oksigen memiliki beberapa turunan radikal bebas atau ROS (Juan et al, 2021;Martemucci et al, 2022).…”