Short intervals between ultrasonographically guided follicle aspiration improve oocyte quality but do not prevent establishment of dominant follicles in the Gir breed (Bos indicus) of cattle

Viana, J. H. M.; Camargo, L. S. A.; Ferreira, Ademir de Moraes; de, Wanderlei Ferreira; Fernandes, C. A. C.; Marques, A.P.

doi:10.1016/j.anireprosci.2003.12.002

Cited by 51 publications

(49 citation statements)

References 46 publications

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“…Follicles of 3–8 mm were aspirated and cumulus-oocyte complexes (COCs) of grades I and II [25] were selected, and washed twice in medium TALP-HEPES [26]. Groups of 30–40 COCs were then placed into four-well plates (NUNC, Roskilde, Denmark) for in vitro maturation (IVM).…”

Section: Methodsmentioning

confidence: 99%

Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos

Batista

Raposo

Campos-Júnior

et al. 2014

J Animal Sci Biotechnol

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BackgroundDue to high neutral lipids accumulation in the cytoplasm, in vitro-produced embryos from Bos primigenius indicus and their crosses are more sensitive to chilling and cryopreservation than those from Bos primigenius taurus. The objective of the present study was to evaluate the effects of trans-10, cis-12 conjugated linoleic acid (CLA) on the development and cryotolerance of crossbred Bos primigenius taurus x Bos primigenius indicus embryos produced in vitro, and cultured in the presence of fetal calf serum. Bovine zygotes (n = 1,692) were randomly assigned to one of the following treatment groups: 1) Control, zygotes cultured in Charles Rosenkrans 2 amino acid (CR2aa) medium (n = 815) or 2) CLA, zygotes cultured in CR2aa medium supplemented with 100 μmol/L of trans-10, cis-12 CLA (n = 877). Embryo development (cleavage and blastocyst rates evaluated at days 3 and 8 of culture, respectively), lipid content at morula stage (day 5) and blastocyst cryotolerance (re-expansion and hatching rates, evaluated 24 and 72 h post-thawing, respectively) were compared between groups. Additionally, selected mRNA transcripts were measured by Real–Time PCR in blastocyst stage.ResultsThe CLA treatment had no effect on cleavage and blastocyst rates, or on mRNA levels for genes related to cellular stress and apoptosis. On the other hand, abundance of mRNA for the 1-acylglycerol-3-phosphate 0-acyltransferase-encoding gene (AGPAT), which is involved in triglycerides synthesis, and consequently neutral lipid content, were reduced by CLA treatment. A significant increase was observed in the re-expansion rate of embryos cultured with trans-10, cis-12 CLA when compared to control (56.3 vs. 34.4%, respectively, P = 0.002). However, this difference was not observed in the hatching rate (16.5 vs. 14.0%, respectively, P = 0.62).ConclusionsThe supplementation with trans-10, cis-12 CLA isomer in culture medium reduced the lipid content of in vitro produced bovine embryos by reducing the gene expression of 1-acylglycerol 3-phosphate 0-acyltransferase (AGPAT) enzyme. However, a possible improvement in embryo cryotolerance in response to CLA, as suggested by increased blastocyst re-expansion rate, was not confirmed by hatching rates.

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Section: Methodsmentioning

confidence: 99%

Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos

Batista

Raposo

Campos-Júnior

et al. 2014

J Animal Sci Biotechnol

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“…OTU devices can be used safely in calves (52, 84) and the general consensus is that neither well-being nor health of bovine donors subjected to OPU is significantly affected (85)(86)(87). Although application of OTU for the aspiration of ovarian follicles can induce changes in reproductive hormone profiles (88)(89)(90)(91), follicular growth (92), and alter the length of the estrous cycle (93), the reproductive performance after termination of follicular aspirations is usually unaffected (3,76,94,95). Nevertheless, there is evidence indicating the formation of ''residual follicles'' (i.e., follicles that refilled with fluid after follicular aspiration) following OPU, which could result in the formation of follicular cysts or impaired follicular growth (96).…”

Section: Relevance Of Using Otu As Research Tool In Bovine Reproductionmentioning

confidence: 98%

Biomedical Applications of Ovarian Transvaginal Ultrasonography in Cattle

Velazquez

Kues

Niemann

2014

Animal Biotechnology

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Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structures is easily accomplished with bovine OTU techniques providing a promising system for in vivo delivery of transgenes directly into the ovary. The current review summarizes existing bovine OTU models and provides prospective applications of bovine OTU to undertake research in reproductive topics of biomedical relevance, with special emphasis on the development of in vivo gene transfer strategies.

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“…Recovered cumulus oocyte complexes were transferred to 60x15 mm culture dishes containing DPBS medium, where they were counted and sorted based on their morphological appearance, using a stereoscopic microscope with 40 X magnification, as described by Viana et al (2004). After classification, the oocytes were transferred to 1.2 mL cryotubes containing TCM-199 and were transported in a portable incubator, model INC2040-R1 (Bioceler, Belo Horizonte, MG, Brazil), at 37.5°C, to the IVP laboratory.…”

Section: Methodsmentioning

confidence: 99%

Hormonal protocols for in vitro production of Zebu and taurine embryos

Fernandes

Miyauchi²,

Figueiredo

et al. 2014

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound-guided follicular aspirations (n=237) were performed without pre-treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of oocytes to embryos in these treatments. The hot or the cold season did not affect the B. indicus outcomes, whereas, in B. taurus, both oocyte recovery and embryo production were higher in the cold season. Follicular wave synchronization improves ovum pick-up and in vitro production of embryos in both cattle subspecies evaluated.Index terms: Bos indicus, Bos taurus, artificial insemination, follicle growth induction, follicular wave synchronization, FSH stimulation. Protocolos hormonais para produção in vitro de embriões de zebuínos e taurinosResumo -O objetivo deste trabalho foi avaliar os efeitos de protocolos hormonais de sincronização, associados ou não com o estímulo ao desenvolvimento folicular, na recuperação de oócitos e na produção in vitro de embriões de Bos indicus e B. taurus, em diferentes estações. As aspirações foliculares guiadas por ultrassom (n=237) foram realizadas sem pré-tratamento (G1, grupo controle) e após sincronização da onda folicular (G2), ou após sincronização da onda folicular e estímulo do desenvolvimento folicular (G3). Bos indicus produziu mais oócitos e embriões que B. taurus (18,7±0,9 vs. 11,9±0,6 oócitos e 4,8±0,3 vs. 2,1±0,2 embriões). Em média, as produções de oócitos e embriões foram maiores em G3 do que em G2, e ambas foram maiores que em G1, o que levou a uma maior conversão de oócitos em embriões nesses tratamentos. A estação quente ou fria não afetou os resultados de B. indicus, enquanto, em B. taurus, tanto a recuperação de oócitos quanto a produção de embriões foram maiores na estação fria. A sincronização da onda folicular melhora a aspiração folicular e a produção in vitro de embriões, em ambas as subespécies de bovinos avaliadas.Termos para indexação: Bos indicus, Bos taurus, inseminação artificial, indução de crescimento folicular, sincronização da onda folicular, estimulação com FSH.

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Short intervals between ultrasonographically guided follicle aspiration improve oocyte quality but do not prevent establishment of dominant follicles in the Gir breed (Bos indicus) of cattle

Cited by 51 publications

References 46 publications

Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos

Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos

Biomedical Applications of Ovarian Transvaginal Ultrasonography in Cattle

Hormonal protocols for in vitro production of Zebu and taurine embryos

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