Short‐term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord

Ding, Lei; Takebayashi, Hirohide; Watanabe, Keisuke; Ohtsuki, Toshiaki; Tanaka, Kenji F.; Nabeshima, Yo Ichi; Chisaka, Osamu; Ikenaka, Kazuhiro; Ono, Katsuhiko

doi:10.1002/dvdy.20545

Cited by 41 publications

(34 citation statements)

References 35 publications

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“…In situ hybridization was performed as described previously (Ding et al, 2005), using the following digoxygenin (DIG)-labeled RNA probes: mouse netrin-1, Unc5c (formerly Unc5h3), DCC, and chick netrin-1 (Sugimoto et al, 2001;Watanabe et al, 2006). DIG-labeled RNA probes were synthesized by using a DIG RNA-labeling kit (Roche Diagnostics).…”

Section: Methodsmentioning

confidence: 99%

Netrin-1 Acts as a Repulsive Guidance Cue for Sensory Axonal Projections toward the Spinal Cord

Masuda¹,

Watanabe²,

Sakuma³

et al. 2008

J. Neurosci.

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During early development, the ventral spinal cord expresses chemorepulsive signals that act on dorsal root ganglion (DRG) axons to help orient them toward the dorsolateral part of the spinal cord. However, the molecular nature of this chemorepulsion is mostly unknown. We report here that netrin-1 acts as an early ventral spinal cord-derived chemorepellent for DRG axons. In the developing mouse spinal cord, netrin-1 is expressed in the floor plate of the spinal cord, and the netrin receptor Unc5c is expressed in DRG neurons. We show that human embryonic kidney cell aggregates secreting netrin-1 repel DRG axons and that netrin-1-deficient ventral spinal cord explants lose their repulsive influence on DRG axons. In embryonic day 10 netrin-1 mutant mice, we find that DRG axons exhibit transient misorientation. Furthermore, by means of gain-of-function analyses, we show that ectopic netrin-1 in the dorsal and intermediate spinal cord prevents DRG axons from being directed toward the dorsal spinal cord. Together, these findings suggest that netrin-1 contributes to the formation of the initial trajectories of developing DRG axons as a repulsive guidance cue.

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Section: Methodsmentioning

confidence: 99%

Netrin-1 Acts as a Repulsive Guidance Cue for Sensory Axonal Projections toward the Spinal Cord

Masuda¹,

Watanabe²,

Sakuma³

et al. 2008

J. Neurosci.

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“…The expression of the murine bHLH gene Olig3 is first detected around embryonic day 9.25 (E9.25) in the dorsal hindbrain and spinal cord (Takebayashi et al, 2002b;Ding et al, 2005;Muller et al, 2005). We used immunohistochemistry to characterize Olig3 + cells on transverse sections of the hindbrain.…”

Section: + Cells In Rhombomeres 4-7mentioning

confidence: 99%

The bHLH transcription factor Olig3 marks the dorsal neuroepithelium of the hindbrain and is essential for the development of brainstem nuclei

et al. 2009

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The Olig3 gene encodes a bHLH factor that is expressed in the ventricular zone of the dorsal alar plate of the hindbrain. We found that the Olig3 + progenitor domain encompassed subdomains that co-expressed Math1, Ngn1, Mash1 and Ptf1a. Olig3 + cells give rise to neuronal types in the dorsal alar plate that we denote as class A neurons. We used genetic lineage tracing to demonstrate that class A neurons contribute to the nucleus of the solitary tract and to precerebellar nuclei. The fate of class A neurons was not correctly determined in Olig3 mutant mice. As a consequence, the nucleus of the solitary tract did not form, and precerebellar nuclei, such as the inferior olivary nucleus, were absent or small. At the expense of class A neurons, ectopic Lbx1 + neurons appeared in the alar plate in Olig3 mutant mice. By contrast, electroporation of an Olig3 expression vector in the chick hindbrain suppressed the emergence of Lbx1 + neurons. Climbing fiber neurons of the inferior olivary nucleus express Foxd3 and require Olig3 as well as Ptf1a for the determination of their fate. We observed that electroporation of Olig3 and Ptf1a expression vectors, but not either alone, induced Foxd3. We therefore propose that Olig3 can cooperate with Ptf1a to determine the fate of climbing fiber neurons of the inferior olivary nucleus.

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“…The presence of a 725 bp fragment, a 525 bp fragment, or both, indicated animals with tra/tra, +/+, and +/tra genotypes, respectively. Mice carrying the CP2L1 allele in which its exon 2 was flanked by two loxP sites were crossed with Pc36 Creexpressing mice (provided by H. Takebayashi) (Ding et al, 2005) to excise exon 2, leading to a frame-shift mutation that generates a null allele of Cp2l1 (see Fig. S1 in the supplementary material).…”

Section: Mouse Strainsmentioning

confidence: 99%

Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse

Yamaguchi

Yonemura²,

Takada

2006

Development

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Duct epithelial structure is an essential feature of many internal organs, including exocrine glands and the kidney. The ducts not only mediate fluid transfer but also help to maintain homeostasis. For instance, fluids and solutes are resorbed from or secreted into the primary fluid flowing through the lumen of the ducts in the exocrine glands and kidneys. The molecular mechanism underlying the functional maturation of these ducts remains largely unknown. Here, we show that a grainyhead-related transcription factor, CP2-like 1 (CP2L1), is required for the maturation of the ducts of the salivary gland and kidney. In the mouse, Cp2l1 is specifically expressed in the developing ducts of a number of exocrine glands, including the salivary gland, as well as in those of the kidney. In Cp2l1-deficient mice, the expression of genes directly involved in functional maturation of the ducts was specifically reduced in both the salivary gland and kidney, indicating that Cp2l1 is required for the differentiation of duct cells. Furthermore, the composition of saliva and urine was abnormal in these mice. These results indicate that Cp2l1 expression is required for normal duct development in both the salivary gland and kidney.

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Short‐term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord

Cited by 41 publications

References 35 publications

Netrin-1 Acts as a Repulsive Guidance Cue for Sensory Axonal Projections toward the Spinal Cord

Netrin-1 Acts as a Repulsive Guidance Cue for Sensory Axonal Projections toward the Spinal Cord

The bHLH transcription factor Olig3 marks the dorsal neuroepithelium of the hindbrain and is essential for the development of brainstem nuclei

Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse

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