Short‐term exposure to synthetic flaxseed lignan LGM2605 alters gut microbiota in mice

Badger, Reagan; Aho, Ken; Serve, Kinta M.

doi:10.1002/mbo3.1185

Cited by 7 publications

(4 citation statements)

References 50 publications

(114 reference statements)

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“…As expected, the unabsorbed lignan was utilized as substrate by enterolactone-producing bacterial species, thus improving the gut microbiota diversity. These results were also observed in normal-fed mice models [44,45] and an in vitro pilot study [46]. Our results suggest that flaxseed lignan can modulate the composition of gut microbiota in the presence of paracetamol-induced liver injury.…”

Section: Discussionsupporting

confidence: 77%

Flaxseed Lignan Alleviates the Paracetamol-Induced Hepatotoxicity Associated with Regulation of Gut Microbiota and Serum Metabolome

Ren,

Xu,

Qiao

et al. 2024

Nutrients

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This study examined the protective effect of flaxseed lignans on liver damage caused by an overdose of paracetamol (PAM). The findings demonstrated that administering 800 mg/kg/d flaxseed lignan prior to PAM significantly decreased the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TBi) levels, while it increased liver superoxide dismutase (SOD) and glutathione (GSH) levels in mice. Flaxseed lignan renovated the gut microbiota dysbiosis induced by PAM by promoting the proliferation of sulfonolipid (SL) producing bacteria such as Alistipes and lignan-deglycosolating bacteria such as Ruminococcus while inhibiting the growth of opportunistic pathogen bacteria such as Acinetobacter and Clostridium. Furthermore, flaxseed lignan modulated the serum metabolomic profile after PAM administration, specifically in the taurine and hypotaurine metabolism, phenylalanine metabolism, and pyrimidine metabolism. The study identified eight potential biomarkers, including enterolactone, cervonyl carnitine, acutilobin, and PC (20:3(5Z, 8Z, 11Z)/20:0). Overall, the results suggest that flaxseed lignan can alleviate PAM-induced hepatotoxicity and may be beneficial in preventing drug-induced microbiome and metabolomic disorders.

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Section: Discussionsupporting

confidence: 77%

Flaxseed Lignan Alleviates the Paracetamol-Induced Hepatotoxicity Associated with Regulation of Gut Microbiota and Serum Metabolome

Ren,

Xu,

Qiao

et al. 2024

Nutrients

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“…Previous studies have shown that consuming FS or isolated FS components can alter the composition and function of the microbiota in humans [ 35 , 71 ] and rodent models [ 36 , 40 , 41 , 72 , 73 ], which may play a role in modulating the response to the LPS challenge. Therefore, we analyzed the fecal microbiota from mice following the three-week dietary intervention, prior to the SAL/LPS challenge, to assess the impact of diet on the baseline microbiota.…”

Section: Resultsmentioning

confidence: 99%

Dietary Flaxseed and Flaxseed Oil Differentially Modulate Aspects of the Microbiota Gut–Brain Axis Following an Acute Lipopolysaccharide Challenge in Male C57Bl/6 Mice

et al. 2023

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The microbiota gut–brain axis (mGBA) is an important contributor to mental health and neurological and mood disorders. Lipopolysaccharides (LPS) are endotoxins that are components of Gram-negative bacteria cell walls and have been widely shown to induce both systemic and neuro-inflammation. Flaxseed (Linum usitatissimum) is an oilseed rich in fibre, n3-poly-unsaturated fatty acid (alpha-linolenic acid (ALA)), and lignan, secoisolariciresinol diglucoside, which all can induce beneficial effects across varying aspects of the mGBA. The objective of this study was to determine the potential for dietary supplementation with flaxseed or flaxseed oil to attenuate LPS-induced inflammation through modulation of the mGBA. In this study, 72 5-week-old male C57Bl/6 mice were fed one of three isocaloric diets for 3 weeks: (1) AIN-93G basal diet (BD), (2) BD + 10% flaxseed (FS), or (3) BD + 4% FS oil (FO). Mice were then injected with LPS (1 mg/kg i.p) or saline (n = 12/group) and samples were collected 24 h post-injection. Dietary supplementation with FS, but not FO, partially attenuated LPS-induced systemic (serum TNF-α and IL-10) and neuro-inflammation (hippocampal and/or medial prefrontal cortex IL-10, TNF-α, IL-1β mRNA expression), but had no effect on sickness and nest-building behaviours. FS-fed mice had enhanced fecal microbial diversity with increased relative abundance of beneficial microbial groups (i.e., Lachnospiraceae, Bifidobacterium, Coriobacteriaceae), reduced Akkermansia muciniphila, and increased production of short-chain fatty acids (SCFAs), which may play a role in its anti-inflammatory response. Overall, this study highlights the potential for flaxseed to attenuate LPS-induced inflammation, in part through modulation of the intestinal microbiota, an effect which may not be solely driven by its ALA-rich oil component.

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“…A few recent studies reported the modulating actions of lignans on gut microbial composition. Bader et al [36] revealed that flaxseed lignan secoisolariciresinol diglucoside altered 3 bacterial phyla and 22 genera after 10-day treatment in eightweek-old female C57BL/6 mice. Taibi et al [37] found that lignan-rich oilseeds could strongly influence the microbiota composition of younger and premenopausal females, and led to different enterolignan production in an in vitro fermentation experiment.…”

Section: Discussionmentioning

confidence: 99%

The Lignan-Rich Fraction from Sambucus williamsii Hance Exerts Bone Protective Effects via Altering Circulating Serotonin and Gut Microbiota in Rats

Xiao

Zhu

et al. 2022

Nutrients

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Our previous study revealed that the bone anabolic effects of the lignan-rich fraction (SWCA) from Sambucus williamsii Hance was involved in modulating the metabolism of tryptophan in vivo and inhibiting serotonin (5-HT) synthesis in vitro. This study aimed to determine how SWCA modulates bone metabolism via serotonin in vivo. The effects of SWCA were evaluated by using 4-month-old Sprague-Dawley (SD) ovariectomized rats. The serum levels of 5-HT and kynurenine, the protein expressions of tryptophan hydroxylase 1 (TPH-1) and TPH-2, the genes and proteins related to the 5-HT signaling pathway as well as gut microbiota composition were determined. SWCA treatment alleviated bone loss and decreased serum levels of serotonin, which was negatively related to bone mineral density (BMD) in rats. It suppressed the protein expression of TPH-1 in the colon, and reversed the gene and protein expressions of FOXO1 and ATF4 in the femur in OVX rats, while it did not affect the TPH-2 protein expression in the cortex. SWCA treatment escalated the relative abundance of Antinobacteria and modulated several genera relating to BMD. These findings verified that the bone protective effects of lignans were mediated by serotonin, and provided evidence that lignans might be a good source of TPH-1 inhibitors.

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Short‐term exposure to synthetic flaxseed lignan LGM2605 alters gut microbiota in mice

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 7 publications

References 50 publications

Flaxseed Lignan Alleviates the Paracetamol-Induced Hepatotoxicity Associated with Regulation of Gut Microbiota and Serum Metabolome

Flaxseed Lignan Alleviates the Paracetamol-Induced Hepatotoxicity Associated with Regulation of Gut Microbiota and Serum Metabolome

Dietary Flaxseed and Flaxseed Oil Differentially Modulate Aspects of the Microbiota Gut–Brain Axis Following an Acute Lipopolysaccharide Challenge in Male C57Bl/6 Mice

The Lignan-Rich Fraction from Sambucus williamsii Hance Exerts Bone Protective Effects via Altering Circulating Serotonin and Gut Microbiota in Rats

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