Short-Term Preservation of Mouse Oocytes at 5.DEG.C..

Tsuchiya, Hideaki; Ogonuki, Narumi; Kuwana, Takashi; Sankai, Tadashi; Kanayama, Kiichi

doi:10.1538/expanim.50.441

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…On the other hand, when oocytes were stored at 37 C in an incubator, 64 to 83% of oocytes were externally normal, but none developed to the morula/blastocyst stage after parthenogenetic activation. Tsuchiya et al [21] reported that, when oocytes were stored at 5C for one day, 79% of stored oocytes were normal and 30% of stored oocytes developed to the morula/ blastocyst stages after in vitro fertilization. However, they did not specifically categorize the oocytes by gross morphology as we did; therefore, their designation of "normal" may differ certainly from ours.…”

Section: Discussionmentioning

confidence: 99%

“…For these reasons, only a few investigations of room temperature storage of oocytes have been conducted [21] and none have succeeded in producing viable offspring from such aged oocytes in vitro. In the present study, we demonstrate that alt hou gh m ost in v i tro a g e d o o c y t e s h av e irreversible damage to the metaphase-II spindle, at least some retained full developmental potential after storage for one day at room temperature.…”

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confidence: 99%

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Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

Wakayama

Thuan

Kishigami

et al. 2004

Journal of Reproduction and Development

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Abstract.To determine the feasibility of preserving oocytes without freezing, we stored mouse oocytes in several media at different temperatures for one day. Confocal microscopy of the metaphase-II spindle in these stored oocytes revealed gross abnormalities in both the spindle and the arrangement of chromosomes. The abnormal spindles could not be rescued by transplanting the aged spindle-chromosome complex into a fresh enucleated oocyte. A diploid parthenogenetic development showed that some of the oocytes stored at room temperature could still develop into blastocysts (10-57%). However, oocytes stored in a refrigerator (5%) or incubator (0%) lost the potential almost entirely. Fertilization of room-temperature-preserved oocytes with fresh spermatozoa by ICSI or IVF resulted in, respectively, 4 and 10%, full-term births. These results suggest that when oocytes are stored at room temperature for one day, most have irreversible damage not only to their cytoplasm but also to the spindle. However, since at least a few percent of stored oocytes retained the potential for full-term development, it may be possible to overcome these problems and develop a simple method for preserving mammalian oocytes without freezing.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

Wakayama

Thuan

Kishigami

et al. 2004

Journal of Reproduction and Development

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“…In several species, including rabbits (17), cows (18,19), sheep (20) and mice (21)(22)(23)(24)(25)(26)(27)(28)(29), successful refrigeration of preimplantation embryos at 0-7 C has been reported. Although short-term storage of unfertilized oocytes is not a common practice, there have been many studies on the refrigeration of fertilized oocytes in several mouse strains (5,22,26).…”

Section: Discussionmentioning

confidence: 99%

Birth after 12 hours of oocyte refrigeration

Coban¹,

Hacifazlioglu²,

Ciray³

et al. 2010

Fertility and Sterility

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“…1971); the osmolality of the medium is 290 ± 10 mOsm. Standard TYH medium for IVF is usually supplemented with 5.5 m m glucose during pre‐incubation and insemination (Tsuchiya et al. 2001).…”

Section: Medium Preparationmentioning

confidence: 99%

Effects of Glucose Concentration on in vitro Fertilization in BALB/c Mice

Chou

Lin

et al. 2003

Reprod Domestic Animals

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BALB/c mice are widely used in genetic, tumour and immunological studies. However, the mice demonstrate a lower reproduction rate, low fertility and small litters, because of their highly genetic homozygoisty. Based on in vitro fertilization (IVF), a routine technique for biomedical studies, it is worth to evaluate the effects to BALB/c mice on IVF efficiency. In order to test the genetic factor affecting the IVF efficiency of BALB/c, four reciprocal IVF tests of BALB/cByJ and FVB/NCrl mice were performed. The results showed that the average fertility of IVF sponsored by FVB/NCrl spermatozoa was 69.6%, but only 12.1% was obtained from BALB/cByJ strain. Effect of glucose contained in the culture medium to the IVF efficiency of BALB/cByJ was also evaluated. The results showed that the fertility of BALB/cByJ spermatozoa incubated with 0, 2.7, 5.5, 11.1 and 22.2 mm of glucose in the TYH medium were 6.8, 9.9, 13.9, 32.7 and 22.2%, respectively. It is showed that IVF efficiency of BALB/cByJ spermatozoa could be improved depending on the concentration of glucose in the IVF medium. According to the results, it is beleived that lower IVF of BALB/cByJ mice might be due to the genetic defect in spermatozoa and increasing glucose in the IVF medium which significantly affect the IVF efficiency of BALB/cByl via activating the spermatozoa.

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Short-Term Preservation of Mouse Oocytes at 5.DEG.C..

Cited by 9 publications

References 15 publications

Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

Birth after 12 hours of oocyte refrigeration

Effects of Glucose Concentration on in vitro Fertilization in BALB/c Mice

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