Short Communication: Urinary excretion of glutamine transaminase K as an early index of mercuric chloride-induced nephrotoxicity

Trevisan, Andrea; Nicoletto, Giampaolo; Secondin, Livia; Maso, Stefano

doi:10.3109/13547509609079348

Cited by 2 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Angiotensin-converting enzyme is a brush-border enzyme distributed along the whole proximal tubule, even though its activity is prevalently in the pars recta. 22 Owing to its localization in the brush border, it shows high sensitivity to acute and chronic tubulotoxicity caused by chemicals such as mercuric chloride 23 and cadmium. 24 Naive male rats (115 samples) showed a wide range of urinary values (8.9-63.7 µmol mmol −1 creatinine and 59.4-282.7 nmol h −1 ).…”

Section: Resultsmentioning

confidence: 99%

Historical control data on urinary and renal tissue biomarkers in naive male Wistar rats

Trevisan

Giraldo

Borella

et al. 2001

J of Applied Toxicology

View full text Add to dashboard Cite

Total proteins, angiotensin-converting enzyme, N-acetyl-beta-D-glucosaminidase, glutamine transaminase K and glutamine synthetase were determined in urine collected overnight (14 h: 6:00 p.m.-8:00 a.m.) from naive male Wistar rats; glutamine transaminase K and glutamine synthetase in the kidney 10,000 g supernatant and p-aminohippurate uptake in renal cortical slices also were measured. Urinary parameters were related both to urinary creatinine concentration and urinary flow rate; kidney parameters were related to protein concentration (enzymes) or slice/medium (S/M) ratio (p-aminohippurate uptake). The following reference ranges (1.0 and 99.0 percentiles) were obtained: urine: total urinary proteins (195 samples) 0.03-0.29 g mmol(-1) creatinine and 0.13-1.77 mg h(-1); angiotensin-converting enzyme (115 samples) 8.9-63.7 micromol mmol(-1) creatinine and 59.4-282.7 nmol h(-1); glutamine transaminase K (115 samples) 0-1.7 micromol mmol(-1) creatinine and 0-8.5 nmol h(-1); N-acetyl-beta-D-glucosaminidase (72 samples) 0.7-5.0 micromol mmol(-1) creatinine and 4.9-28.4 nmol h(-1) (naive male rats did not excrete glutamine synthetase); kidney: glutamine transaminase K (36 samples) 14.5-32.8 nmol mg(-1) protein; glutamine synthetase (22 samples) 13.9-48.6 nmol mg(-1) protein and p-aminohippurate (54 samples) 4.77-17.89 S/M. Urinary creatinine (r = -0.780), total urinary proteins (r = -0.521), angiotensin-converting enzyme (r = -0.650) and N-acetyl-beta-D-glucosaminidase (r = -0.796) but not glutamine transaminase K were well correlated with diuresis. In addition, the same parameters, but not glutamine transaminase K, were well correlated with creatinine (r = 0.604,0.701 and 0.747, respectively). Significant correlation also was observed between urinary indices adjusted to creatinine or urinary flow rate (total urinary proteins: r = 0.813; angiotensin-converting enzyme: r = 0.677; glutamine transaminase K: r = 0.939; N-acetyl-beta-D-glucosaminidase: r = 0.657). Finally, a low but significant correlation was found between total urinary proteins and angiotensin-converting enzyme (r = 0.293) and N-acetyl-beta-D-glucosaminidase (r = 0.471).

show abstract

Section: Resultsmentioning

confidence: 99%

Historical control data on urinary and renal tissue biomarkers in naive male Wistar rats

Trevisan

Giraldo

Borella

et al. 2001

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

Kidney and liver biomarkers in female dry-cleaning workers exposed to perchloroethylene

Trevisan

Macca²,

Rui³

et al. 2000

Biomarkers

View full text Add to dashboard Cite

Blood and urine perchloroethylene and urine trichloroacetic acid, as markers of exposure, and serum AST, ALT, GGT and creatinine, urine total solutes and proteins, angiotensin converting enzyme, N-acetyl-ß-D-glucosaminidase and glutamine synthetase, as markers of effect, were measured in 40 dry-cleaning and 45 ironing-shop female workers. Average perchloroethylene air level in the dry-cleaning shops was 59.7 mg m(-3), i.e. three-fold lower than the current A.C.G.I.H. TLV-TWA (170 mg (m-3)). No statistically significant difference in the mean values of any of the effect markers was observed between the two groups, except for AST which was significantly higher in drycleaners. In addition, a statistically significant correlation was observed in dry-cleaners between environmental perchloroethylene and total urinary solutes (r = 0.308, p < 0.05) or urine glutamine synthetase (r= 0.469, p < 0 .01), between glutamine synthetase and blood perchloroethylene in post-shift (r= 0.406, p < 0.01) or urinary perchloroethylene in post(r= 0.571, p < 0.001) or pre-shift (r= 0.586, p < 0.001), and between urinary perchloroethylene in pre-shift and GGT (r= 0.407, p < 0.05). Interestingly, some statistically significant correlations between exposure and effect indices were found in ironing-shop workers alone, as in all subjects. Finally, transaminases, GGT and total urinary proteins were influenced by age and alcohol consumption which were significantly higher in dry-cleaners, thus providing an explanation for some of the correlations observed. In conclusion, our results show a dose-related increase of glutamine synthetase activity,a marker of damage of the pars recta of the kidney proximal tubule, in the urine of female subjects exposed to perchloroethylene concentrations in the work environment lower than current A.C.G.I.H. TLV-TWA.

show abstract

Short Communication: Urinary excretion of glutamine transaminase K as an early index of mercuric chloride-induced nephrotoxicity

Cited by 2 publications

References 19 publications

Historical control data on urinary and renal tissue biomarkers in naive male Wistar rats

Historical control data on urinary and renal tissue biomarkers in naive male Wistar rats

Kidney and liver biomarkers in female dry-cleaning workers exposed to perchloroethylene

Contact Info

Product

Resources

About