Short exposure to cadmium disrupts the olfactory system of zebrafish (Danio rerio) – Relating altered gene expression in the olfactory organ to behavioral deficits

Volz, Sina; Hausen, Jonas; Nachev, Milen; Ottermanns, Richard; Schiwy, Sabrina; Hollert, Henner

doi:10.1016/j.aquatox.2020.105555

Cited by 17 publications

(14 citation statements)

References 67 publications

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“…Cd Exposure Altered the Expression Profile of SiOBPs. An increasing number of studies have suggested that heavy metal contamination alters the expression of olfactory genes in zebrafish, 22 while the effect of chronic heavy metal ingestion on the transcription of olfactory genes in arthropods has not been elucidated. Our previous study demonstrated that 11 of the 16 detected SiOBPs showed transcriptional activation in S. invicta from exposure to 300 mg Cd kg −1 , but SiOBP7 and SiOBP9 displayed transcriptional inhibition across the tested Cd concentrations, 27 suggesting a dose-dependent effect and responsive specificity of SiOBPs.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Currently, most studies are focused on the olfactory toxicity and the transcriptional alteration of olfactory markers evoked by short-term metal exposure. [18][19][20]22,28 However, the long-term effect and the underlying molecular mechanism by which heavy metal exposure alters the expression of olfactory genes remain unclear. We previously reported that dietary exposure at different Cd concentrations resulted in rapid Cd accumulation and inordinate OBP transcriptions in S. invicta, 27 suggesting that SiOBPs are responsive to Cd exposure.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Similarly, Cu exposure changed the expression patterns of olfactory marker proteins and miRNAs involved in olfactory signal transduction in zebrafish, , suggesting complex variations in chemosensory perception of fish to odorants with exposure to heavy metal contamination. Currently, most studies are focused on the olfactory toxicity and the transcriptional alteration of olfactory markers evoked by short-term metal exposure. − ,, However, the long-term effect and the underlying molecular mechanism by which heavy metal exposure alters the expression of olfactory genes remain unclear. We previously reported that dietary exposure at different Cd concentrations resulted in rapid Cd accumulation and inordinate OBP transcriptions in S. invicta , suggesting that SiOBPs are responsive to Cd exposure.…”

Section: Introductionmentioning

confidence: 99%

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Sublethal Exposure to Cadmium Induces Chemosensory Dysfunction in Fire Ants

Yang

Zhang

Liu

et al. 2022

Environ. Sci. Technol.

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Ants easily accumulate cadmium (Cd) from the food web in terrestrial ecosystems. Cd contamination may cause olfactory dysfunction and consequently disorders in the social behavior of ants. To explore the molecular mechanism underlying the effect of Cd exposure on the chemosensory process of ants, we characterized the Cd-induced variations in the expression of genes involved in chemoreception and electrophysiological and behavioral sensitivity to semiochemicals by using the red imported fire ant, Solenopsis invicta, as a model system. As a result, Cd exposure increased Cd accumulation and decreased the survival rate of S. invicta. Cd exposure altered the expression profiles of odor binding protein genes of S. invicta (SiOBPs). Specifically, SiOBP15 protein expression was upregulated upon Cd exposure. Both SiOBP7 and SiOBP15 exhibited high binding affinities to limonene, nonanal, and 2,4,6-trimethylpyridine. S. invicta exposed to Cd showed less sensitive electrophysiological and behavioral response to the three chemicals but exhibited sensitive perception to undecane. Silencing of SiOBP7 and SiOBP15 abolished the behavioral response of S. invicta to nonanal and undecane, respectively, suggesting that SiOBP7 and SiOBP15 play essential roles in the chemoreception of S. invicta. In general, our results suggest that Cd contamination may interfere with olfactory signal transduction by altering the expression of SiOBPs, consequently evoking chemosensory dysfunction in fire ants.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sublethal Exposure to Cadmium Induces Chemosensory Dysfunction in Fire Ants

Yang

Zhang

Liu

et al. 2022

Environ. Sci. Technol.

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“…To investigate whether the measured responses were mediated by olfaction, we treated larvae with cadmium chloride prior to measuring their responses to insecticides. Cadmium is an established olfactory toxicant and known to cause ablation of ciliary and microvillous olfactory sensory neurons (OSNs) without negatively affecting locomotor ability (Supporting Information, Figure S7). − Additional ablation of mechanosensory hair cells of the lateral line, however, cannot be excluded . A batch of approximately 50 larvae (5 dpf) was exposed to a 1 μM (0.18 mg/L) solution of the metal salt cadmium chloride for 24 h [adapted from Heffern et al (2018)] .…”

Section: Methodsmentioning

confidence: 99%

Sub-Lethal Peak Exposure to Insecticides Triggers Olfaction-Mediated Avoidance in Zebrafish Larvae

Könemann

Meyer

Betz

et al. 2021

Environ. Sci. Technol.

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In agricultural areas, insecticides inevitably reach water bodies via leaching or run-off. While designed to be neurotoxic to insects, insecticides have adverse effects on a multitude of organisms due to the high conservation of the nervous system among phyla. To estimate the ecological effects of insecticides, it is important to investigate their impact on non-target organisms such as fish. Using zebrafish as the model, we investigated how different classes of insecticides influence fish behavior and uncovered neuronal underpinnings of the associated behavioral changes, providing an unprecedented insight into the perception of these chemicals by fish. We observed that zebrafish larvae avoid diazinon and imidacloprid while showing no response to other insecticides with the same mode of action. Moreover, ablation of olfaction abolished the aversive responses, indicating that fish smelled the insecticides. Assessment of neuronal activity in 289 brain regions showed that hypothalamic areas involved in stress response were among the regions with the largest changes, indicating that the observed behavioral response resembles reactions to stimuli that threaten homeostasis, such as changes in water chemistry. Our results contribute to the understanding of the environmental impact of insecticide exposure and can help refine acute toxicity assessment.

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“…Animal studies have con rmed metal-induced olfactory impairment, especially Cd-related damage. A German study reported that Cd exposure could impair olfaction in zebra sh and further disrupt their antipredator response(Volz et al 2020). Another study reported that Cd and Zn, but not As or Cr, could inhibit zebra sh olfaction at environmental concentrations(Heffern et al 2018).…”

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confidence: 99%

Insights into the health status of the general population living near an electroplating industry zone: metal elevation and renal impairment

Lai

et al. 2022

Preprint

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To evaluate the body burdens of metals and metalloids associated with renal dysfunction in residents living near electroplating industries, a cross-sectional study was conducted in 2016 in Zhejiang Province, China. We recruited 236 subjects and performed physical examinations; determined the blood and urinary levels of arsenic (As), cadmium (Cd), chromium (Cr), manganese (Mn), nickel (Ni), lead (Pb), antimony (Sb), and selenium (Se) by an inductively coupled plasma mass spectrometer (ICP-MS); and measured three renal impairment biomarkers, namely Nacetyl-β-D-glucosaminidase (NAG), retinol binding protein (RBP), and β2-microglobulin (BMG). The proportion of abnormal nasal symptoms in the exposure group (10.1%) was much higher than in the control group (0; p < 0.05). Both the blood and urinary levels of As, Cd, and Se in the exposure group were significantly higher than the levels in the control group (p < 0.05). The blood levels of Mn and Pb, as well as the urinary levels of Cr and Ni, in the exposure group were significantly higher compared to the control group (p < 0.05). The exposure group demonstrated higher levels of NAG, RBP, and BMG compared to the control group (0.51 vs. 0.14 mg/g creatinine, 12.79 vs. 9.26 IU/ g creatinine, 1.39 vs. 0.78 mg/g creatinine, respectively; p < 0.05). Urinary BMG was positively correlated with urinary Cd levels (r = 0.223, p < 0.05), while urinary RBP was correlated with blood Cd levels (r = 0.151, p < 0.05) and urinary Cd, Cr, Ni, and Se levels (r = 0.220, 0.303, 0.162, and 0.306, respectively; p < 0.05). In conclusion, our study indicated that residents living in the vicinity of electroplating industries were bearing high body burdens of certain metals and metalloids associated with nonnegligible renal dysfunction.

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Short exposure to cadmium disrupts the olfactory system of zebrafish (Danio rerio) – Relating altered gene expression in the olfactory organ to behavioral deficits

Cited by 17 publications

References 67 publications

Sublethal Exposure to Cadmium Induces Chemosensory Dysfunction in Fire Ants

Sublethal Exposure to Cadmium Induces Chemosensory Dysfunction in Fire Ants

Sub-Lethal Peak Exposure to Insecticides Triggers Olfaction-Mediated Avoidance in Zebrafish Larvae

Insights into the health status of the general population living near an electroplating industry zone: metal elevation and renal impairment

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