Short Session High Intensity Interval Training and Treadmill Assessment in Aged Mice

Seldeen, Kenneth L.; Redae, Yonas; Thiyagarajan, Ramkumar; Berman, Reem Nagi; Leiker, Merced M; Troen, Bruce R.

doi:10.3791/59138

Cited by 11 publications

(12 citation statements)

References 40 publications

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“…In contrast, resistance training (e.g., weight lifting) in humans primarily improves strength and muscle mass, with less improvement in aerobic capacity than endurance training (Phillips SM, 2007). Similar results have been observed in mouse models of exercise (Graber et al, 2015;Graber et al, 2019a;Seldeen et al, 2019).…”

Section: Introductionsupporting

confidence: 88%

“…Rodent models demonstrate similar sarcopenic outcomes to those mentioned above in humans (Parks et al, 2012;Graber et al, 2013;Liu et al, 2014;Graber et al, 2021). Exercise, however, can help mitigate frailty and preserve physical function in both humans and mice (Koster et al, 2012;Reeves et al, 2004;Graber et al, 2015;Medeiros et al, 2008;Seldeen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Endurance exercise preserves physical function in adult and older male C57BL/6 mice: high intensity interval training (HIIT) versus voluntary wheel running (VWR)

Pajski,

Byrd,

Nandigama

et al. 2024

Front. Aging

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Exercise has been shown to improve physical function, mitigate aspects of chronic disease and to potentially alter the trajectory of age-related onset of frailty and sarcopenia. Reliable and valid preclinical models are necessary to elucidate the underlying mechanisms at the intersection of age, exercise, and functional decline. The purpose of this study was to compare, head to head, the effects of two common pre-clinical models of endurance exercise: high intensity interval training (HIIT) and voluntary wheel running (VWR). The hypothesis was that a prescribed and regimented exercise program, HIIT, would prove to be a superior training method to unregulated voluntary exercise, VWR. To investigate this hypothesis, we evaluated adult (n = 24, designated 10 m, aged 6 months at the beginning of the study, 10 months at its completion) and older adult (n = 18, designated 26 m, aging from 22 months to 26 months over the course of the study) C57BL/6 male mice. These mice were randomly assigned (with selection criteria) to a 13-week program of voluntary wheel running (VWR), high intensity interval training (HIIT), or sedentary control (SED). The functional aptitude of each mouse was determined pre- and post-training using our composite CFAB (comprehensive functional assessment battery) scoring system consisting of voluntary wheel running (volitional exercise and activity rate), treadmill (endurance), rotarod (overall motor function), grip meter (forelimb strength), and inverted cling (whole body strength/endurance). To measure sarcopenia, we tracked body mass, body composition (with EchoMRI), plantar flexor torque (in 10 m), and measured muscle wet mass post-training. Overall, adult CFAB scores decreased while body mass and percent body fat increased as they matured; however, exercise significantly mitigated the changes (p < 0.05) compared to SED. Older adults demonstrated preservation of function (CFAB) and reduced body fat (p < 0.05) compared to SED. To conclude, both types of exercise maintained physical function equally in older mice.

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Section: Introductionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Endurance exercise preserves physical function in adult and older male C57BL/6 mice: high intensity interval training (HIIT) versus voluntary wheel running (VWR)

Pajski,

Byrd,

Nandigama

et al. 2024

Front. Aging

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“…Treadmill assessment was performed on a mouse treadmill (Columbus Instruments) set with no inclination (flat—0°), as we described previously [ 21 ]. Briefly, at each timepoint, an endurance assessment was performed whereby the treadmill belt accelerated from 5 to 35 m/min over 60 min and the time on the belt was recorded at exhaustion for each mouse, defined as 10 visits to a shock pad or 20 total shocks (shocks at 54 V, 0.72 mA).…”

Section: Methodsmentioning

confidence: 99%

Vitamin D Insufficiency Reduces Grip Strength, Grip Endurance and Increases Frailty in Aged C57Bl/6J Mice

et al. 2020

Self Cite

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Low 25-OH serum vitamin D (VitD) is pervasive in older adults and linked to functional decline and progression of frailty. We have previously shown that chronic VitD insufficiency in “middle-aged” mice results in impaired anaerobic exercise capacity, decreased lean mass, and increased adiposity. Here, we examine if VitD insufficiency results in similar deficits and greater frailty progression in old-aged (24 to 28 months of age) mice. Similar to what we report in younger mice, older mice exhibit a rapid and sustained response in serum 25-OH VitD levels to differential supplementation, including insufficient (125 IU/kg chow), sufficient (1000 IU/kg chow), and hypersufficient (8000 IU/kg chow) groups. During the 4-month time course, mice were assessed for body composition (DEXA), physical performance, and frailty using a Fried physical phenotype-based assessment tool. The 125 IU mice exhibited worse grip strength (p = 0.002) and inverted grip hang time (p = 0.003) at endpoint and the 8000 IU mice transiently displayed greater rotarod performance after 3 months (p = 0.012), yet other aspects including treadmill performance and gait speed were unaffected. However, 125 and 1000 IU mice exhibited greater frailty compared to baseline (p = 0.001 and p = 0.038, respectively), whereas 8000 IU mice did not (p = 0.341). These data indicate targeting higher serum 25-OH vitamin D levels may attenuate frailty progression during aging.

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“…We do know from this study that the in the 10m CON we saw a large degree of degradation in functional capacity that was offset by exercise, and in the past we and others have shown that various forms of exercise do either improve function or mitigate the degree of functional loss versus sedentary controls in older adult mice (Graber, 2015;Graber, 2019a;Seldeen 2018;Seldeen 2019). Secondly, we may have erred on the side of caution in advancing the HIIT protocol intensity.…”

Section: Caveatsmentioning

confidence: 61%

Endurance Exercise to Improve Physical Function in Adult and Older Mice: High Intensity Interval Training (HIIT) versus Voluntary Wheel Running (VWR)

Byrd

Nandigama

et al. 2021

Preprint

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With age comes a gradual decline in physical function and exercise capacity, concurrent with a progressive propensity for development of sarcopenia /(age-related loss of muscle mass and strength) and frailty (inability of body to thrive and maintain homeostasis). Prior research has demonstrated that exercise, while not a cure, can help mitigate sarcopenia/frailty and restore functional capacity. Reliable, validated, pre-clinical models are necessary to elucidate the underlying molecular mechanisms at the intersection of age, exercise, and functional decline. In this study, we hypothesized that endurance exercise programs mimicking typical human exercise protocols would improve physical function in both adult and older adult mice. Furthermore, our secondary hypothesis was that older mice would receive less benefit from a similar volume of exercise than adult mice. To test these hypotheses, we randomly assigned (with some selection criteria) male C57BL/6 mice either at adult ages during the study (n=24, designated 10m, aged 6 months to 10 months) or at older adult ages (n=18, designated 26m, aged 22 months to 26 months) to a 13-week program of voluntary wheel running (VWR, group termed RUN) or high intensity interval training (HIIT), with an additional 10m sedentary control (CONT). The functional aptitude of each mouse was determined pre- and post-training using our composite CFAB (comprehensive functional assessment battery) scoring system consisting of voluntary wheel running (volitional exercise and activity rate), treadmill (endurance), rotarod (overall motor function), grip meter (forelimb strength), and inverted cling (whole body strength/endurance). To measure sarcopenia, we tracked body mass and body composition changes with EchoMRI, and measured muscle wet mass post-training.

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Short Session High Intensity Interval Training and Treadmill Assessment in Aged Mice

Cited by 11 publications

References 40 publications

Endurance exercise preserves physical function in adult and older male C57BL/6 mice: high intensity interval training (HIIT) versus voluntary wheel running (VWR)

Endurance exercise preserves physical function in adult and older male C57BL/6 mice: high intensity interval training (HIIT) versus voluntary wheel running (VWR)

Vitamin D Insufficiency Reduces Grip Strength, Grip Endurance and Increases Frailty in Aged C57Bl/6J Mice

Endurance Exercise to Improve Physical Function in Adult and Older Mice: High Intensity Interval Training (HIIT) versus Voluntary Wheel Running (VWR)

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