Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

Sota, Valbona; Benelli, Carla; Myrselaj, Matilda; Kongjika, Efigjeni; Gruda, Nazim S.

doi:10.3390/horticulturae9050559

Cited by 4 publications

(1 citation statement)

References 63 publications

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“…The walnut tree is considered one of the most recalcitrant species, thereby presenting difficulties regarding its proliferation rate. Therefore, for proliferation in in vitro conditions, it is necessary to define the optimal environmental conditions for its proliferation (Sota et al, 2023). In the commercial tissue culture of walnut, the number of lateral shoots grown from one explant is very important.…”

Section: Discussionmentioning

confidence: 99%

Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra

Saeedi,

Vahdati,

Sarikhani

et al. 2023

Front. Plant Sci.

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Light plays a crucial role in photosynthesis, which is an essential process for plantlets produced during in vitro tissue culture practices and ex vitro acclimatization. LED lights are an appropriate technology for in vitro lighting but their effect on propagation and photosynthesis under in vitro condition is not well understood. This study aimed to investigate the impact of different light spectra on growth, photosynthetic functionality, and stomatal characteristics of micropropagated shoots of Persian walnut (cv. Chandler). Tissue-cultured walnut nodal shoots were grown under different light qualities including white, blue, red, far-red, green, combination of red and blue (70:30), combination of red and far-red (70:30), and fluorescent light as the control. Results showed that the best growth and vegetative characteristics of in vitro explants of Persian walnut were achieved under combination of red and blue light. The biggest size of stomata was detected under white and blue lights. Red light stimulated stomatal closure, while stomatal opening was induced under blue and white lights. Although the red and far-red light spectra resulted in the formation of elongated explants with more lateral shoots and anthocyanin content, they significantly reduced the photosynthetic functionality. Highest soluble carbohydrate content and maximum quantum yield of photosystem II were detected in explants grown under blue and white light spectra. In conclusion, growing walnut explants under combination of red and blue lights leads to better growth, photosynthesis functionality, and the emergence of functional stomata in in vitro explants of Persian walnuts.

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Section: Discussionmentioning

confidence: 99%

Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra

Saeedi,

Vahdati,

Sarikhani

et al. 2023

Front. Plant Sci.

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The Role of Synthetic Seeds in Germplasm Conservation of Forest Tree Species

Tailor,

Joshi

2024

Biotechnological Approaches for Sustaining Forest Trees and Their Products

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Blackberry synthetic seeds storage: effects of temperature, time, and sowing substrate

Regni,

Micheli,

Pino

et al. 2024

Plant Cell Tiss Organ Cult

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In vitro propagation, is becoming the predominant method for blackberry propagation due to its advantages compared to agamic traditional propagation methods. Synthetic seed technology represents a promising approach to further enhance the productivity of in vitro propagation facilitating the exchange of plant materials among laboratories and contributing to germplasm conservation efforts. This study aimed to establish an optimal protocol for the storage and sowing of synthetic blackberry seeds obtained through the encapsulation of clump bases. The synthetic seeds were sown without storage (Control) and after storage periods of 30, 60, and 120 days at 4 °C and 25 °C in the dark, in three different substrates (agarised, perlite, and potting). After forty-five days from sowing viability, regeneration rate, shoot and root numbers and lengths, as well as fresh and dry weights of the plantlets, were assessed. Results indicated that agarised substrate consistently exhibited favourable outcomes, with sustained regeneration rates and robust plantlet development even after prolonged storage at 4 °C. Synthetic seeds sown in perlite and potting substrates demonstrated enhanced regeneration rates following storage at 4 °C for 60 and 120 days. On the contrary, storage at 25 °C resulted in a notable decline in regeneration rate, highlighting its inadequacy for blackberry synthetic seed conservation purposes. These findings underscore the importance of sowing substrate selection and storage temperature in optimizing the storage and sowing protocols for synthetic blackberry seeds.

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Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

Cited by 4 publications

References 63 publications

Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra

Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra

The Role of Synthetic Seeds in Germplasm Conservation of Forest Tree Species

Blackberry synthetic seeds storage: effects of temperature, time, and sowing substrate

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