Short report: occurrence of Leishmania donovani DNA in donated blood from seroreactive Brazilian blood donors.

Otero, Alejandra; Silva, V. O. da; Luz, Kléber Giovanni; Palatnik, Marcos; Pirmez, Claude; Fernandes, Octávio; Palatnik-de-Sousa, Clarisa B.

doi:10.4269/ajtmh.2000.62.128

Cited by 64 publications

(50 citation statements)

References 13 publications

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“…Cutaneous leishmaniasis is highest in the Middle East, Syria, Brazil, and Peru (64), but imported cutaneous leishmaniasis is becoming problematic in the United States, particularly in military personnel (31,57). Leishmaniasis in the blood supply is becoming a concern (10,48), and an outbreak of Leishmania (L.) infantum infection affecting foxhounds has introduced a possible reservoir into the United States (52). As such, leishmaniasis is emerging as a disease of concern with both human and veterinary importance.…”

Section: Discussionmentioning

confidence: 99%

Serial Quantitative PCR Assay for Detection, Species Discrimination, and Quantification of Leishmania spp. in Human Samples

et al. 2011

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The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species in clinical specimens is established. We developed a serial qPCR strategy to identify and rapidly differentiate Leishmania species and quantify parasites in clinical or environmental specimens. SYBR green qPCR is mainly employed, with corresponding TaqMan assays for validation. The screening primers recognize kinetoplast minicircle DNA of all Leishmania species. Species identification employs further qPCR set(s) individualized for geographic regions, combining species-discriminating probes with melt curve analysis. The assay was sufficient to detect Leishmania parasites, make species determinations, and quantify Leishmania spp. in sera, cutaneous biopsy specimens, or cultured isolates from subjects from Bangladesh or Brazil with different forms of leishmaniasis. The multicopy kinetoplast DNA (kDNA) probes were the most sensitive and useful for quantification based on promastigote standard curves. To test their validity for quantification, kDNA copy numbers were compared between Leishmania species, isolates, and life stages using qPCR. Maxicircle and minicircle copy numbers differed up to 6-fold between Leishmania species, but the differences were smaller between strains of the same species. Amastigote and promastigote leishmania life stages retained similar numbers of kDNA maxi-or minicircles. Thus, serial qPCR is useful for leishmania detection and species determination and for absolute quantification when compared to a standard curve from the same Leishmania species.

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Section: Discussionmentioning

confidence: 99%

Serial Quantitative PCR Assay for Detection, Species Discrimination, and Quantification of Leishmania spp. in Human Samples

et al. 2011

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“…PCR protocols to detect Leishmania DNA in VL diagnosis have used a variety of samples, including spleen, lymph node, and bone marrow aspirates, whole blood, and buffy coat [92,[95][96][97][98][99][100][101].…”

Section: Molecular Methods: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Diagnosis of leishmaniasis

Elmahallawy

Martínez

Rodríguez‐Grangér

et al. 2014

J Infect Dev Ctries

154

119

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Leishmaniasis is a clinically heterogeneous syndrome caused by intracellular protozoan parasites of the genus Leishmania. The clinical spectrum of leishmaniasis encompasses subclinical ( not apparent), localized (skin lesion), and disseminated (cutaneous, mucocutaneous, and visceral) infection. This spectrum of manifestations depends on the immune status of the host, on the parasite, and on immunoinflammatory responses. Visceral leishmaniasis causes high morbidity and mortality in the developing world. Reliable laboratory methods become mandatory for accurate diagnosis, especially in immunocompromised patients such as those infected with HIV. In this article, we review the current state of the diagnostic tools for leishmaniasis, especially the serological test.

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“…This mode of transmission would be limited to organs that form part of the reticuloendothelial system, such as the liver. Second, although anecdotal, cases of transfusion-associated VL have been reported in Great Britain, France, Belgium, and India (275). Routine serology testing for Leishmania spp.…”

Section: Infections Caused By Leishmania Sppmentioning

confidence: 99%

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

et al. 2008

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SUMMARY In recent years, the increasing number of donors from different regions of the world is providing a new challenge for the management and selection of suitable donors. This is a worldwide problem in most countries with transplantation programs, especially due to the increase in immigration and international travel. This paper elaborates recommendations regarding the selection criteria for donors from foreign countries who could potentially transmit tropical or geographically restricted infections to solid-organ transplant recipients. For this purpose, an extensive review of the medical literature focusing on viral, fungal, and parasitic infections that could be transmitted during transplantation from donors who have lived or traveled in countries where these infections are endemic has been performed, with special emphasis on tropical and imported infections. The review also includes cases described in the literature as well as risks of transmission during transplantation, microbiological tests available, and recommendations for each infection. A table listing different infectious agents with their geographic distributions and specific recommendations is included.

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Short report: occurrence of Leishmania donovani DNA in donated blood from seroreactive Brazilian blood donors.

Cited by 64 publications

References 13 publications

Serial Quantitative PCR Assay for Detection, Species Discrimination, and Quantification of Leishmania spp. in Human Samples

Serial Quantitative PCR Assay for Detection, Species Discrimination, and Quantification of Leishmania spp. in Human Samples

Diagnosis of leishmaniasis

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

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