Short-term antimicrobial properties of mineral trioxide aggregate with incorporated silver-zeolite

Abstract

The purpose of this in vitro study was to determine whether adding silver-zeolite (SZ) to mineral trioxide aggregate (MTA) would enhance the antimicrobial activity of MTA against Staphylococcus aureus (ATCC #25923), Enterococcus faecalis (ATCC #29212), Escherichia coli (ATCC#25922), Pseudomonas aeruginosa (ATCC #27853), Candida albicans (ATCC #90028), Porphyromonas gingivalis (ATCC #33277), Actinomyces israelii (ATCC #12102), and Prevotella intermedia (ATCC# 15032). SZ was added at 0.2% and 2% mass fraction concentration to MTA powder. The control group was MTA powder with no SZ. The antimicrobial effect test was accomplished by placing freshly mixed MTA specimens on agar plates inoculated with microorganisms and comparing the zones of inhibition at 24, 48, and 72 h. The amounts of silver ion release from MTA specimens were measured with atomic absorption spectrophotometry at 10-min, 24-, 48-, and 72-h periods. The pH of MTA specimens was measured with a pH meter at 10-min, 24-, 48-, and 72-h periods. MTA with 2% and 0.2% SZ specimens showed inhibitory effects on some microorganisms at all time periods, whereas no antimicrobial activity showed for P. intermedia and A. israelii. MTA without SZ inhibited C. albicans, E. Coli, and P. intermedia. The highest silver release was detected in 2% SZ MTA at 24 h. The incorporation of SZ may enhance the antimicrobial activity of MTA.

Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers

“…The antimicrobial effect of IRM could be explained by the antibacterial ability of eugenol released from IRM by progressive hydrolysis [38]. No antibacterial effect of MTA against P. ginigivalis was observed in the current study, which is consistent with previous studies [30,39]. All tested cements caused significant but limited reduction on E. faecalis growth ranging from 2-15% compared to control.…”

Radiopacity, pH and antimicrobial activity of Portland cement associated with micro- and nanoparticles of zirconium oxide and niobium oxide

“…The antimicrobial activity evaluation showed similar results for all the microorganisms evaluated. The addition of substances to MTA aiming to improve its antimicrobial effect has been studied [27][28][29] . Odabas et al 29) demonstrated that silver addition to MTA improves its antimicrobial property.…”

“…The addition of substances to MTA aiming to improve its antimicrobial effect has been studied [27][28][29] . Odabas et al 29) demonstrated that silver addition to MTA improves its antimicrobial property. Saatchi 27) et al observed that the inclusion of potassium iodide does not alter the antimicrobial effect of MTA.…”

The effects of incorporation of silver-zeolite on selected properties of mineral trioxide aggregate

“…It has been showed that incorporating SZ into MTA was sufficient for enhancing its antimicrobial activity 16) . However, there was no study to evaluate the influence of SZ on chemical and physical properties of MTA.…”

“…An in vitro previous study showed favourable antimicrobial effects of MTA containing SZ on several microorganisms. It also has been clarified that incorporated SZ increased the antimicrobial properties of MTA 16) . However it is very important that physicochemical properties of MTA containing SZ be considered in relation to their use because such filling material should present biocompatibility, adequate setting time, insolubility and antimicrobial activity 17) .…”