Short-Term Insulin-Induced Glycogen Formation in Primary Hepatocytes as a Screening Bioassay for Insulin Action

Vu, Lan Phuong; Pralong, William; Cerini, Fabrice; Gjinovci, Asllan; Stöcklin, Reto; Rose, Keith; Offord, Robin E.; Kippen, Alistair D.

doi:10.1006/abio.1998.2731

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…Present analytical methods for the detection of insulin include bioassays, immunoassays and chromatography. The development of bioassays – and immunoassays – for the detection of insulin has been well reported in the literature. Of these, radioimmunoassays (RIA) – and enzyme-linked immunosorbent assays (ELISA) – are the most commonly used methods.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Study of Insulin at the Polarized Liquid−Liquid Interface

2008

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This paper reports on the electrochemical behavior of bovine insulin at the interface between two immiscible electrolyte solutions (ITIES). The voltammetric ion-transfer response obtained in the presence of insulin was dependent on the aqueous phase pH conditions and on the nature of the organic phase electrolyte employed in experiments. Optimal detection was obtained at acidic pH below the isoelectric point of insulin where it was positively charged. A shift in transfer potentials to lower potential values was observed with decreasing hydrophobicity of the anion of the organic phase electrolyte. No ion-transfer response was observed at pH values of the aqueous phase above the isoelectric point, where insulin was negatively charged. These results suggest that the voltammetric response is due to ion-pairing interactions at the ITIES between positively charged insulin and the hydrophobic anion of the organic phase electrolyte, together with adsorption of the ion-pair at the interface. The voltammetric response was obtained for insulin at concentrations down to 1 muM. These results show that electrochemistry is useful in studying the behavior of this important protein molecule at the polarized water-1,2-DCE interface and provides an alternative detection mode for bioanalytical applications.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Study of Insulin at the Polarized Liquid−Liquid Interface

2008

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“…1,2 Therefore, insulin measurement is an important subject for clinical diagnostics and physiological studies of this disease. The present analytical procedures for the determination of insulin include bioassay, 5 immunoassay, 6 chromatography 7 and capillary electrophoresis. 8 These methods can detect insulin down to sub-nM or even pM concentrations, but on the other hand, they are time-consuming, cumbersome and expensive.…”

Section: Introductionmentioning

confidence: 99%