Short-term dietary adjustment with a hydrolyzed casein–based diet postpones diabetes development in the diabetes-prone BB rat

Visser, J.H.M.; Brugman, Sylvia; Klatter, Flip A.; Vis, L; Groen, Herman; Strubbe, J.H.; Rozing, Jan

doi:10.1053/meta.2003.50052

Cited by 18 publications

(25 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The degree of insulitis was calculated as described by Visser et al (4,15). Upon autopsy, the pancreas was removed, cleaned of fat and lymph nodes, fixed in Bouin's solution and processed for histological analysis.…”

Section: Pancreas Histologymentioning

confidence: 99%

Long-term prophylactic insulin treatment can prevent spontaneous diabetes and thyroiditis development in the diabetes-prone bio-breeding rat, while short-term treatment is ineffective

Visser

Klatter

Vis

et al. 2003

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: Prophylactic insulin treatment has been demonstrated to reduce diabetes development in the diabetes-prone bio-breeding (DP-BB) rat. These prophylactic insulin treatments were given from 50 to 150 days of age. However, several data indicate that the diabetogenic process in DP-BB rats starts well before day 50. Design and methods: DP-BB rats were given bovine insulin pellets from 21 to 60 days of age, from 21 to 100 days of age and from 60 to 100 days of age. At 160 days of age a glucose tolerance test was performed to establish b-cell function and pancreata collected for histological analysis. Results: Prophylactic insulin treatment from 21 to 100 days of age gave a 42% reduction of diabetes incidence. The other treatment protocols had no effect. Non-diabetic rats treated with insulin from day 21 to 100 showed normal glucose tolerance and no sign of insulitis at 160 days of age. Non-diabetic rats of the control group and the other treatment groups showed normal glucose tolerance, but a slight increase of insulitis. Interestingly, the 21-100 day treated rats showed reduced serum levels of anti-colloid antibodies as compared with the control group. Conclusions: These results show that short-term prophylactic insulin treatment cannot prevent diabetes and thyroiditis development in DP-BB rats. The prophylactic treatment must start well before 60 days of age and be prolonged into the phase when the rats normally become diabetic to reduce diabetes incidence. These findings imply that in the human situation prophylactic insulin treatment must be prolonged over the normal range of diabetes onset.

show abstract

Section: Pancreas Histologymentioning

confidence: 99%

Long-term prophylactic insulin treatment can prevent spontaneous diabetes and thyroiditis development in the diabetes-prone bio-breeding rat, while short-term treatment is ineffective

Visser

Klatter

Vis

et al. 2003

European Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Several other research groups have reported that an HC-diet has a protective effect on the development of diabetes in the BB-DP [6,7,8]. The protective effect of an HC-diet against diabetes could be the result of an actively induced protective mechanism or of nonexposure to diabetogenic substances present in plantbased rodent diets [6,7].…”

Section: Discussionmentioning

confidence: 99%

“…The protective effect of an HC-diet against diabetes could be the result of an actively induced protective mechanism or of nonexposure to diabetogenic substances present in plantbased rodent diets [6,7].…”

Section: Discussionmentioning

confidence: 99%

“…Sections of 7 µm were stained with haematoxylin/eosin to evaluate insulitis. The degree of islet infiltration was measured using a Zeiss microscope and was rated as previously described [6] with the addition of score 5, which was developed in our lab to control for completely destroyed islets in severe insulitis. Per pancreas section, an average histological insulitis score was calculated by adding up the score of each islet and dividing it by the total number of islets counted.…”

Section: Methodsmentioning

confidence: 99%

“…In this study we investigated whether oral administration of DiaPep277 (a synthetic peptide analog of p277) affected the development of diabetes in neonatal BioBreeding diabetes-prone (BB-DP) rats, and whether this could potentiate the effect of a protective hydrolysed casein (HC) diet [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Neonatal oral administration of DiaPep277, combined with hydrolysed casein diet, protects against Type 1 diabetes in BB-DP rats. An experimental study

Brugman

Klatter

Visser³

et al. 2004

Diabetologia

View full text Add to dashboard Cite

Aims/hypothesis. Environmental factors such as diet and bacterial antigens play an important role in the onset of Type 1 diabetes. Different self-antigens are suggested to play a role in the development of diabetes. Antibodies against the 60-kDa heat shock protein 60, which have a high homology to bacterial heat shock protein 65, have been found in the circulation at the onset of diabetes in humans and in pre-diabetic NOD-mice. One of the immunodominant epitopes in autoimmune diabetes is p277, a specific peptide of human heat shock protein 60 corresponding to positions 437-460. In this study we investigated whether neonatal oral administration of DiaPep277 (a synthetic peptide analogue of p277) affected the development of diabetes in the BioBreeding-Diabetes Prone (BB-DP) rat, and whether this could potentiate the effect of a protective hydrolysed casein-diet.Methods. BB-DP rats were orally inoculated once per day with placebo or DiaPep277 at days 4, 5, 6 and 7 of life. At the age of 21 days rats were weaned on to a conventional, cereal-based diet or on to the hydrolysed casein-diet. Results. The development of diabetes in animals receiving DiaPep277 in combination with the hydrolysed casein-diet was delayed by 17 days, and a relative reduction of the incidence by 64% was seen. Nondiabetic animals did not show any sign of insulitis. Conclusions/interpretation. Short-term neonatal feeding with p277 in early life, combined with diet adaptation, appears to provide a procedure to significantly reduce the development of Type 1 diabetes in later life.Keywords BB-DP rat · DiaPep277 · Hydrolysed casein diet · Neonatal · Oral administration · Type 1 diabetes Abbreviations: BB-DP, BioBreeding diabetes-prone · HC, hydrolysed casein · hsp60/65, heat shock protein 60/65 · ICA-69, islet cell antigen 69 · NOD, non-obese diabetic

show abstract

References

2009

Food and Western Disease

View full text Add to dashboard Cite

Short-term dietary adjustment with a hydrolyzed casein–based diet postpones diabetes development in the diabetes-prone BB rat

Cited by 18 publications

References 26 publications

Long-term prophylactic insulin treatment can prevent spontaneous diabetes and thyroiditis development in the diabetes-prone bio-breeding rat, while short-term treatment is ineffective

Long-term prophylactic insulin treatment can prevent spontaneous diabetes and thyroiditis development in the diabetes-prone bio-breeding rat, while short-term treatment is ineffective

Neonatal oral administration of DiaPep277, combined with hydrolysed casein diet, protects against Type 1 diabetes in BB-DP rats. An experimental study

References

Contact Info

Product

Resources

About