Short interfering RNA-mediated knockdown of drosha and pasha in undifferentiated Meloidogyne incognita eggs leads to irregular growth and embryonic lethality

Dalzell, Johnathan J.; Warnock, Neil D.; Stevenson, Michael; Mousley, Angela; Fleming, Colin C.; Maule, Aaron G.

doi:10.1016/j.ijpara.2010.03.010

Cited by 32 publications

(36 citation statements)

References 24 publications

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“…Surprisingly, 7 siRNAs (Minc03313, Minc03866, Minc05001, Minc10706, Minc14652, Minc17713, Minc07817) induced significant and reproducible increase in transcript abundance when qPCR was performed 24 h after soaking. A similar ‘bounce’ effect, that could be due to a response of the nematode RNAi machinery to increased siRNA quantities within the cells, had already been reported in PPN after gene silencing [21], [22], [23]. To test this ‘bounce’ effect, we performed qPCR analysis 16 h after soaking on Minc07817, Minc03866 and Minc05001.…”

Section: Resultsmentioning

confidence: 57%

“…It is possible that some of the six other genes that showed an increase of transcripts level at 24 h may also present an initial decrease at an earlier time point. Such bounce phenomenon has already been described in plant-parasitic nematodes [21], [22], [23]. Interestingly, the 13 siRNAs yielding effects on transcript level encompass 10 out of the 12 cases of reproducible significant reduction of infestation.…”

Section: Discussionmentioning

confidence: 62%

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Identification of Novel Target Genes for Safer and More Specific Control of Root-Knot Nematodes from a Pan-Genome Mining

et al. 2013

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Root-knot nematodes are globally the most aggressive and damaging plant-parasitic nematodes. Chemical nematicides have so far constituted the most efficient control measures against these agricultural pests. Because of their toxicity for the environment and danger for human health, these nematicides have now been banned from use. Consequently, new and more specific control means, safe for the environment and human health, are urgently needed to avoid worldwide proliferation of these devastating plant-parasites. Mining the genomes of root-knot nematodes through an evolutionary and comparative genomics approach, we identified and analyzed 15,952 nematode genes conserved in genomes of plant-damaging species but absent from non target genomes of chordates, plants, annelids, insect pollinators and mollusks. Functional annotation of the corresponding proteins revealed a relative abundance of putative transcription factors in this parasite-specific set compared to whole proteomes of root-knot nematodes. This may point to important and specific regulators of genes involved in parasitism. Because these nematodes are known to secrete effector proteins in planta, essential for parasitism, we searched and identified 993 such effector-like proteins absent from non-target species. Aiming at identifying novel targets for the development of future control methods, we biologically tested the effect of inactivation of the corresponding genes through RNA interference. A total of 15 novel effector-like proteins and one putative transcription factor compatible with the design of siRNAs were present as non-redundant genes and had transcriptional support in the model root-knot nematode Meloidogyne incognita. Infestation assays with siRNA-treated M. incognita on tomato plants showed significant and reproducible reduction of the infestation for 12 of the 16 tested genes compared to control nematodes. These 12 novel genes, showing efficient reduction of parasitism when silenced, constitute promising targets for the development of more specific and safer control means.

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Section: Resultsmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 62%

Identification of Novel Target Genes for Safer and More Specific Control of Root-Knot Nematodes from a Pan-Genome Mining

et al. 2013

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“…Short interference RNAs (siRNAs) have also been used to knockdown gene-specific FLP transcripts in G. pallida and M. incognita J2 (Dalzell et al 2010). The variability in the degradation enzymes between a free-living nematode, Panagrellus redivivus, and M. incognita have recently been investigated (Masler 2010).…”

Section: Neuropeptidesmentioning

confidence: 99%

Neurobiology of plant parasitic nematodes

Holden‐Dye

Walker

2011

Invert Neurosci

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The regulatory constraints imposed on use of chemical control agents in agriculture are rendering crops increasingly vulnerable to plant parasitic nematodes. Thus, it is important that new control strategies which meet requirements for low toxicity to non-target species, vertebrates and the environment are pursued. This would be greatly facilitated by an improved understanding of the physiology and pharmacology of these nematodes, but to date, these microscopic species of the Phylum Nematoda have attracted little attention in this regard. In this review, the current information available for neurotransmitters and neuromodulator in the plant parasitic nematodes is discussed in the context of the more extensive literature for other species in the phylum, most notably Caenorhabditis elegans and Ascaris suum. Areas of commonality and distinctiveness in terms of neurotransmitter profile and function between these species are highlighted with a view to improving understanding of to what extent, and with what level of confidence, this information may be extrapolated to the plant parasitic nematodes.

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“…Today, RNAi has become an established experimental technique in a number of parasitic nematodes to investigate the function of different genes. The successful application of RNAi as tool for functional genomics has also been demonstrated in plant parasitic nematodes including the sedentary endoparasitic nematodes Globodera pallida, Heterodera glycines and Meloidogyne incognita [10][11][12][13] as well as the migratory parasitic nematodes Radopholus similis [14] and Bursaphelenchus xylophilus [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

RNA interference in Pratylenchus coffeae: Knock down of Pc-pat-10 and Pc-unc-87 impedes migration

Joseph

Gheysen

Subramaniam

2012

Molecular and Biochemical Parasitology

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Short interfering RNA-mediated knockdown of drosha and pasha in undifferentiated Meloidogyne incognita eggs leads to irregular growth and embryonic lethality

Cited by 32 publications

References 24 publications

Identification of Novel Target Genes for Safer and More Specific Control of Root-Knot Nematodes from a Pan-Genome Mining

Identification of Novel Target Genes for Safer and More Specific Control of Root-Knot Nematodes from a Pan-Genome Mining

Neurobiology of plant parasitic nematodes

RNA interference in Pratylenchus coffeae: Knock down of Pc-pat-10 and Pc-unc-87 impedes migration

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