Short-Term Effect of Topical Dorzolamide Hydrochloride on Intrastromal Corneal Pressure in Rabbit Corneas In Vivo

Abstract

Measuring ICP is a valid and sensitive method to evaluate in vivo the endothelial function. This method seems to be more sensitive than measuring the central corneal thickness or the corneal deswelling rate in detecting changes in the corneal physiology with the use of topical dorzolamide.

Effect of topical prostaglandin analogues on corneal hysteresis

“…), and these drugs reduce the negative intrastromal corneal pressure created by the endothelial pump function (Teus et al. ).…”

“…Different topical antiglaucoma medications can modify some corneal properties, and these changes may be unrelated to the drug-induced changes in IOP. For instance, topical carbonic anhydrase inhibitors can induce corneal oedema in predisposed eyes (Adamson 1999;Konowal et al 1999), and these drugs reduce the negative intrastromal corneal pressure created by the endothelial pump function (Teus et al 2009).…”

Carbonic Anhydrase Inhibitors in Corneal Endothelial Transport

“…It is also possible that although an increase in corneal thickness is not noted with CAIs in noncompromised human corneas, a more sensitive measurement of changes in intrastromal pressure may reveal subtle differences. 31 Human tissue research is challenging secondary to tissue availability, expense, and quality. However, our data highlight the importance of verifying observations from other species with human tissue experimentation if we are to make correlations and ultimately solve the problems of human eye disease.…”

Cornea and Glaucoma

“…Some investigations have report-ed a slight but significant increase in CCT in eyes treated with brinzolamide, another CAI inhibitor [90]. Another way to measure the endothelial function in vivo is to measure the intrastromal corneal pressure [91,92], formerly known as corneal intrastromal pressure, which has a negative value under physiologic conditions. The amount of negative pressure in the corneal stroma is likely to be correlated with the endothelial function, and topical dorzolamide significantly reduces the negative pressure in the corneal stroma in rabbits [91], suggesting that the drug affects the endothelial function in healthy rabbit corneas.…”

“…Another way to measure the endothelial function in vivo is to measure the intrastromal corneal pressure [91,92], formerly known as corneal intrastromal pressure, which has a negative value under physiologic conditions. The amount of negative pressure in the corneal stroma is likely to be correlated with the endothelial function, and topical dorzolamide significantly reduces the negative pressure in the corneal stroma in rabbits [91], suggesting that the drug affects the endothelial function in healthy rabbit corneas. This finding is consistent with the reported cases of dorzolamide-induced corneal edema in susceptible patients and with the finding that inhibiting CA in the corneal endothelium causes a 50% decrease in the endothelial fluid transport and some corneal swelling [92].…”