Short term protein supplementation during a long interval prostaglandin-based protocol for timed AI in sheep

Errandonea, N.; Fierro, S.; Viñoles, C.; Gil, J.; Banchero, Georgget; Olivera-Muzante, J.

doi:10.1016/j.theriogenology.2018.03.021

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…Anel et al (2005) also reported that farm, year and season, AI technique and technician are main factors affecting fertility after AI in dairy sheep. Recently, Errandonea et al (2018) made an interesting reference about the importance of a short-term protein supplementation in the animal diet before cervical timed artificial insemination (TAI) with fresh semen, reporting an improvement in ovulation rate, prolificacy and fecundity. Satisfactory results after AI in dairy sheep are also influenced by meteorological variables, so that parameters such as temperature or rainy season can affect pregnancy success.…”

Section: Introductionmentioning

confidence: 99%

Technical recommendations for artificial insemination in sheep

et al. 2019

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Artificial insemination (AI) was the first important biotechnology applied to improve the genetics of farm animals. It allows the rapid and massive diffusion of desirable characteristics of males with high productive potential. We describe the different types of estrus induction and synchronization techniques and the use of the AI with fresh, chilled or frozen semen. Through the adequacy of the protocols of estrus synchronization and AI to the different production systems, the efficient use of reproductive techniques is possible, reaching acceptable pregnancy rates. Summary of reproductive results obtained using cervical and laparoscopic AI are presented.

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Section: Introductionmentioning

confidence: 99%

Technical recommendations for artificial insemination in sheep

et al. 2019

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“…Male fertility is influenced by a multitude of intrinsic factors such as animal age and semen factors characteristics [ 21 , 32 ]; however, the factors related to ewes are as much or more important to fertility. The origin of the animals, the weather season in which they are inseminated [ 33 ], the protein nutritional inputs received by the ewes during the insemination process [ 34 ], and even weather conditions on the day of insemination [ 35 ] are other external factors that influence fertility. All of those factors, together with the small number of ewes inseminated per ram, explain the extreme variations in fertility per ram and insemination day.…”

Section: Resultsmentioning

confidence: 99%

Efficiency of Artificial Insemination at Natural Estrus in Organic Churra Ewes

Palacios

Abecia

Plaza

et al. 2022

Veterinary Sciences

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Hormonal treatments used in the artificial insemination (AI) of sheep can cause several physiological problems that can affect negatively fertility and animal health; however, AI protocols based on the detection of natural estrus offer a more sustainable option and can achieve high fertility. In this study, an AI protocol at natural estrus in organic Churra sheep was performed. In the first phase (AI protocol development), 125 ewes were exocervically inseminated, and their fertility was assessed based on the following factors: number of AI, physiological state, body condition, estrus detection–AI interval, and vaginal fluids in cervix. That protocol was repeated for six consecutive years. In all individuals, fertilities based on the timing of insemination after estrus detection were very high. Lactating ewes produced better results than did dry ewes, which was probably because of the better feeding of the former. In addition, double insemination increased the fertility of ewes whose estrus was detected within 16 h of onset. Body condition and amount of vaginal fluid were correlated with fertility. Exocervical inseminations at natural estrus can produce acceptable fertility and prolificity in Churra ewes.

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“…Moreover, in vitro fertility does not depend solely on the fertilizing capacity of the sperm, but also on the capacity of the oocyte to be fertilized. (31,32) On the other hand, the in vivo test depends largely on the fertility of the female, which is affected by a wide range of factors, such as farm, year, season, artificial insemination technique, and technician, (33) heat stress, (34)(35)(36) diet, (37) health status, as well as parity, lambing interval, body condition score, genetic traits, full functionality of reproductive organs, herd management, female prolificacy, nutritional management before and after artificial insemination, the type of estrus (natural or hormonally manipulated), and the site of deposition of the semen and climate factors. (38,39) On the other hand, the sperm parameters evaluated in this study were selected due to their predictive capacity in fertility.…”

Section: Discussionmentioning

confidence: 99%

Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen

Castellanos Zacarías,

Ramón Ugalde,

Loeza Concha

et al. 2023

Vet Mex OA

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The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation of two tropical hair breeds. The ejaculates were collected from 8 rams (4 Blackbelly and 4 Pelibuey), which were mixed (pooled) by breed, diluted and cryopreserved in three different extenders: Animal protein [Tris egg yolk (Triladyl®)], and animal protein-free extenders AndroMed® (lecithin soy bean) and OPTIXcell® (liposome). Sperm analyses of total (TM) and progressive (PM) motility, viability, mitochondrial activity, acrosome integrity, and plasma membrane integrity (PMI) were carried out at 0 and 6 h after semen thawing. OPTIXcell® and Triladyl® extenders showed similar results between them and differed with AndroMed® in TM, PM, viability, and PMI (P<0.0017). In the Blackbelly breed, the TM was higher (P<0.0159) in OPTIXcell® than in AndroMed®. In the Pelibuey breed, the OPTIXcell® and Triladyl® showed similar results between them and differed with AndroMed® in TM, PM, viability, mitochondrial activity, and PMI (P<0.0140). However, Triladyl® showed a higher percentage of sperm with intact acrosome than AndroMed® (P<0.0392). In both breeds, spermatic parameters decreased progressively over the incubation time similarly in all three extenders. In conclusion, OPTIXcell® and Triladyl® proved to be the best extenders to cryopreserve Blackbelly and Pelibuey ram semen. However, OPTIXcell® is an animal protein-free extender that decreases the risk of bacterial contamination, whereas Triladyl® is composed of animal protein (egg yolk), which may impact the fertilizing capacity of sperm.

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Short term protein supplementation during a long interval prostaglandin-based protocol for timed AI in sheep

Cited by 10 publications

References 31 publications

Technical recommendations for artificial insemination in sheep

Technical recommendations for artificial insemination in sheep

Efficiency of Artificial Insemination at Natural Estrus in Organic Churra Ewes

Effect of two animal protein-free extenders on cryopreservation of Pelibuey and Blackbelly ram semen

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