Short Report: Detection of 72–75-kD and 123-kD Fractions of Leishmania Antigen in Urine of Patients with Visceral Leishmaniasis

Colmenares, Marı́a; Portús, Montserrat; Riera, Cristina; Gállego, Montserrat; Aisa, M. J.; Torras, S; Muñoz, Carlos Andrés García

doi:10.4269/ajtmh.1995.52.427

Cited by 40 publications

(34 citation statements)

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“…Therefore, we cannot discard that some of the bands found in our electrophoretic study can be an antigenic protein of the parasite. However, none of the two antigenic proteins described by De Colmenares et al [6] seems to be equivalent to the bands detected in this study. First, we did not detect any band with a molecular weight around 123 kDa that could be equivalent to the higher molecular weight band previously published [6].…”

Section: Discussioncontrasting

confidence: 80%

“…However, none of the two antigenic proteins described by De Colmenares et al [6] seems to be equivalent to the bands detected in this study. First, we did not detect any band with a molecular weight around 123 kDa that could be equivalent to the higher molecular weight band previously published [6]. Second, the band found in our study in the range of 70-80 kDa could be equivalent to the band with a lower molecular weight described by De Colmenares et al of 72-75 kDa.…”

Section: Discussioncontrasting

confidence: 80%

“…In a previous study performed on the urine of people suffering from visceral leishmaniasis by De Colmenares et al [6], two fractions of Leishmania antigens of 72-75 kDa and 123 kDa were detected by immunoblot analysis. In our study, we did not develop immunoblotting studies using specific antibodies to Leishmania proteins.…”

Section: Discussionmentioning

confidence: 99%

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SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

et al. 2003

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-Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The results were compared to renal function laboratory tests and to those from a histopathological study of the kidneys from sick animals that died naturally or were euthanized. Five different bands with molecular weights ranging from 10 to 110 kDa were obtained from the electrophoresis of the urine of healthy dogs. 33.5% of total proteins corresponded to low molecular weight proteins and the other proteins had middle and high molecular weights. However, in the group with leishmaniasis, a maximum of 11 different bands with molecular weights ranging from 10 kDa to 150 kDa were displayed in the electrophoresis of the urine. The urine electrophoretic pattern in the sick dogs was classified as mixed (proteins with high and low molecular weights) because low molecular weight proteins made up 57.9% and the rest of the proteins had middle and high molecular weights. In Western blot, none of the healthy dogs showed excretion of IgG and/or IgA, whereas IgG and IgA were detected in the Western blot of urine of 68% and 55% respectively of dogs with leishmaniasis. The results obtained in the leishmaniasis group agreed with glomerular and tubular damage, which were confirmed by the histopathological findings. leishmaniasis / proteinuria / dog / SDS-PAGE / Western blot

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Section: Discussioncontrasting

confidence: 80%

Section: Discussioncontrasting

confidence: 80%

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SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

et al. 2003

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“…For this the simultaneous occurrence of two heat shock proteins Hsp70 and Hsp83 (Skeiky et al 1997). In another study, two polypeptide fractions of 72-75 kDa were detected in the urine of 14 of 15 patients with visceral leishmaniasis (VL) and another fraction of 123 kDa was found in 10 of the 15 patients with 96 % sensitivity and 100 % specificity (De Colmenares et al 1995).…”

Section: Discussionmentioning

confidence: 98%

ELISA and western blotting for the detection of Hsp70 and Hsp83 antigens of Leishmania donovani

Kaur

2012

J Parasit Dis

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“…g) Detection of72-75 kDa and 123 kDa fractions of Leishmania antigen in urine may be used to diagnose kala azar [9]. These tests are particularly useful for diagnosis in subclinical infections and in population screening as they are more sensitive and specific than the conventional aldehyde test.…”

Section: Diagnosismentioning

confidence: 99%

Management of Kala Azar – An Update

Kalra

Bahl

Sanchetee

et al. 1996

Medical Journal Armed Forces India

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Kala azar continues to be a medical problem in India and with the increase in incidence of IIIV Infectiun it is likely that kala azar will be encountered more frequently and in its atypical forms. To.aid diagnosis, several immunological tests are now available and they are more sensitive and specific than the aldehyde test. Like many other diseases today, the treatment sf kala azar is hampered b)' drug resistance, Newer drugs are available and so are new delivery systems. Kala azar develops frequently in the I1IV infected person before development of AIDS. The presentation is atypical and leishmanial species other than L. donovani may also he the infecting agents. A combination of sandfly control, detection and treatment of patients and prevention of drug resistance continues to the ideal approach for the control of the disease. MJAFI 1996; 52: 189-192

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Short Report: Detection of 72–75-kD and 123-kD Fractions of Leishmania Antigen in Urine of Patients with Visceral Leishmaniasis

Cited by 40 publications

References 1 publication

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

SDS-PAGE and Western blot of urinary proteins in dogs with leishmaniasis

ELISA and western blotting for the detection of Hsp70 and Hsp83 antigens of Leishmania donovani

Management of Kala Azar – An Update

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