Short Interfering RNA Accumulation Correlates with Host Recovery in DNA Virus-Infected Hosts, and Gene Silencing Targets Specific Viral Sequences

Padmanabhan, Chellappan; Ramachandran, Vanitharani; Pita, Justin S.; Fauquet, Claude

doi:10.1128/jvi.80.2.1064.2006

Cited by 42 publications

(63 citation statements)

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“…This was mainly attributed to the less permissive nature of these hosts because otherwise the clones are highly infectious on other legumes after agroinoculation. Delivery of viral constructs into the original host has also posed problems for Potato yellow mosaic virus (Buragohain et al 1994) and Indian cassava mosaic virus (Chellappan et al 2004). Many begomoviruses infect Nicotiana benthamiana, which serves as their assay host.…”

Section: Discussionmentioning

confidence: 99%

Infectivity analysis of a soybean isolate of Mungbean yellow mosaic India virus by agroinoculation

et al. 2005

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Yellow mosaic disease (YMD) of legumes endemic to South Asia are caused by begomoviruses transmitted by whiteflies. Based on molecular characterization, two distinct viruses -Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) -were found previously to be the etiological agents of YMD in legumes. Here, host range studies with a soybean isolate of MYMIV (MYMIV-[Sb]) were carried out by both whitefly transmission and agroinoculation. MYMIV-[Sb] was similar to a cowpea isolate of MYMIV (MYMIV-[Cp]) in its ability to infect cowpea, thus differing from blackgram (MYMIV) and mungbean (MYMIV-[Mg]) isolates, which do not infect cowpea. Genomic analysis of DNA A and DNA B components of these MYMIV isolates show characteristic differences in complete DNA B nucleotide sequence correlating with host range differences.

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Section: Discussionmentioning

confidence: 99%

Infectivity analysis of a soybean isolate of Mungbean yellow mosaic India virus by agroinoculation

et al. 2005

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“…The transcription is bidirectional with two major polycistronic transcripts in opposite orientations occurring from the CR that contains the bidirectional promoter sequences. The virion sense AV2-AV1 (CP) transcript and the complementary sense AC1-AC3 transcript overlap by 4 bp at their 3 0 ends as demonstrated by Chellappan et al (2004). It was therefore suggested that the overlapping transcripts in opposite polarity at the 3 0 end might generate dsRNA due to complementary base pairing, which could induce PTGS (Voinnet 2001).…”

Section: Functions Of Rna-silencing Suppressor Proteinsmentioning

confidence: 95%

“…Several groups have independently demonstrated the potent silencing suppressor activity of AC2 and AC4 from a number of different geminiviruses using agroinfiltration assays (Voinnet et al 1999;Vanitharani et al 2004;Trinks et al 2005). Chellappan et al (2004) demonstrated that in case of African cassava mosaic virus-[CM] (ACMV-[CM])-infected plants; the presence of virus specific siRNA promotes the degradation of the corresponding miRNA in a sequencespecific manner, which in turn affects viral replication and transcription. As a result, virus titer and symptom development was greatly reduced in new leaves, indicative that the viral suppressor is important in determining recovery phenotypes (Szittya et al 2002).…”

Section: Bipartite Begomovirusesmentioning

confidence: 99%

RNA-silencing suppressors of geminiviruses

Sharma

Ikegami

2008

J Gen Plant Pathol

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Geminiviruses are single-stranded (ss) DNA viruses that not only cause devastating diseases of important food and fiber crops worldwide, but also are important models to study fundamental aspects of virus-induced gene silencing and RNA interference. As a counterdefense mechanism, viruses have evolved various antisilencing strategies that are being progressively unraveled. The geminiviruses, ssDNA molecules that replicate inside the nucleus and therefore have no dsRNA phase during replication, can both induce and become targets of gene silencing. Proteins AC2 (encoding the transcriptional activator protein) and AC4 of bipartite geminiviruses and protein C2, a positional homolog of AC2 of monopartite viruses, have been identified as suppressors of posttranscriptional gene silencing. The majorities of geminiviral suppressors characterized to date do not share any obvious structural or sequence similarity across families and groups except that they have been identified as pathogenicity determinants. This review mainly focuses on the geminivirus-encoded suppressors of RNA-silencing-the bC1 and V2 proteins-and their possible role in the interference of silencing at different steps in the pathways.

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“…We used two sequences from putative overlapping transcripts (V1-V2 and C1-C2) [39] and a third sequence from the IR promoter. Small-RNA analysis of TYLCV-infected tomato indeed showed the accumulation of v-siRNA from the overlapping TYLCV RNA V1-V2 and C1-C2 regions, but not from the IR section (Fig.…”

Section: Size and Distribution Of T-sirna In Transgenic Rootstock Andmentioning

confidence: 99%

Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

et al. 2015

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Gene silencing is a natural defense response of plants against invading RNA and DNA viruses. The RNA post-transcriptional silencing system has been commonly utilized to generate transgenic crop plants that are "immune" to plant virus infection. Here, we applied this approach against the devastating DNA virus tomato yellow leaf curl virus (TYLCV) in its host tomato (Solanum lycopersicum L.). To generate broad resistance to a number of different TYLCV viruses, three conserved sequences (the intergenic region [NCR], V1-V2 and C1-C2 genes) from the genome of the severe virus (TYLCV) were synthesized as a single insert and cloned into a hairpin configuration in a binary vector, which was used to transform TYLCV-susceptible tomato plants. Eight of 28 independent transgenic tomato lines exhibited immunity to TYLCV-Is and to TYLCV-Mld, but not to tomato yellow leaf curl Sardinia virus, which shares relatively low sequence homology with the transgene. In addition, a marker-free (nptII-deleted) transgenic tomato line was generated for the first time by Agrobacterium-mediated transformation without antibiotic selection, followed by screening of 1180 regenerated shoots by whitefly-mediated TYLCV inoculation. Resistant lines showed a high level of transgene-siRNA (t-siRNA) accumulation (22% of total small RNA) with dominant sizes of 21 nt (73%) and 22 nt (22%). The t-siRNA displayed hot-spot distribution ("peaks") along the transgene, with different distribution patterns than the viral-siRNA peaks observed in TYLCV-infected tomato. A grafting experiment demonstrated the mobility of 0.04% of the t-siRNA from transgenic rootstock to non-transformed scion, even though scion resistance against TYLCV was not achieved.

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Short Interfering RNA Accumulation Correlates with Host Recovery in DNA Virus-Infected Hosts, and Gene Silencing Targets Specific Viral Sequences

Cited by 42 publications

References 0 publications

Infectivity analysis of a soybean isolate of Mungbean yellow mosaic India virus by agroinoculation

Infectivity analysis of a soybean isolate of Mungbean yellow mosaic India virus by agroinoculation

RNA-silencing suppressors of geminiviruses

Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

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